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Clinical Oral Investigations
Published in: Clinical Oral Investigations 4/2017

01-05-2017 | Original Article

Increased visfatin expression is associated with nuclear factor-kappa B and phosphatidylinositol 3-kinase in periodontal inflammation

Authors: Erkan Özcan, N Işıl Saygun, Rahşan Ilıkçı, Yıldırım Karslıoğlu, Uğur Muşabak, Sait Yeşillik

Published in: Clinical Oral Investigations | Issue 4/2017

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Abstract

Objective

Visfatin is an adipocytokine that plays a role in regulating periodontal inflammation by as yet identified mechanisms. It has been suggested that visfatin mediates inflammation via activation of the nuclear factor-kappa B (NF-κB) and phosphatidylinositol 3-kinase (PI3k) signaling pathways which play a role in the inhibition of neutrophil apoptosis. The aim of this study was to investigate the expression of visfatin, NF-κB (NF-κB1 and NF-κB2), PI3k, tumor necrosis factor alpha (TNF-α), and interleukin-1 beta (IL-1β) in the tissue of healthy individuals and patients with periodontitis.

Materials and methods

Tissue biopsy samples were obtained from 21 patients with chronic periodontitis and from the gingiva of 19 healthy individuals undergoing crown lengthening. The mRNA expression levels of visfatin, NF-κB, PI3k, TNF-α, and IL-1β were evaluated by quantitative real-time PCR (qPCR). Also, visfatin protein expression was measured by immunohistochemistry.

Results

Both qPCR and immunohistochemistry results revealed that the visfatin expression was higher in the tissues with periodontitis than in healthy tissues (P < 0.01). Similarly, the mRNA expression levels of NF-κB2, PI3k, and IL-1β were higher in tissues with periodontitis than in healthy gingival tissues (P < 0.01). Visfatin was positively correlated with the levels of NF-κB1 (r = 0.549, P < 0.05), NF-κB2 (r = 0.636, P < 0.05), PI3k (r = 0.682, P < 0.01), TNF-α (r = 0.558, P < 0.05), and IL-1β (r = 0.686, P < 0.01) in the tissues with periodontitis.

Conclusions

Our results demonstrated that increased visfatin was associated with the expression of NF-κB and PI3k which may play a role in the pathogenesis of periodontitis. We suggest that increased visfatin may contribute to the inhibition of neutrophil apoptosis via the NF-κB and PI3k signaling pathways.

Clinical relevance

Understanding the role of visfatin in periodontitis will enable the development of new treatment methods for inflammation.
Footnotes
1
Tri-PureI, Roche, USA
 
