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European Archives of Paediatric Dentistry

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01-12-2014 | Original Scientific Article

In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts

Authors: S. N. Al-Haj Ali, S. H. Al-Jundi, D. J. Ditto

Published in: European Archives of Paediatric Dentistry | Issue 6/2014

Abstract

Aim

This was to define and compare the in vitro toxicity of grey MTA with that of white MTA on cultured human periodontal ligament (PDL) fibroblasts.

Methods

PDL cells were obtained from sound first permanent molars and cultured in Dulbecco’s Modified Eagle’s Medium. Cultures were subjected to different concentrations of grey and white MTA (0.5, 5, 50 and 500 µg/ml) for 24 h at 37 °C. Cells that were not exposed to grey or white MTA served as the negative control. In vitro toxicity was assessed using MTT assay.

Statistics

The results were compared using ANOVA and Tukey statistical tests (p < 0.05).

Results

White MTA presented higher in vitro toxicity than grey MTA. However, the differences were almost insignificant (p > 0.05).

Conclusion

Both colours of MTA are biocompatible since they were both able to preserve PDL fibroblasts for up to 24 h. MTA is as a promising alternative in pulpotomy of primary teeth.

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Title: In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts
Authors: S. N. Al-Haj Ali
S. H. Al-Jundi
D. J. Ditto
Publication date: 01-12-2014
Publisher: Springer Berlin Heidelberg
Published in: European Archives of Paediatric Dentistry / Issue 6/2014
Print ISSN: 1818-6300
Electronic ISSN: 1996-9805
DOI: https://doi.org/10.1007/s40368-014-0134-z

Keynote webinar | Spotlight on medication adherence

Springer Medicine

In vitro toxicity of grey MTA in comparison to white MTA on human periodontal ligament fibroblasts

Abstract

Aim

Methods

Statistics

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Aim

Methods

Statistics

Results

Conclusion

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