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01-07-2011 | Natural Resource Letter

In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells

Authors: Jyunichiro Takahashi, Gen Toshima, Yukie Matsumoto, Fumiko Kimura, Takanobu Kiuchi, Kentaro Hamada, Keishi Hata

Published in: Journal of Natural Medicines | Issue 3-4/2011

Abstract

We screened the antihyperlipidemic effects of seven edible plants by evaluation of the triglyceride (TG) and cholesterol profiles secreted from HepG2 cells. We found that the water- and ethanol-extracts of Brasenia schreberi at 100 μg/ml exhibited strong inhibitory activities against TG and cholesterol secretions from HepG2 cells stimulated with sodium oleate. Real-time RT-PCR analysis demonstrated that ethanol extract of B. schreberi (BSET) attenuated the expression of the sterol regulatory element binding protein-1c and -2, fatty acid synthase and HMG CoA synthase-1 genes, which are involved in lipid synthesis in hepatocyte/hepatoma cells. Furthermore, we studied the action of BSET on adipose tissue accumulation and serum parameters in mice fed a high-fat diet (HFD). BSET suppressed mesenteric and epididymal adipose tissue accumulation and normalized serum TG and glucose, but not cholesterol levels in HFD-fed mice.

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Title: In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells
Authors: Jyunichiro Takahashi
Gen Toshima
Yukie Matsumoto
Fumiko Kimura
Takanobu Kiuchi
Kentaro Hamada
Keishi Hata
Publication date: 01-07-2011
Publisher: Springer Japan
Published in: Journal of Natural Medicines / Issue 3-4/2011
Print ISSN: 1340-3443
Electronic ISSN: 1861-0293
DOI: https://doi.org/10.1007/s11418-011-0542-x

At a glance: The STEP trials

Springer Medicine

In vitro screening for antihyperlipidemic activities in foodstuffs by evaluating lipoprotein profiles secreted from human hepatoma cells

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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