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01-07-2008 | Original Contribution

Intestinal Mucosa-Associated Microflora in Ulcerative Colitis Patients Before and After Restorative Proctocolectomy with an Ileoanal Pouch

Authors: Maristela G. Almeida, Ph.D., Desidério R. Kiss, Ph.D., Bruno Zilberstein, Ph.D., Alina G. Quintanilha, Ph.D., Magaly G. Teixeira, Ph.D., Angelita Habr-Gama, Ph.D.

Published in: Diseases of the Colon & Rectum | Issue 7/2008

Abstract

Purpose

This study was designed to identify the mucosa-associated microflora in patients with severe ulcerative colitis before and after restorative proctocolectomy with ileoanal pouch construction in comparison with historic controls.

Methods

Ten patients with a diagnosis of ulcerative colitis were evaluated. Mucus was collected during colonoscopy from all segments of the colon and terminal ileum before surgery, and from the ileal pouch two and eight months after ileostomy closure. The prevalence and mean concentration of the mucosa-associated microflora were compared over time and with historic controls.

Results

Veillonella sp was the most prevalent bacterium in patients and controls. Klebsiella sp was significantly more prevalent in the ileum of controls, was not found in patients with ulcerative colitis, and after proctocolectomy returned to values found in controls. Some bacteria such as Enterobacter sp, Staphylococcus sp (coag-), Bacteroides sp (npg), Lactobacillus sp, and Veillonella sp had higher mean concentrations in the ileal pouch of patients after surgery than in controls.

Conclusion

No bacterium was identified that could be exclusively responsible for the maintenance of the inflammatory process. The mucosa-associated microflora of patients with ulcerative colitis underwent significant changes after proctocolectomy with ileal pouch construction and returned to almost normal values for some bacteria.

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Title: Intestinal Mucosa-Associated Microflora in Ulcerative Colitis Patients Before and After Restorative Proctocolectomy with an Ileoanal Pouch
Authors: Maristela G. Almeida, Ph.D.
Desidério R. Kiss, Ph.D.
Bruno Zilberstein, Ph.D.
Alina G. Quintanilha, Ph.D.
Magaly G. Teixeira, Ph.D.
Angelita Habr-Gama, Ph.D.
Publication date: 01-07-2008
Publisher: Springer-Verlag
Published in: Diseases of the Colon & Rectum / Issue 7/2008
Print ISSN: 0012-3706
Electronic ISSN: 1530-0358
DOI: https://doi.org/10.1007/s10350-008-9347-8

At a glance: The STEP trials

Springer Medicine

Intestinal Mucosa-Associated Microflora in Ulcerative Colitis Patients Before and After Restorative Proctocolectomy with an Ileoanal Pouch

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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