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01-04-2016 | Original Article

Increased endothelial microparticles and oxidative stress at extreme altitude

Authors: Jacqueline Pichler Hefti, Alexander Leichtle, Monika Stutz, Urs Hefti, Thomas Geiser, Andreas R. Huber, Tobias M. Merz

Published in: European Journal of Applied Physiology | Issue 4/2016

Abstract

Purpose

Hypoxia and oxidative stress affect endothelial function. Endothelial microparticles (MP) are established measures of endothelial dysfunction and influence vascular reactivity. To evaluate the effects of hypoxia and antioxidant supplementation on endothelial MP profiles, a double-blind, placebo-controlled trial, during a high altitude expedition was performed.

Methods

29 participants were randomly assigned to a treatment group (n = 14), receiving vitamin E, C, A, and N-acetylcysteine daily, and a control group (n = 15), receiving placebo. Blood samples were obtained at 490 m (baseline), 3530, 4590, and 6210 m. A sensitive tandem mass spectrometry method was used to measure 8-iso-prostaglandin F_2α and hydroxyoctadecadienoic acids as markers of oxidative stress. Assessment of MP profiles including endothelial activation markers (CD62+MP and CD144+MP) and cell apoptosis markers (phosphatidylserine+MP and CD31+MP) was performed using a standardized flow cytometry-based protocol.

Results

15 subjects reached all altitudes and were included in the final analysis. Oxidative stress increased significantly at altitude. No statistically significant changes were observed comparing baseline to altitude measurements of phosphatidylserine expressing MP (p = 0.1718) and CD31+MP (p = 0.1305). Compared to baseline measurements, a significant increase in CD62+MP (p = 0.0079) and of CD144+MP was detected (p = 0.0315) at high altitudes. No significant difference in any MP level or oxidative stress markers were found between the treatment and the control group.

Conclusion

Hypobaric hypoxia is associated with increased oxidative stress and induces a significant increase in CD62+ and CD144+MP, whereas phosphatidylserine+MP and CD31+MP remain unchanged. This indicates that endothelial activation rather than an apoptosis is the primary factor of hypoxia induced endothelial dysfunction.

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Title: Increased endothelial microparticles and oxidative stress at extreme altitude
Authors: Jacqueline Pichler Hefti
Alexander Leichtle
Monika Stutz
Urs Hefti
Thomas Geiser
Andreas R. Huber
Tobias M. Merz
Publication date: 01-04-2016
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 4/2016
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-015-3309-3

At a glance: The STEP trials

Springer Medicine

Increased endothelial microparticles and oxidative stress at extreme altitude

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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