Published in:
01-01-2009
Influence of carbon dioxide pneumoperitoneum environment on adhesion and metastasis of a human ovarian cancer cell line
Published in: Surgical Endoscopy | Issue 1/2009
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Background
This study aimed to explore the adhesion and metastasis capability of the human ovarian cancer cell line SKOV3 after exposure to a simulated laparoscopic carbon dioxide (CO2) pneumoperitoneum environment and the related mechanism.
Methods
SKOV3 was subjected to a simulated laparoscopic CO2 pneumoperitoneum environment at various CO2 pressures (8–12 mmHg) and exposure times (1–3 h). Cell adhesive capacity was determined by a mechanical method. Real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) and immunocytochemical staining were used to examine the mRNA and protein expressions of heparanase (HPSE) and vascular endothelial growth factor C (VEGF-C) in SKOV3. Cells cultured in a standard environment were used as the control.
Results
The increase of SKOV3 cell adhesion capability was associated with CO2 pressure and exposure time. A significantly higher adhesion capability was observed in the group with exposure to 10 mmHg for 2 h over a 48 h period, as compared with the control groups (p < 0.05). The expressions of HPSE and VEGF-C in SKOV3, which are closely related to metastasis capability, also increased. Significantly higher expressions were observed in the group with exposure to 10 mmHg for 3 h over a 48 h period, as compared with the control groups (p < 0.01).
Conclusions
The adhesion and metastasis capacity of SKOV3 increased with overexpression of HPSE and VEGF-C and were positively related to CO2 pressure, exposure, and culture time.