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01-01-2012 | Original Paper

Inhibitory activities of Puerariae Flos against testosterone 5α-reductase and its hair growth promotion activities

Authors: Kazuya Murata, Kazuma Noguchi, Masato Kondo, Mariko Onishi, Naoko Watanabe, Katsumasa Okamura, Hideaki Matsuda

Published in: Journal of Natural Medicines | Issue 1/2012

Abstract

Crude drugs expected to have an estrogenic effect were screened for their inhibitory activity on testosterone 5α-reductase. Testosterone 5α-reductase is an enzyme catalyzing the conversion of testosterone to dihydrotestosterone, which possesses high affinity for the androgen receptor. Among the crude drugs tested, we focused on Puerariae Flos (the flowers of Pueraria thomsonii) due to its potent inhibitory activity and suitability for commercial use. The 50% ethanolic extract of Puerariae Flos (PF-ext) showed inhibitory activity of 60.2% at 500 μg/ml against testosterone 5α-reductase. Interestingly, it was more potent than that of Puerariae Radix (roots of Pueraria lobata). PF-ext also showed in vivo anti-androgenic activity using a hair growth assay in testosterone-sensitive male C57Black/6NCrSlc strain mice. We demonstrated saponins, including soyasaponin I and kaikasaponin III, to be active components in PF-ext. In addition, hair growth promotion activity in C3H/He mice at 2 mg/mouse/day of the topical administration of PF-ext was demonstrated. Thus, Puerariae Flos is a promising crude drug for treating androgenic alopecia.

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Title: Inhibitory activities of Puerariae Flos against testosterone 5α-reductase and its hair growth promotion activities
Authors: Kazuya Murata
Kazuma Noguchi
Masato Kondo
Mariko Onishi
Naoko Watanabe
Katsumasa Okamura
Hideaki Matsuda
Publication date: 01-01-2012
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 1/2012
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-011-0570-6

At a glance: The STEP trials

Springer Medicine

Inhibitory activities of Puerariae Flos against testosterone 5α-reductase and its hair growth promotion activities

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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