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01-09-2007 | Original Article

Inter-laboratory validation of PCR-based HPV detection in pathology specimens

Authors: Iver Petersen, Christiane Schewe, Karsten Schlüns, Manfred Dietel, Norbert Speich, Christoph Schmitt, Magdolna Bollmann, Karl Sotlar, Burkhard Bültmann, Maria T. Dours-Zimmermann, Barbara Padberg, Dieter R. Zimmermann

Published in: Virchows Archiv | Issue 3/2007

Abstract

The detection and typing of human papilloma virus (HPV) in pathology specimens is gaining increasingly in importance. In the context of the initiative for quality assurance in pathology (QuIP) of the German Society of Pathology and the Professional Association of German Pathologists, four panel laboratories with experience and expertise in polymerase chain reaction (PCR)-based HPV detection were selected to establish an inter-laboratory trial. In a first step, these laboratories performed an internal testing of their own methodologies, which comprised DNA sequencing, multiplex nested PCR and hybridization techniques. Material from 39 samples including paraffin sections and DNA preparations of tissues and plasmids were evaluated by each panel institute according to their own protocols. Despite the different methodologies, a high degree of inter-laboratory reliability was achieved. In this report, we summarise the results. Pretested specimens are available for the external trail and can be ordered from the steering institute via provitro GmbH Berlin (http://www.provitro.de). Supplementary data are online available at http://pathologie-ccm.charite.de (rubric "Forschung"), which includes a web-based photo gallery of HPV-associated lesions and their potential association with specific virus types. The initiative is intended to foster the quality assurance of molecular HPV analysis in pathology and its correlation with morphological changes.

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Title: Inter-laboratory validation of PCR-based HPV detection in pathology specimens
Authors: Iver Petersen
Christiane Schewe
Karsten Schlüns
Manfred Dietel
Norbert Speich
Christoph Schmitt
Magdolna Bollmann
Karl Sotlar
Burkhard Bültmann
Maria T. Dours-Zimmermann
Barbara Padberg
Dieter R. Zimmermann
Publication date: 01-09-2007
Publisher: Springer-Verlag
Published in: Virchows Archiv / Issue 3/2007
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI: https://doi.org/10.1007/s00428-007-0437-0

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Inter-laboratory validation of PCR-based HPV detection in pathology specimens

Abstract

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Springer Medicine

Abstract

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