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BMC Complementary Medicine and Therapies

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Open Access 01-12-2012 | Research article

Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

Authors: Huey-Chun Huang, Wan-Yu Hsieh, Yu-Lin Niu, Tsong-Min Chang

Published in: BMC Complementary Medicine and Therapies | Issue 1/2012

Abstract

Background

Magnolia grandiflora L. flower is wildly used in Asian as a traditional herbal medication. The purpose of the study was to investigate the antimelanogenic and antioxidant properties of Magnolia grandiflora L. flower extract. In the study, the inhibitory effects of M. grandiflora L. flower extract on mushroom tyrosinase, B16F10 intracellular tyrosinase activity and melanin content were determined spectrophotometrically. Meanwhile, the antioxidative capacity of the flower extract was also investigated.

Results

Our results revealed that M. grandiflora L. flower extract inhibit mushroom tyrosinase activity (IC₅₀ =11.1%; v/v), the flower extract also effectively suppressed intracellular tyrosinase activity (IC₅₀ = 13.6%; v/v) and decreased the amount of melanin (IC₅₀ = 25.6%; v/v) in a dose-dependent manner in B16F10 cells. Protein expression level of tyrosinase and tyrosinase-related protein 1 (TRP-1) were also decreased by the flower extract. Additionally, antioxidant capacities such as ABTS⁺ free radical scavenging activity, reducing capacity and total phenolic content of the flower extract were increased in a dose-dependent pattern.

Conclusions

Our results concluded that M. grandiflora L. flower extract decreased the expression of tyrosinase and TRP-1, and then inhibited melanogenesis in B16F10 cells. The flower extract also show antioxidant capacities and depleted cellular reactive oxygen species (ROS). Hence, M. grandiflora L. flower extract could be applied as a type of dermatological whitening agent in skin care products.

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The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/72/prepub

Title: Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract
Authors: Huey-Chun Huang
Wan-Yu Hsieh
Yu-Lin Niu
Tsong-Min Chang
Publication date: 01-12-2012
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2012
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/1472-6882-12-72

At a glance: The STEP trials

Springer Medicine

Inhibition of melanogenesis and antioxidant properties of Magnolia grandiflora L. flower extract

Abstract

Background

Results

Conclusions

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Results

Conclusions

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