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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2013

Open Access 01-12-2013 | Research article

In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects

Authors: Abeer E Abd El-Wahab, Doaa A Ghareeb, Eman EM Sarhan, Marwa M Abu-Serie, Maha A El Demellawy

Published in: BMC Complementary Medicine and Therapies | Issue 1/2013

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Abstract

Background

Berberis vulgaris is a well known plant with traditional herbal medical history. The aims of this study was to bioscreen and compare the in vitro biological activity (antioxidant, cholinergic, antidaibetic and the anticancer) of barberry crude extract and berberine active compound.

Methods

The effect of B. vulgaris extract and berberine chloride on cellular thiobarbituric acid reactive species (TBARS) formation, diphenyle–α-picrylhydrazyl (DPPH) oxidation, cellular nitric oxide (NO) radical scavenging capability, superoxide dismutase (SOD), glutathione peroxidase (GPx), acetylcholinesterase (AChE) and α-gulcosidase activities were spectrophotometrically determined. On the other hand, the effect of extract and berberine as anticancer was estimated on three different cell lines which were MCF-7, HepG-2, and Caco-2 cells by using neutral red uptake assay which compared with control normal cells (PBMC).

Results

Our results showed that barberry crude extract contains 0.6 mg berberine/mg crude extract. Barberry extract showed potent antioxidative capacity through decreasing TBARS, NO and the oxidation of DPPH that associated with GPx and SOD hyperactivation. Inhibitory effect of berberis crude extract on α-glucosidase was more potent than that of berberine chloride, while both had the same AChE inhibitory effect. Besides, different concentrations of both berberine chloride and barberry ethanolic extract showed to have no growth inhibitory effect on normal blood cells (PBMC). Otherwise, both berberine chloride and barberry ethanolic extract showed to have inhibitory effect on the growth of breast, liver and colon cancer cell lines (MCF7, HepG2 and CACO-2, respectively) at different incubation times starting from 24 hrs up to 72 hrs and the inhibitory effect increased with time in a dose dependant manner.

