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European Archives of Oto-Rhino-Laryngology
Published in: European Archives of Oto-Rhino-Laryngology 1/2012

01-01-2012 | Otology

Inhibition of fibroblast growth factor receptor 1: influence on tympanic membrane wound healing in rats

Authors: Holger Kaftan, Lars Reuther, Bärbel Miehe, Werner Hosemann, Achim Beule

Published in: European Archives of Oto-Rhino-Laryngology | Issue 1/2012

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Abstract

An animal model of chronic tympanic membrane (TM) perforation is needed for experiments on supporting healing of TM perforations. The basic fibroblast growth factor is important in TM wound healing. The object of this study was to investigate the efficacy of fibroblast growth factor receptor 1 (FGFR1) inhibition to arrest wound healing of experimental TM perforation. Bilateral instrumental myringotomies were performed in 12 rats. A specific inhibitor of the FGFR1 tyrosine kinase (SU5402) was applied to the left TM (2 mg/ml) and to the right TM (10 mg/ml) of each animal daily for 12 consecutive days. Thereafter, TMs were observed weekly for a total of 30 days. TM healing was delayed in a dose-dependent manner. We observed differences in the histologic parameters between both groups. SU 5402 is a strong inhibitor of TM healing but seems not to be suitable to create a chronic TM perforation in rat.
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Metadata
Title
Inhibition of fibroblast growth factor receptor 1: influence on tympanic membrane wound healing in rats
Authors
Holger Kaftan
Lars Reuther
Bärbel Miehe
Werner Hosemann
Achim Beule
Publication date
01-01-2012
Publisher
Springer-Verlag
Published in
European Archives of Oto-Rhino-Laryngology / Issue 1/2012
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI
https://doi.org/10.1007/s00405-011-1627-6

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