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01-05-2013 | Original Article

Increased contractility and impaired relaxation of the left pulmonary artery in a rabbit model of congenital diaphragmatic hernia

Authors: Augusto Frederico Schmidt, Julio Alejandro Rojas-Moscoso, Frances Lilian Lanhellas Gonçalves, Rodrigo Melo Gallindo, Fabíola Zakia Mónica, Edson Antunes, Rebeca Lopes Figueira, Lourenço Sbragia

Published in: Pediatric Surgery International | Issue 5/2013

Abstract

Purpose

Congenital diaphragmatic hernia (CDH) is associated with pulmonary hypertension which is often difficult to manage and a significant cause of morbidity and mortality. Our aim was to study the pulmonary artery reactivity in an animal model of CDH.

Methods

To investigate the reactivity of the aorta and left pulmonary artery in a rabbit model of CDH, we studied the in vitro responses to the α₁-adrenoceptor agonist phenylephrine (PE) and to both the muscarinic receptor agonist (ACh) and the nitric oxide (NO) donor sodium nitroprusside (SNP). Rabbits underwent surgery at 25 days of gestation. CDH was created in one fetus per horn (n = 8). Remaining fetuses were considered controls (n = 18). At term (30 days), the lung, left pulmonary artery, and aorta were dissected. In a separate group, endothelium was mechanically removed.

Results

There were no differences in the contractile and relaxing responses of aorta in all groups. In left pulmonary artery, PE-induced contractions were significantly greater (p < 0.05) in CDH when compared with control group. The increased responsiveness to PE in CDH group was similar to that found in pulmonary artery without endothelium. The ACh-induced pulmonary artery relaxation was markedly reduced in CDH when compared with control group (p < 0.05), whereas no differences were found for SNP.

Conclusion

Our results show increased contractility and impairment in endothelium-dependent relaxation of pulmonary artery in CDH, mimicking an endothelial dysfunction, with preserved response to endothelium-independent mechanism.

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Title: Increased contractility and impaired relaxation of the left pulmonary artery in a rabbit model of congenital diaphragmatic hernia
Authors: Augusto Frederico Schmidt
Julio Alejandro Rojas-Moscoso
Frances Lilian Lanhellas Gonçalves
Rodrigo Melo Gallindo
Fabíola Zakia Mónica
Edson Antunes
Rebeca Lopes Figueira
Lourenço Sbragia
Publication date: 01-05-2013
Publisher: Springer-Verlag
Published in: Pediatric Surgery International / Issue 5/2013
Print ISSN: 0179-0358
Electronic ISSN: 1437-9813
DOI: https://doi.org/10.1007/s00383-012-3238-8

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Increased contractility and impaired relaxation of the left pulmonary artery in a rabbit model of congenital diaphragmatic hernia

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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