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Molecular Imaging and Biology

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01-12-2015 | Research Article

In Vitro Evaluation of Gd³⁺-Anionic Linear Globular Dendrimer-Monoclonal Antibody: Potential Magnetic Resonance Imaging Contrast Agents for Prostate Cancer Cell Imaging

Authors: Mehdi Mirzaei, Bita Mehravi, Mehdi Shafiee Ardestani, Seyed Amir Mohsen Ziaee, Peyman Pourghasem

Published in: Molecular Imaging and Biology | Issue 6/2015

Abstract

Purpose

Early stage prostate cancer diagnosis is of high global interest. Magnetic resonance imaging (MRI) is a non-invasive modality for early cancer diagnosis, in particular for prostate cancer detection. The research aim is to synthesize a nanodendrimer and its conjugate with C595 monoclonal antibody (mAb C595), against prostate cancer, followed by its chelating with Gd³⁺.

Procedures

Anti-MUC-1 mAb C595 was conjugated to an anionic linear globular dendrimer (ALGDG2). The polyethylene glycol core and citric acid shell were synthesized followed by loading with Gd³⁺ to make novel contrast agents for functional MRI. The in vitro behavior and MRI parameters of the nanoconjugate were investigated performing several studies such as cell toxicity and TNF-alpha evaluations. The investigation of magnetic resonance imaging parameters indicated how well nanoconjugate performs in ¹H-NMR and ¹⁷O-NMR in vitro.

Results

Results showed a potential specific MRI activity by improving the swelling responses cell binding. The MTT (2-(4,5-dimethyl-2-thiazolyl)-3,5-diphenyl-2H-tetrazolium bromide) assay demonstrated that this contrast agent had significant cytotoxicity on prostate cancer cells.

Conclusions

These results showed that Gd³⁺-ALGDG₂-C595 is a potential prostate molecular imaging agent and could be considered as an ideal functional nanoprobe. Additionally, further investigations by clinical trials are in the pipeline.

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Title: In Vitro Evaluation of Gd3+-Anionic Linear Globular Dendrimer-Monoclonal Antibody: Potential Magnetic Resonance Imaging Contrast Agents for Prostate Cancer Cell Imaging
Authors: Mehdi Mirzaei
Bita Mehravi
Mehdi Shafiee Ardestani
Seyed Amir Mohsen Ziaee
Peyman Pourghasem
Publication date: 01-12-2015
Publisher: Springer US
Published in: Molecular Imaging and Biology / Issue 6/2015
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI: https://doi.org/10.1007/s11307-015-0841-9

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Springer Medicine

In Vitro Evaluation of Gd³⁺-Anionic Linear Globular Dendrimer-Monoclonal Antibody: Potential Magnetic Resonance Imaging Contrast Agents for Prostate Cancer Cell Imaging

Abstract

Purpose

Procedures

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Procedures

Results

Conclusions

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