2
Maestrogen, Taiwan
 
3
Roche, USA
 
4
Corbett Research, Germany
 
5
Roche Life Science, USA
 
6
Ventana, Benchmark XT, USA
 
7
Abcam, Cambridge, USA
 
8
Olympus BX-50, USA
 
Literature
1.
2.
Sarkar A, Hallberg L, Bhattacharyya A, et al. (2012) Infection with Anaplasma phagocytophilum activates the phosphatidylinositol 3-kinase/Akt and NF-κB survival pathways in neutrophil granulocytes. Infect Immun. doi:10.​1128/​IAI.​05219-11 PubMedPubMedCentral
3.
Malam Z, Parado J, Waheed F, et al. (2011) Pre-B cell colony-enhancing factor (PBEF/Nampt/visfatin) primes neutrophils for augmented respiratory burst activity through partial assembly of the NADPH oxidase. J Immunol 186:6474–6484CrossRefPubMed
4.
Shivanaikar SS, Faizuddin M, Bhat K (2013) Effect of smoking on neutrophils apoptosis in chronic periodontitis: an immunohistochemical study. Indian J Dent Res 24:147CrossRefPubMed
5.
Gamonal J, Sanz M, Connor AO, Acevedo A, Suarez I, Sanz A (2003) Delayed neutrophil apoptosis in chronic periodontitis patients. J Clin Periodontol 30:616–623CrossRefPubMed
6.
Zaric S, Shelburne C, Darveau R, Quinn DJ, Weldon S, Taggrat CC, Coulter WA (2010) Impaired immune tolerance to Porhyromonas gingivalis lipopolysaccharide promotes neutrophils migration and decreased apoptosis. Infect Immun 8:4151–4156CrossRef
7.
8.
Nepal S, Park PH (2015) Modulation of cell death and survival by adipokines in the liver. Curr Top 38:961–965
9.
10.
Oiata RC, Ferdinando D, Wilson S, et al. (2010) Visfatin induces oxidative stress in differentiated C2C12 myotubes in an Akt and MAPK independent, NF-κB dependent manner. Eur J Phys 459:619–630CrossRef
11.
Ghallab NA, Amr EM, Shaker OG (2015) Expression of leptin and visfatin in gingival tissues of chronic periodontitis with and without type 2 diabetes mellitus: a study utilizing enzyme linked immunosorbent assay and real-time polymerase chain reaction. J Periodontol 86:882–889CrossRefPubMed
12.
Pradeep A, Raghavendra N, Prasad MR, et al. (2011) Gingival crevicular fluid and serum visfatin concentration: their relationship in periodontal health and disease. J Periodontol 82:1314–1319CrossRefPubMed
13.
Özcan E, Saygun NI, Serdar MA, et al. (2014) Evaluation of the salivary levels of visfatin, chemerin, and progranulin in periodontal inflammation. Clin Oral Investig 19:921–928CrossRefPubMed
14.
Wu Y, Chen L, Wei B, et al. (2015) Effect of non-surgical periodontal treatment on visfatin concentrations in serum and gingival crevicular fluids of patients with chronic periodontitis and type 2 diabetes mellitus. J Periodontol 86:795–800CrossRefPubMed
15.
Deschner J, Eick S, Damanaki A, et al. (2014) The role of adipokines in periodontal infection and healing. Mol Oral Microbiol 29:258–269CrossRefPubMed
16.
Pradeep A, Raghavendra N, Sharma A, et al. (2012) Association of serum and crevicular visfatin levels in periodontal health and disease with type 2 diabetes mellitus. J Periodontol 83:629–634CrossRefPubMed
17.
Xiao K, Zou WH, Yang Z, et al. (2015) The role of visfatin on the regulation of inflammation and apoptosis in the spleen of LPS-treated rats. Cell Tissue Res 359:605–618CrossRefPubMed
18.
Akhisa M, Lang YW, Asha J, et al. (2014) FK 866, a visfatin inhibitor, protects against acute lung injury after intestinal ischemia–reperfusion in mice via NF-(kappa) B pathway. Ann Surg 295:1007–1017
19.
Jia SH, Li Y, Parodo J, et al. (2004) Pre-B cell colony-enhancing factor inhibits neutrophil apoptosis in experimental inflammation and clinical sepsis. J Clin Invest 113:1318–1327CrossRefPubMedPubMedCentral
20.
Roberts KJ, Cross A, Vasievra O, et al. (2013) Inhibition of pre-B cell colony-enhancing factor (PBEF/Nampt/visfatin) decreases the ability of human neutrophils to generate reactive oxidants but not impair bacterial killing. J Leukoc Biol 94:481–492CrossRefPubMed
21.
Suhaimi EA, Shehzad A (2013) Leptin, resistin and visfatin: the missing link between endocrine metabolic disorders and immunity. Eur J Med Res 18:1–13CrossRef
22.
Adya R, Chen J, Tan BK, et al. (2008) Nuclear factor –κB induction by visfatin in human vascular endothelial cells. Diabetes Care 31:758–760CrossRefPubMed
23.
Ambilli R, Santhi WS, Janam P, et al. (2005) Expression of activated transcription factor nuclear factor-kB in periodontally diseased tissues. J Periodontol 76:1148–1153CrossRef
24.
Buldak RJ, Polaniak R, Buldak L, et al. (2013) Exogenous administration of visfatin affects cytokines secretion and increase oxidative stress in human malignant melanoma ME45 cells. J Phisiol Pharm 64:377–385
25.
