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International Journal of Colorectal Disease
Published in: International Journal of Colorectal Disease 7/2017

01-07-2017 | Original Article

In vivo and ex vivo sentinel node mapping does not identify the same lymph nodes in colon cancer

Authors: Helene Schou Andersen, Astrid Louise Bjørn Bennedsen, Stefan Kobbelgaard Burgdorf, Jens Ravn Eriksen, Susanne Eiholm, Anders Toxværd, Lene Buhl Riis, Jacob Rosenberg, Ismail Gögenur

Published in: International Journal of Colorectal Disease | Issue 7/2017

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Abstract

Introduction

Identification of lymph nodes and pathological analysis is crucial for the correct staging of colon cancer. Lymph nodes that drain directly from the tumor area are called “sentinel nodes” and are believed to be the first place for metastasis. The purpose of this study was to perform sentinel node mapping in vivo with indocyanine green and ex vivo with methylene blue in order to evaluate if the sentinel lymph nodes can be identified by both techniques.

Methods

Patients with colon cancer UICC stage I–III were included from two institutions in Denmark from February 2015 to January 2016. In vivo sentinel node mapping with indocyanine green during laparoscopy and ex vivo sentinel node mapping with methylene blue were performed in all patients.

Results

Twenty-nine patients were included. The in vivo sentinel node mapping was successful in 19 cases, and ex vivo sentinel node mapping was successful in 13 cases. In seven cases, no sentinel nodes were identified. A total of 51 sentinel nodes were identified, only one of these where identified by both techniques (2.0%). In vivo sentinel node mapping identified 32 sentinel nodes, while 20 sentinel nodes were identified by ex vivo sentinel node mapping. Lymph node metastases were found in 10 patients, and only two had metastases in a sentinel node.

Conclusion

Placing a deposit in relation to the tumor by indocyanine green in vivo or of methylene blue ex vivo could only identify sentinel lymph nodes in a small group of patients.
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Metadata
Title
In vivo and ex vivo sentinel node mapping does not identify the same lymph nodes in colon cancer
Authors
Helene Schou Andersen
Astrid Louise Bjørn Bennedsen
Stefan Kobbelgaard Burgdorf
Jens Ravn Eriksen
Susanne Eiholm
Anders Toxværd
Lene Buhl Riis
Jacob Rosenberg
Ismail Gögenur
Publication date
01-07-2017
Publisher
Springer Berlin Heidelberg
Published in
International Journal of Colorectal Disease / Issue 7/2017
Print ISSN: 0179-1958
Electronic ISSN: 1432-1262
DOI
https://doi.org/10.1007/s00384-017-2777-9

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