Conclusion

This work demonstrates the potential of the barberry crude extract and its active alkaloid, berberine, on suppressing lipid peroxidation, suggesting a promising use in the treatment of hepatic oxidative stress, Alzheimer and idiopathic male factor infertility. Beside, berberis vulgaris ethanolic extract is safe non-toxic extract as it was not inhibit the growth of PBMC that can induce cancer cell death that could return to its powerful antioxidant activity.
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Literature
1.
El-Sayed M, Ghareeb DA, Sarhan EM, Khalil AA: Therapeutic Bio-screening of the bioactive extracted ingredients of Berberis vulgaris, berberine. FPSB. 2011, 5: 63-68.
2.
Flora of New South Wales, Volume 1. Edited by: Harden GJ. 2000, Kensington: NSW University Press
3.
Grieve M: 1931. A Modern Herbal. 1994, London: Tiger Books International
4.
Arayne MS, Sultana N, Bahadur SS: The berberis story: Berberis vulgaris in therapeutics. Pakistan J Pharmacol Sci. 2007, 20 (1): 83-92.
5.
Zargari A: Medicinal Plants. 1983, Tehran: Tehran University Press, 1:68
6.
Chevallier A: The Encyclopedia of Medicinal Plants. 2001, St Leonards: Dorling Kindersley
7.
Souri EAG, Dehmobed-Sharifabadi A, Nazifi A, Farsam H: Antioxidant activity of sixty plants from Iran. Iranian J Pharm Res. 2004, 3: 55-59.
8.
Timothy CBN, Gregory S, Kelly ND: Berberine: therapeutic potential of an alkaloid found in several medicinal plants. Altern Med Rev. 1997, 2: 94-103.
9.
Kunwar RM, Nepal BK, Kshhetri HB, Rai SK, Bussmann RW: Ethnomedicine in Himalaya: a case study from Dolpa, Humla, Jumla and Mustang districts of Nepal. J Ethnobiol Ethnomed. 2006, 2: 27-10.1186/1746-4269-2-27.CrossRefPubMedPubMedCentral
10.
Fatehi-Hassanabad Z, Jafarzadeh M, Tarhini A, Fatehi M: The antihypertensive and vasodilator effects of aqueous extract from Berberis vulgaris fruit on hypertensive rats. Phytother Res. 2005, 19 (3): 222-225. 10.1002/ptr.1661.CrossRefPubMed
11.
Ivanovska N, Philipov S: Study on the anti-inflammatory action of Berberis vulgaris root extract, alkaloid fractions and pure alkaloids. Int J Immunopharmacol. 1996, 18 (10): 553-561. 10.1016/S0192-0561(96)00047-1.CrossRefPubMed
12.
Imanshahidi M, Hosseinzadeh H: Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, Berberine. Phytotherapy Research 2008. Published online at Wiley InterScience. Adv Physiol Educ. doi:10.1002/ptr.2399 (http://​www.​interscience.​wiley.​com)
13.
Mills S, Bone K: Principals and Practice of Phytotherapy. 2000, Edinburgh: Churchill Livingstone
14.
Bone K: A Clinical Guide to Blending Liquid Herbs: herbal formulations for the individual patient. 2003, St Louis, Missouri: Churchill Livingstone
15.
Li SY, TheBS LL, Seow WK, Thong YH: Anti-inflammatory and immuno-suppressive properties of the bis-benzylquinolones: in-vitro comparisons of tetrandrine and berbamine. Int J Immunopharmacol. 1989, 11: 395-441. 10.1016/0192-0561(89)90086-6.CrossRefPubMed
16.
Khosrokhavar R, Ahmadiani ASF: Antihistaminic and Anticholinergic Activity of Methanolic Extract of Barberry Fruit (Berberis vulgaris) in the Guinea- Pig Ileum. J Med Plants. 2010, 9: 99-105.
17.
Akhter MH: SMaBN: Possible mechanism of antidiarrheal effect of berberine. Indian J Med Res. 1979, 70: 233-241.PubMed
18.
Rabbani GHBT, Knight J, Sanyal SC, Alam K: Randomized controlled trial of berberine sulfate therapy for diarrhea due to enterotoxigenic Escherichia coli and Vibrio cholera. J Infect Dis. 1987, 155: 979-984. 10.1093/infdis/155.5.979.CrossRefPubMed
19.
Amin AH, Subbaiah TV, Abbasi KM: Berberine sulfate: antimidrobial activity, bioassay, and mode of action. Can J Microbiol. 1969, 15: 1067-1076. 10.1139/m69-190.CrossRefPubMed
20.
Leung AY, Foster S: Encyclopedia of Common Natural Ingredients Used in Food, Drugs and Cosmetics. 1996, New York: Wiley Publishers, 66-67.
21.
Imanshahidi M, Hosseinzadeh H: Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Phytother Res. 2008, 22: 999-1012. 10.1002/ptr.2399.CrossRefPubMed
22.
Sabir M: Study of some pharmacological actions of berberine. Indian J Physiol Pharmacol. 1971, 15: 111-132.PubMed
23.
Kuo C, Chi C, Liu T: The anti-inflammatory potential of berberine in-vitro and in-vivo. Cancer Lett. 2004, 203: 127-137. 10.1016/j.canlet.2003.09.002.CrossRefPubMed
24.
