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Surgical Endoscopy

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01-06-2010

Increased mRNA expression of epidermal growth factor receptor, human epidermal receptor, and survivin in human gastric cancer after the surgical stress of laparotomy versus carbon dioxide pneumoperitoneum in a murine model

Authors: Anwar Tawfik Amin, Norio Shiraishi, Shigeo Ninomiya, Masaaki Tajima, Masafumi Inomata, Seigo Kitano

Published in: Surgical Endoscopy | Issue 6/2010

Abstract

Background

Surgical impact may be associated with enhanced tumor growth and chemoresistance. This study aimed to evaluate the effect of surgical impact on the mRNA expression of survivin, epidermal growth factor receptor (EGFR), and human epidermal receptor (HER2) in tumors after pneumoperitoneum versus laparotomy.

Methods

Nude mice were inoculated intraperitoneally with human gastric cancer cells (MKN45). Then laparotomy, carbon dioxide (CO₂) pneumoperitoneum, and anesthesia alone were performed randomly, after which EGFR, HER2, and survivin mRNA expression using reverse transcription-polymerase chain reaction (RT-PCR) was evaluated.

Results

The expression of EGFR and HER2 mRNA increased significantly after the experiment. However, it was higher after laparotomy than after CO₂ pneumoperitoneum at almost all examined time points. Survivin mRNA expression increased significantly in the first 48 h, then returned to the control level. It was higher after laparotomy than after CO₂ pneumoperitoneum 48 h after the surgical procedures.

Conclusion

The expression of EGFR, HER2, and survivin increased after each surgical procedure. However it was lower after CO₂ pneumoperitoneum than after laparotomy. This might be associated with changes in the chemosensitivity of the remnant cancer cells after surgery, supporting the use of minimally invasive surgery for cancer.

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Title: Increased mRNA expression of epidermal growth factor receptor, human epidermal receptor, and survivin in human gastric cancer after the surgical stress of laparotomy versus carbon dioxide pneumoperitoneum in a murine model
Authors: Anwar Tawfik Amin
Norio Shiraishi
Shigeo Ninomiya
Masaaki Tajima
Masafumi Inomata
Seigo Kitano
Publication date: 01-06-2010
Publisher: Springer-Verlag
Published in: Surgical Endoscopy / Issue 6/2010
Print ISSN: 0930-2794
Electronic ISSN: 1432-2218
DOI: https://doi.org/10.1007/s00464-009-0793-8

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Springer Medicine

Increased mRNA expression of epidermal growth factor receptor, human epidermal receptor, and survivin in human gastric cancer after the surgical stress of laparotomy versus carbon dioxide pneumoperitoneum in a murine model

Abstract

Background

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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