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01-03-2021 | Original Article

Influence of de-remineralization process on chemical, microstructural, and mechanical properties of human and bovine dentin

Authors: Tattiana Enrich-Essvein, Cristina Benavides-Reyes, Pedro Álvarez-Lloret, María Victoria Bolaños-Carmona, Alejandro B. Rodríguez-Navarro, Santiago González-López

Published in: Clinical Oral Investigations | Issue 3/2021

Abstract

Objectives

This study compared the chemical composition, microstructural, and mechanical properties of human and bovine dentin subjected to a demineralization/remineralization process.

Materials and methods

Human and bovine incisors were sectioned to obtain 120 coronal dentin beams (6 × 1 × 1 mm³) that were randomly allocated into 4 subgroups (n = 15) according to the time of treatment (sound, pH-cycling for 3, 7, and 14 days). Three-point bending mechanical test, attenuated total reflectance–Fourier transform infrared (ATR-FTIR), thermogravimetric (TG), and X-ray diffraction (XRD) techniques were employed to characterize the dentin samples.

Results

Regarding chemical composition at the molecular level, bovine sound dentin showed significantly lower values in organic and inorganic content (collagen cross-linking, CO₃/amide I, and CO₃/PO₄; p = 0.002, p = 0.026, and p = 0.002, respectively) compared to humans. Employing XRD analyses, a higher mineral crystallinity in human dentin than in bovines at 7 and 14 days (p = 0.003 and p = 0.009, respectively) was observed. At the end of the pH-cycling, CI (ATR-FTIR) and CO₃/PO₄ ratios (ATR-FTIR) increased, while CO₃/amide I (ATR-FTIR), PO₄/amide I (ATR-FTIR), and %mineral (TG) ratios decreased. The extension by compression values increased over exposure time with significant differences between dentin types (p < 0.001, in all cases), reaching higher values in bovine dentin. However, flexural strength (MPa) did not show differences between groups. We also observed the correlation between compositional variables (i.e., PO₄/amide I, CI, and %mineral) and the extension by compression.

Conclusions

Human and bovine dentin are different in terms of microstructure, chemical composition, mechanical strength, and in their response to the demineralization/remineralization process by pH-cycling.

Clinical relevance

These dissimilarities may constitute a potential limitation when replacing human teeth with bovines in in vitro studies.

Yassen GH, Platt JA, Hara AT (2011) Bovine teeth as substitute for human teeth in dental research: a review of literature. J Oral Sci 53:273–282. https://doi.org/10.2334/josnusd.53.273CrossRefPubMed

Soares FZM, Follak A, da Rosa LS, Montagner AF, Lenzi TL, Rocha RO (2016) Bovine tooth is a substitute for human tooth on bond strength studies: a systematic review and meta-analysis of in vitro studies. Dent Mater 32:1385–1393. https://doi.org/10.1016/j.dental.2016.09.019CrossRefPubMed

Nakamichi I, Iwaku M, Fusayama T (1983) Bovine teeth as possible substitutes in the adhesion test. J Dent Res 62:1076–1081. https://doi.org/10.1177/00220345830620101501CrossRefPubMed

Wegehaupt FJ, Widmer R, Attin T (2010) Is bovine dentine an appropriate substitute in abrasion studies? Clin Oral Investig 14:201–205. https://doi.org/10.1007/s00784-009-0283-3CrossRefPubMed

Tanaka JLO, Medici Filho E, Salgado JAP, Salgado MAC, Moraes LC, Moraes MEL, Castilho JCM (2008) Comparative analysis of human and bovine teeth: radiographic density. Braz Oral Res 22:346–351. https://doi.org/10.1590/S1806-83242008000400011CrossRefPubMed

Dutra-Correa M, Anauate-Netto C, Arana-Chavez VE (2007) Density and diameter of dentinal tubules in etched and non-etched bovine dentine examined by scanning electron microscopy. Arch Oral Biol 52:850–855. https://doi.org/10.1016/j.archoralbio.2007.03.003CrossRefPubMed

Lopes MB, Sinhoreti MAC, Gonini Júnior A, Consani S, Mccabe JF (2009) Comparative study of tubular diameter and quantity for human and bovine dentin at different depths. Braz Dent J 20:279–283. https://doi.org/10.1590/S0103-64402009000400003CrossRefPubMed

Fonseca RB, Haiter-Neto F, Fernandes-Neto AJ, Barbosa GAS, Soares CJ (2004) Radiodensity of enamel and dentin of human, bovine and swine teeth. Arch Oral Biol 49:919–922. https://doi.org/10.1016/j.archoralbio.2004.05.006CrossRefPubMed

Marquezan M, Corrêa FNP, Sanabe ME, Rodrigues Filho LE, Hebling J, Guedes-Pinto AC, Mendes FM (2009) Artificial methods of dentine caries induction: a hardness and morphological comparative study. Arch Oral Biol 54:1111–1117. https://doi.org/10.1016/j.archoralbio.2009.09.007CrossRefPubMed

10.

