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01-06-2019 | Original Article

In vivo high-resolution diffusion tensor imaging of the developing neonatal rat cortex and its relationship to glial and dendritic maturation

Authors: Markus Breu, Dominik Reisinger, Liangcheng Tao, Dan Wu, Yajing Zhang, Matthew D. Budde, Ali Fatemi, Arvind P. Pathak, Jiangyang Zhang

Published in: Brain Structure and Function | Issue 5/2019

Abstract

Diffusion tensor imaging (DTI) is increasingly utilized as a sensitive tool for studying brain maturation and injuries during the neonatal period. In this study, we acquired high resolution in vivo DTI data from neonatal rat brains from postnatal day 2 (P2) to P10 and correlated temporal changes in DTI derived markers with microstructural organization of glia, axons, and dendrites during this critical period of brain development. Group average images showed dramatic temporal changes in brain morphology, fractional anisotropy (FA) and mean diffusivity (MD). Most cortical regions showed a monotonous decline in FA and an initial increase in MD from P2 to P8 that declined slightly by P10. Qualitative histology revealed rapid maturation of the glial and dendritic networks in the developing cortex. In the cingulate and motor cortex, the decreases in FA over time significantly correlated with structural anisotropy values computed from histological sections stained with glial and dendritic markers. However, in the sensory and visual cortex, other factors probably contributed to the observed decreases in FA. We did not observe any significant correlations between FA and structural anisotropy computed from the axonal histological marker.

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Title: In vivo high-resolution diffusion tensor imaging of the developing neonatal rat cortex and its relationship to glial and dendritic maturation
Authors: Markus Breu
Dominik Reisinger
Liangcheng Tao
Dan Wu
Yajing Zhang
Matthew D. Budde
Ali Fatemi
Arvind P. Pathak
Jiangyang Zhang
Publication date: 01-06-2019
Publisher: Springer Berlin Heidelberg
Published in: Brain Structure and Function / Issue 5/2019
Print ISSN: 1863-2653
Electronic ISSN: 1863-2661
DOI: https://doi.org/10.1007/s00429-019-01878-w

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In vivo high-resolution diffusion tensor imaging of the developing neonatal rat cortex and its relationship to glial and dendritic maturation

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