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Reproductive Biology and Endocrinology
Published in: Reproductive Biology and Endocrinology 1/2010

Open Access 01-12-2010 | Research

Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer

Authors: Suzie E Ahn, Jin Won Choi, Deivendran Rengaraj, Hee Won Seo, Whasun Lim, Jae Yong Han, Gwonhwa Song

Published in: Reproductive Biology and Endocrinology | Issue 1/2010

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Abstract

Background

Cysteine cathepsins (CTSs) are involved in the degradation and remodeling of the extracellular matrix and are associated with cell transformation, differentiation, motility, and adhesion. These functions are also related to cancer cell invasion and metastasis. Chickens spontaneously develop epithelial ovarian cancer and are therefore a good animal model for human ovarian cancer. However, no studies have investigated the expression of CTSs in chickens with ovarian cancer.

Methods

Cancerous (n = 5) and normal (n = 3) ovaries were collected from 2-to 3-year-old hens, and ovarian tissue samples were collected for study. Ovarian cancers were evaluated with hematoxylin and eosin staining. Reverse transcriptase and quantitative PCR analyses, in situ hybridization analysis were performed to examine the mRNA expression pattern of three CTSs in detail, and protein expression of CTSB was evaluated.

Results

The CTSB, CTSC, and CTSS genes were highly expressed in cancerous chicken ovaries. Messenger RNAs for the three CTSs were localized to a nodule area, a major characteristic of cancerous ovaries, but the three CTSs showed no specific localization in normal ovaries. Immunoreactive CTSB protein was present in the nodule area of cancerous ovaries.

Conclusion

Our results suggest that CTSB, CTSC, and CTSS have important functions in the development of epithelial ovarian cancer.
Appendix
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Metadata
Title
Increased expression of cysteine cathepsins in ovarian tissue from chickens with ovarian cancer
Authors
Suzie E Ahn
Jin Won Choi
Deivendran Rengaraj
Hee Won Seo
Whasun Lim
Jae Yong Han
Gwonhwa Song
Publication date
01-12-2010
Publisher
BioMed Central
Published in
Reproductive Biology and Endocrinology / Issue 1/2010
Electronic ISSN: 1477-7827
DOI
https://doi.org/10.1186/1477-7827-8-100

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