Zhou H, Mineshita S: The effect of berberine chloride on experimental colitis in rats in vivo and in vitro. J Pharmacol Exp Ther. 2000, 294 (3): 822-829.PubMed
25.
KF L: Berberine. Adverse Effects of Herbal Drugs. Edited by: Pagm KK, Hansel R, Chandler RF. 1994, Berlin: Springer Verlag, 97-98.
26.
Shamsa F, Ahmadiani A, Khosrokhavar R: Antihistaminic and anticholinergic activity of berberry fruit (Berberis vulgaris) in the guinea-pig ileum. J Ethnopharmacol. 1999, 64: 161-166. 10.1016/S0378-8741(98)00122-6.CrossRefPubMed
27.
Tomosaka H, Salim AA, Keller WJ, Chai H, Kinghorn AD: Antioxidant and cytoprotective compounds from Berberis vulgaris (barberry). Phytother Res. 2008, 22: 979-981. 10.1002/ptr.2443.CrossRefPubMed
28.
Cohen-Boulakia F: In-vivo sequential study of skeletal muscle capillary permeability in diabetic rats. Metabolism. 2000, 49: 880-885. 10.1053/meta.2000.6754.CrossRefPubMed
29.
Meiers S: The anthocyanidins cyaniding and delphinidin are potent inhibitors of the epidermal growth factor receptor. J Agric Food Chem. 2000, 49: 958-962.CrossRef
30.
31.
32.
Qadir S, Han J, Ji H, Chung H, Ahn J, Lee H: Effect of different extraction protocols on anticancer and antioxidant activities of berberis koreana bark extracts. J Biosci Bioeng. 2009, 107: 331-339. 10.1016/j.jbiosc.2008.11.021.CrossRefPubMed
33.
Ellman G, Andres V, Featherstone RA: New and rapid colorimetric determination of acetylcholinesterase activity. Biochem Pharmacol. 1961, 7: 88-95. 10.1016/0006-2952(61)90145-9.CrossRefPubMed
34.
Han YN, Srinivasan VR: Purification and characterization of beta-glucosidase of Alcaligenesfaecalis. J Bacteriol. 1969, 100: 1355-1363.PubMedPubMedCentral
35.
Wills ED: Mechanisms of lipid peroxide formation in animal tissues. Biochem J. 1965, 99 (3): 667-676.CrossRef
36.
Choi DW, King YC, Leroy P, Siest G, Wellman M: Differential role of CYP2E1 binders and isoniazid on CYP2E1 protein modification in NADPHdependent microsomal oxidative reactions: FR scavenging ability of isoniazid. Free Radic Res. 2002, 36: 893-904. 10.1080/1071576021000005339.CrossRefPubMed
37.
Green LC, Glogowski J: Analysis of nitrate, nitrite and nitrate in biological fluids. Anal Biochem. 1982, 126: 131-138. 10.1016/0003-2697(82)90118-X.CrossRefPubMed
38.
Marklund S, Marklund G: Involvement of the superoxide anion radical in the auto oxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem. 1974, 74: 469-474.CrossRef
39.
Paglia E, Valentine N: Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J Lab Clinic Med. 1967, 70: 158-169.
40.
Repetto G, Zurita J: Neutral red uptake assay for the estimation of cell viability/cytotoxicity. Nat Protoc. 2008, 3: 1125-1131. 10.1038/nprot.2008.75.CrossRefPubMed
41.
Wongbutdee J: physiological effects of berberine. Thai Pharm Health Sci J. 2008, 4: 78-83.
42.
Ghareeb D, Newarry A, El-Rashidy F, Hussein H, Ali A: Efficacy of natural extracts of Ginkgo biloba and barberry and a synthetic derivative of genistein (ipriflavone), as acetylcholinesterase inhibitors, comparative study with Aricept® effect. J Biochem Biotechnol. 2010, 1: 15-20.
43.
Muñoz-Torrero D: Acetylcholinesterase inhibitors as disease-modifying therapies for Alzheimer’s disease. Curr Med Chem. 2008, 15: 2433-2455. 10.2174/092986708785909067.CrossRefPubMed
44.
Yibchok-anun S, Jittaprasatsin W, Somtir D, Bunlunara W, Adisakwattana S: Insulin secreting and a-glucosidase inhibitory activity of Coscinium fenestratum and postprandial hyperglycemia in normal and diabetic rats. J Med Plants Res. 2009, 3 (9): 646-651.
45.
Bijvoet A, Pieper F, Vliet M, Boer H, Ploeg A, Verbeet M, Reuser A: Recombinant human acid α-glucosidase: high level production in mouse milk, biochemical characteristics, correction of enzyme deficiency in GSDII KO mice. Hum Mol Genet. 1998, 7: 1815-1824. 10.1093/hmg/7.11.1815.CrossRefPubMed
46.
Frei B: Natural antioxidants in human health and disease. 1994, San Diego: Academic Press
47.
Jagetia G, Baliga M, Babu K: The Evaluation of Nitric Oxide Scavenging Activity of Certain Herbal Formulations in vitro: A Preliminary Study. Phytother Res. 2004, 18: 561-565. 10.1002/ptr.1494.CrossRefPubMed
48.
Tan W, Huang M, Li Y, Chen M, Wu G, Gong J, Zhong Z, Xu Z, Dang Y, Guo J, Chen X, Wang Y: Anti-cancer natural products isolated from chinese medicinal herbs. Chin Med. 2011, 6: 27-43. 10.1186/1749-8546-6-27.CrossRefPubMedPubMedCentral
49.