White DJ (1995) The application of in vitro models to research on demineralization and remineralization of the teeth. Adv Dent Res 9:175–193CrossRefPubMed

11.

Pacheco LF, Banzi ÉC d F, Rodrigues E et al (2013) Molecular and structural evaluation of dentin caries-like lesions produced by different artificial models. Braz Dent J 24:610–618. https://doi.org/10.1590/0103-6440201302357CrossRefPubMed

12.

Boskey AL, Mendelsohn R (2005) Infrared spectroscopic characterization of mineralized tissues. Vib Spectrosc 38:107–114. https://doi.org/10.1016/j.vibspec.2005.02.015CrossRefPubMedPubMedCentral

13.

Lopes C d CA, Limirio PHJO, Novais VR, Dechichi P (2018) Fourier transform infrared spectroscopy (FTIR) application chemical characterization of enamel, dentin and bone. Appl Spectrosc Rev 53:747–769. https://doi.org/10.1080/05704928.2018.1431923CrossRef

14.

Rodriguez-Navarro A, Romanek C, Alvarez-LLoret P, Gaines K (2006) Effect of in ovo exposure to PCBs and Hg on clapper rail bone mineral chemistry from a contaminated salt marsh in coastal Georgia. Environ Sci Technol 40:4936–4942. https://doi.org/10.1021/es060769xCrossRefPubMed

15.

Miller LM, Vairavamurthy V, Chance MR, Mendelsohn R, Paschalis EP, Betts F, Boskey AL (2001) In situ analysis of mineral content and crystallinity in bone using infrared micro-spectroscopy of the ν4 PO43− vibration. Biochim Biophys Acta, Gen Subj 1527:11–19. https://doi.org/10.1016/S0304-4165(01)00093-9CrossRef

16.

Paschalis EP, Verdelis K, Doty SB, Boskey AL, Mendelsohn R, Yamauchi M (2001) Spectroscopic characterization of collagen cross-links in bone. J Bone Miner Res 16:1821–1828. https://doi.org/10.1359/jbmr.2001.16.10.1821CrossRefPubMed

17.

Holager J (1970) Thermogravimetric examination of enamel and dentin. J Dent Res 49:546–548CrossRefPubMed

18.

Lim JJ, Liboff AR (1972) Thermogravimetric analysis of dentin. J Dent Res 51:509–514. https://doi.org/10.1177/00220345720510024401CrossRefPubMed

19.

Elfersi S, Grégoire G, Sharrock P (2002) Characterization of sound human dentin particles of sub-millimeter size. Dent Mater 18:529–534. https://doi.org/10.1586/ecp.10.28CrossRefPubMed

20.

Reyes-Gasga J, Martínez-Piñeiro EL, Rodríguez-Álvarez G, Tiznado-Orozco GE, García-García R, Brès EF (2013) XRD and FTIR crystallinity indices in sound human tooth enamel and synthetic hydroxyapatite. Mater Sci Eng C 33:4568–4574. https://doi.org/10.1016/j.msec.2013.07.014CrossRef

21.

Gadaleta SJ, Paschalis EP, Betts F, Mendelsohn R, Boskey AL (1996) Fourier transform infrared spectroscopy of the solution-mediated conversion of amorphous calcium phosphate to hydroxyapatite: new correlations between X-ray diffraction and infrared data. Calcif Tissue Int 58:9–16. https://doi.org/10.1007/BF02509540CrossRefPubMed

22.

Hara AT, Queiroz CS, Paes Leme AF, Serra MC, Cury JA (2003) Caries progression and inhibition in human and bovine root dentine in situ. Caries Res 37:339–344. https://doi.org/10.1159/000072165CrossRefPubMed

23.

De Dios TJ, Alcolea A, Hernández A, Ruiz AJO (2015) Comparison of chemical composition of enamel and dentine in human, bovine, porcine and ovine teeth. Arch Oral Biol 60:768–775. https://doi.org/10.1016/j.archoralbio.2015.01.014CrossRef

24.

Silva Soares LE, Do Espírito Santo AM (2015) Morphological and chemical comparative analysis of the human and bovine dentin-adhesive layer. Microsc Microanal 21:204–213. https://doi.org/10.1017/S143192761401366XCrossRef

25.

Falla-Sotelo FO, Rizzutto MA, Tabacniks MH, Added N, Barbosa MDL, Markarian RA, Quinelato A, Mori M, Youssef M (2005) Analysis and discussion of trace elements in teeth of different animal species. Braz J Phys 35:761–762. https://doi.org/10.1590/S0103-97332005000500010CrossRef

26.

