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Journal of Diabetes & Metabolic Disorders

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13-01-2023 | Insulins | Research article

Melatonin improves liver and pancreatic tissue injuries in diabetic rats: role on antioxidant enzymes

Authors: Onur Ertik, Bertan Boran Bayrak, Goksel Sener, Refiye Yanardag

Published in: Journal of Diabetes & Metabolic Disorders | Issue 1/2023

Abstract

Purpose

Melatonin (Mel) is an indolamine mainly synthesized by the pineal gland and many other organs. It plays an important role in scavenging free radicals and stimulating antioxidant enzymes. The goal of this study was to investigate the effect of Mel and/or insulin treatment on oxidative liver and pancreas injuries in diabetic rats.

Methods

Male Wistar albino rats were assigned into 5 groups. Group I: control animals. Group II: diabetes was induced via a single dose of STZ (60 mg/kg) administered intraperitoneally. Group III: diabetic rats treated with Mel (10 mg/kg/day). Group IV: diabetic rats given insulin (6U/kg) subcutaneously. Group V: diabetic rats that received insulin and Mel at the same dose and time. After 12 weeks of the experiment, the animals were decapitated, liver and pancreas tissues were collected.

Results

The results indicated that reduced glutathione levels in liver and pancreatic tissue decreased, while protein carbonyl, advanced oxidized protein products and lipid peroxidation levels were elevated in diabetic group. Antioxidant enzyme activities decreased in liver tissues but increased in pancreatic tissues of the diabetic group. Administration of Mel, insulin or Mel + insulin reversed these biochemical changes in the diabetic animals.

Conclusion

This work shows that in long-term oxidative stress conditions caused by STZ-induced diabetes, either Mel or Mel + insulin administration may improve the deteriorated oxidant/antioxidant system in both the liver and pancreas tissues. These results suggested that Mel alone or Mel + insulin treatments might have a significant role in protecting against liver and pancreatic damage in STZ diabetic rats via different antioxidant effects.

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Title: Melatonin improves liver and pancreatic tissue injuries in diabetic rats: role on antioxidant enzymes
Authors: Onur Ertik
Bertan Boran Bayrak
Goksel Sener
Refiye Yanardag
Publication date: 13-01-2023
Publisher: Springer International Publishing
Keywords: Insulins
Insulins
Published in: Journal of Diabetes & Metabolic Disorders / Issue 1/2023
Electronic ISSN: 2251-6581
DOI: https://doi.org/10.1007/s40200-022-01179-w

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