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Journal of Experimental & Clinical Cancer Research

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Open Access 01-01-2009 | Research

Inhibition of B16 melanoma growth and metastasis in C57BL mice by vaccination with a syngeneic endothelial cell line

Authors: Kenta Yoshiura, Toshihide Nishishita, Takashi Nakaoka, Naohide Yamashita, Naomi Yamashita

Published in: Journal of Experimental & Clinical Cancer Research | Issue 1/2009

Abstract

Background

Key role of angiogenesis in tumor growth and metastasis based on accumulating evidence and recent progress of immunotherapy have led us to investigate vaccine therapy targeting tumor angiogenesis.

Methods

C57BL/6J mice were vaccinated with a syngeneic endothelial cell line Tpit/E by subcutaneous injection once a week. Prior to ninth vaccination, the mice were challenged with B16/F10 melanoma cells by subcutaneous inoculation on the back for the tumor growth model or by tail venous injection for the lung metastasis model. Development of subcutaneous tumor and lung metastasis was monitored by computed tomography scanning, which enabled accurate evaluation with the minimized sacrifice of mice.

Results

Vaccination with Tpit/E cells inhibited subcutaneous tumor growth and appearance of lung metastasis compared to control. Survival period was elongated in the Tpit/E vaccination in both of the two models. We also obtained hybridomas secreting specific antibodies to Tpit/E cells from a mouse vaccinated with the cells, indicating that specific immune response to the syngeneic endothelial cells was elicited.

Conclusion

These results suggest that vaccination with an autologous endothelial cell line may be effective against melanoma.

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Title: Inhibition of B16 melanoma growth and metastasis in C57BL mice by vaccination with a syngeneic endothelial cell line
Authors: Kenta Yoshiura
Toshihide Nishishita
Takashi Nakaoka
Naohide Yamashita
Naomi Yamashita
Publication date: 01-01-2009
Publisher: BioMed Central
Published in: Journal of Experimental & Clinical Cancer Research / Issue 1/2009
Electronic ISSN: 1756-9966
DOI: https://doi.org/10.1186/1756-9966-28-13

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Abstract

Background

Methods

Results

Conclusion

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