Soares LES, Campos ADF, Martin AA (2013) Human and bovine dentin composition and its hybridization mechanism assessed by FT-Raman spectroscopy. J Spectrosc 2013:1–7. https://doi.org/10.1155/2013/210671CrossRef

27.

Dawes C (2003) What is the critical pH and why does a tooth dissolve in acid? J Can Dent Assoc 69:722–725PubMed

28.

Ito A, Maekawa K, Tsutsumi S, Ikazaki F, Tateishi T (1997) Solubility product of OH-carbonated hydroxyapatite. J Biomed Mater Res 36:522–528. https://doi.org/10.1002/(SICI)1097-4636(19970915)36:4<522::AID-JBM10>3.0.CO;2-CCrossRefPubMed

29.

Ortiz-Ruiz AJ, Teruel-Fernández J d D, Alcolea-Rubio LA et al (2018) Structural differences in enamel and dentin in human, bovine, porcine, and ovine teeth. Ann Anat 218:7–17. https://doi.org/10.1016/j.aanat.2017.12.012CrossRefPubMed

30.

Barralet JE, Best S, Bonfield W (1998) Carbonate substitution in precipitated hydroxyapaptite: an investigation into the effects of reaction temperature and bicarbonae ion concentration. J Biomed Mater Res 41:79–86CrossRefPubMed

31.

Rey C, Collins B, Goehl T, Dickson IR, Glimcher MJ (1989) The carbonate environment in bone mineral: a resolution-enhanced fourier transform infrared spectroscopy study. Calcif Tissue Int 45:157–164. https://doi.org/10.1007/BF02556059CrossRefPubMed

32.

Donnelly E, Boskey AL, Baker SP, van der Meulen MCH (2010) Effects of tissue age on bone tissue material composition and nanomechanical properties in the rat cortex. J Biomed Mater Res A 92:1048–1056. https://doi.org/10.1002/jbm.a.32442CrossRefPubMedPubMedCentral

33.

Kinney J, Balooch M, Haupt D et al (1995) Mineral distribuition and dimensional changes in human dentin during demineralization. J Dent Res 74:1179–1184CrossRefPubMed

34.

Almahdy A, Downey FC, Sauro S, Cook RJ, Sherriff M, Richards D, Watson TF, Banerjee A, Festy F (2012) Microbiochemical analysis of carious dentine using raman and fluorescence spectroscopy. Caries Res 46:432–440. https://doi.org/10.1159/000339487CrossRefPubMed

35.

Cazalbou S, Combes C, Eichert D et al (2004) Poorly crystalline apatites: evolution and maturation in vitro and in vivo. J Bone Miner Metab 22:310–317. https://doi.org/10.1007/s00774-004-0488-0CrossRefPubMed

36.

Dominguez-Gasca N, Benavides-Reyes C, Sánchez-Rodríguez E, Rodríguez-Navarro AB (2019) Changes in avian cortical and medullary bone mineral composition and organization during acid-induced demineralization. Eur J Mineral 31:209–216. https://doi.org/10.1127/ejm/2019/0031-2826CrossRef

37.

Ryou H, Amin N, Ross A, Eidelman N, Wang DH, Romberg E, Arola D (2011) Contributions of microstructure and chemical composition to the mechanical properties of dentin. J Mater Sci Mater Med 22:1127–1135. https://doi.org/10.1007/s10856-011-4293-8CrossRefPubMedPubMedCentral

38.

Schilke R, Lisson JA, Bauß O, Geurtsen W (2000) Comparison of the number and diameter of dentinal tubules in human and bovine dentine by scanning electron microscopic investigation. Arch Oral Biol 45:355–361. https://doi.org/10.1016/S0003-9969(00)00006-6CrossRefPubMed

39.

Camargo CHR, Siviero M, Camargo SEA, de Oliveira SHG, Carvalho CAT, Valera MC (2007) Topographical, diametral, and quantitative analysis of dentin tubules in the root canals of human and bovine teeth. J Endod 33:422–426. https://doi.org/10.1016/J.JOEN.2006.12.011CrossRefPubMed

40.

Ferreira M, De Carvalho F, Neiva C (2018) Viability of bovine teeth as a substrate in bond strength tests : a systematic review and meta-analysis. J Adhes Dent 20:471–480. https://doi.org/10.3290/j.jad.a41636CrossRef

Title: Influence of de-remineralization process on chemical, microstructural, and mechanical properties of human and bovine dentin
Authors: Tattiana Enrich-Essvein
Cristina Benavides-Reyes
Pedro Álvarez-Lloret
María Victoria Bolaños-Carmona
Alejandro B. Rodríguez-Navarro
Santiago González-López
Publication date: 01-03-2021
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 3/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03371-9

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Influence of de-remineralization process on chemical, microstructural, and mechanical properties of human and bovine dentin

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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