Arabacı T, Çiçek Y, Çanakçı V, et al. (2010) Immunohistochemical and stereologic analysis of NF-κB activation in chronic periodontitis. Eur J Dent 4:454–461PubMedPubMedCentral
26.
Dupont J, Reverchon M, Cloix L, et al. (2012) Involvement of adipokines, AMPK, PI3K and the PPAR signaling pathways in ovarian follicle development and cancer. Int J Dev Biol 56:959–967CrossRefPubMed
27.
Sarkar A, Möller S, Bhattarcharyya A, et al. (2015) Mechanisms of apoptosis in inhibition in Chlamydia pneumonia-infected neutrophils. Int J Med Microbiol 305:493–500CrossRefPubMed
28.
Cheng Q, Dong W, Qian L, et al. (2011) Visfatin inhibits apoptosis of pancreatic β-cell line, MIN6, via the mitogen activated protein kinase/phosphoinositide 3-kinase pathway. J Mol Endocrinol 47:13–21CrossRefPubMed
29.
Park SY, Park DJ, Kim YH, et al. (2011) Upregulation of heme oxygenase-1 via PI3K/Akt and Nrf-2 signaling pathways mediates the anti-inflammatory activity of Chisandrin in Porphyromonas gingivalis LPS-stimulated macrophage. Immunol Lett 139:93–101CrossRefPubMed
30.
Jeon YJ, Kim BH, Kim S, et al. (2013) Rhododendrin ameliorates skin inflammation through inhibition of NF-kB, MAPK, and PI3K/Akt signaling. Eur J Pharmacol 714:7–14CrossRefPubMed
31.
Kim SR, Bae YH, Bae SK, et al. (2008) Visfatin enhances ICAM-1 and VCAM-1 expression through ROS-dependent nuclear factor –κB activation in endothelial cells. Biochim Biophys Acta 1783:886–895CrossRefPubMed
32.
Lowren F, Pan Y, Shukla PC, Quan A, et al. (2009) Visfatin activates eNOS via Akt and MAP kinases and improves endothelial cell function and angiogenesis in vitro and in vivo: translational implications for atherosclerosis. Am J Physiol Endocrinol Metab 296:1440–1449CrossRef
33.
Silness J, Löe H (1964) Periodontal disease in pregnancy II. Correlation between oral hygiene and periodontal condition. Acta Odontol Scand 22:121–135CrossRefPubMed
34.
Löe H, Silness J (1963) Periodontal disease in pregnancy I. Prevalence and severity. Acta Odontol Scand 21:533–551CrossRefPubMed
35.
WHO expert consultation (2004) Appropriate Asian body mass index for Asian populations and its implications for policy and intervention strategies. Lancet 363:157–163CrossRef
36.
Blouch G, Toma DP, Robinson GE (2001) Behavioral rhythmicity, age, division of labor and period expression in the honey bee brain. J Biol Rhythm 16:444–456CrossRef
37.
Hamzaoui K, Houman H, Ben Dhifallah I, et al. (2008) Serum BAFF levels and skin mRNA expression in patients with Behçet’s disease. Clin Exp Rheumatol 26:64–71
38.
Kirkwood KL, Li F, Rogers J, et al. (2007) A p38 alpha selective mitogen activated protein kinase inhibitor prevents periodontal bone loss. J Pharmacol Exp Ther 320:55–67
39.
Travan S, Li F, Silva NJ, et al. (2013) Differential expression of mitogen activating protein kinases in periodontitis. J Clin Periodontol 40:757–764CrossRefPubMedPubMedCentral
40.
Raghavendra N, Pradeep A, Kathariya R, et al. (2012) Effect of non surgical periodontal therapy on gingival crevicular fluid and serum visfatin concentration in periodontal health and disease. Dis Markers 32:383–388CrossRefPubMedPubMedCentral
41.
42.
Nogueira AVB, Nokhbehsaim M, Eick S, et al. (2014) Regulation of visfatin by microbial and biomechanical signals in PDL cells. Clin Oral Investig 18:171–178CrossRefPubMed
43.
Özcan E, Saygun NI, Serdar MA, et al. (2015) Porphyromonas gingivalis and Epstein–Barr virus are associated with increased levels of visfatin in gingival crevicular fluid. J Periodontol 12:1–14
44.
45.
46.
Fan Y, Meng S, Wang Y, et al. (2011) Visfatin /PBEF/Nampt induces EMMPRIN and MMP-9 production in macrophages via the NAMPT-MAPK (P38-ERK ½)-NF-κB signaling pathway. Int J Mol Med 27:607–615CrossRefPubMed
47.
Adya R, Tan BK, Punn A, et al. (2008) Visfatin induces human endothelial VEGF and MMP-2/9 production via MAPK and PI3K/Akt signalling pathways: novel insights into visfatin-induced angiogenesis. Cardiovasc Res 78:356–365CrossRefPubMed
48.
Maggini J, Raiden G, Salamone A, et al. (2009) Regulation of neutrophils apoptosis by cytokines, pathogens and environmental stressors. Front Biosci 14:2372–2385CrossRef
Metadata
Title
Increased visfatin expression is associated with nuclear factor-kappa B and phosphatidylinositol 3-kinase in periodontal inflammation
Authors
Erkan Özcan
N Işıl Saygun
Rahşan Ilıkçı
Yıldırım Karslıoğlu
Uğur Muşabak
Sait Yeşillik
Publication date
01-05-2017
Publisher
Springer Berlin Heidelberg
Published in
Clinical Oral Investigations / Issue 4/2017
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI
https://doi.org/10.1007/s00784-016-1871-7

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