Newman DJ, Snader KM: Natural products as sources of new drugs over the period 1981–2002. J Nat Prod. 2003, 66: 1022-1037. 10.1021/np030096l.CrossRefPubMed
50.
Ong ES, Woo SO, Yong YL: Pressurized liquid extraction of berberine and aristolochic acids in medicinal plants. J Chromatogr A. 2000, 904: 57-64. 10.1016/S0021-9673(00)00914-6.CrossRefPubMed
51.
Yoo KY, Hwang IK, Kim JD, Kang IJ, Park J, Yi J, Kim JK, Bae YS, Won MH: Antiinflammatory effect of the ethanol extract of Berberis koreana in a gerbil model of cerebral ischemia/reperfusion. Phytother Res. 2008, 22 (11): 1527-32. 10.1002/ptr.2527.CrossRefPubMed
52.
Yoo KY, Hwang IK, Lim BO, Kang TC, Kim DW, Kim SM, Lee HY, Kim JD, Won MH: Berberry extract reduces neuronal damage and N-Methyl-D-aspartate receptor 1 immunoreactivity in the gerbil hippocampus after transient forebrain ischemia. Biol Pharm Bull. 2006, 29 (4): 623-628. 10.1248/bpb.29.623.CrossRefPubMed
53.
Cho BJ, Im EK, Kwon JH, Lee KH, Shin HJ, Oh J, Kang SM, Chung JH, Jang Y: Berberine inhibits the production of lysophosphatidylcholine-induced reactive oxygen species and the ERK1/2 pathway in vascular smooth muscle cells. Mol Cells. 2005, 20 (3): 429-434.PubMed
54.
Musumeci R, Speciale A, Costanzo R, Annino A, Ragusa S, Rapisarda A, Pappalardo MS, Iauk L: Berberis aetnensis C. Presl. extracts: antimicrobial properties and interaction with ciprofloxacin. Int J Antimicrob Agents. 2003, 22 (1): 48-53. 10.1016/S0924-8579(03)00085-2.CrossRefPubMed
55.
Chung JG, Chen GW, Hung CF, Lee JH, Ho CC, Ho HC, Chang HL, Lin WC, Lin JG: Effects of berberine on arylamine N-acetyltransferase activity and 2-aminofluorene-DNA adduct formation in human leukemia cells. Am J Chin Med. 2000, 28 (2): 227-238. 10.1142/S0192415X00000271.CrossRefPubMed
56.
Ivanovska N, Philipov S, Hristova M: Influence of berberine on T-cell mediated immunity. Immunopharmacol Immunotoxicol. 1999, 21 (4): 771-86. 10.3109/08923979909007141.CrossRefPubMed
57.
Fukuda K, Hibiya Y, Mutoh M, Koshiji M, Akao S, Fujiwara H: Inhibition by berberine of cyclooxygenase-2 transcriptional activity in human colon cancer cells. J Ethnopharmacol. 1999, 66 (2): 227-33. 10.1016/S0378-8741(98)00162-7.CrossRefPubMed
58.
Mahata S, Bharti AC, Shukla S, Tyagi A, Husain SA, Das BC: Berberine modulates AP-1 activity to suppress HPV transcription and downstream signaling to induce growth arrest and apoptosis in cervical cancer cells. Mol Cancer. 2011, 10: 39-10.1186/1476-4598-10-39.CrossRefPubMedPubMedCentral
59.
Kim JB, Yu JH, Ko E, Lee KW, Song AK, Park SY, Shin I, Han W, Noh DY: The alkaloid Berberine inhibits the growth of Anoikis-resistant MCF-7 and MDA-MB-231 breast cancer cell lines by inducing cell cycle arrest. Phytomedicine. 2010, 17 (6): 436-740. 10.1016/j.phymed.2009.08.012.CrossRefPubMed
60.
Wang GY, Lv QH, Dong Q, Xu RZ, Dong QH: Berbamine induces Fas-mediated apoptosis in human hepatocellular carcinoma HepG2 cells and inhibits its tumor growth in nude mice. J Asian Nat Prod Res. 2009, 11 (3): 219-28. 10.1080/10286020802675076.CrossRefPubMed
61.
Ho YT, Yang JS, Lu CC, Chiang JH, Li TC, Lin JJ, Lai KC, Liao CL, Lin JG, Chung JG: Berberine inhibits human tongue squamous carcinoma cancer tumor growth in a murine xenograft model. Phytomedicine. 2009, 16 (9): 887-890. 10.1016/j.phymed.2009.02.015.CrossRefPubMed
62.
Hsieh YS, Kuo WH, Lin TW, Chang HR, Lin TH, Chen PN, Chu SC: Protective effects of berberine against low-density lipoprotein (LDL) oxidation and oxidized LDL-induced cytotoxicity on endothelial cells. J Agric Food Chem. 2007, 55 (25): 10437-10445. 10.1021/jf071868c.CrossRefPubMed
63.
Lin SS, Chung JG, Lin JP, Chuang JY, Chang WC, Wu JY, Tyan YS: Berberine inhibits arylamine N-acetyltransferase activity and gene expression in mouse leukemia L 1210 cells. Phytomedicine. 2005, 12 (5): 351-358. 10.1016/j.phymed.2003.11.008.CrossRefPubMed
64.
Fukuda K, Hibiya Y, Mutoh M, Koshiji M, Akao S, Fujiwara H: Inhibition of activator protein 1 activity by berberine in human hepatoma cells. Planta Med. 1999, 65 (4): 381-387. 10.1055/s-2006-960795.CrossRefPubMed
65.
Jenny M, Wondrak A, Zvetkova E, Tram N, Phiz P5, Schennach H, Culig Z, Ueberall F, Fuchs D: Crinum Latifolium Leave Extracts Suppress Immune Activation Cascades in Peripheral Blood Mononuclear Cells and Proliferation of Prostate Tumor Cells. Sci Pharm. 2011, 79: 323-335. 10.3797/scipharm.1011-13.CrossRefPubMedPubMedCentral
66.
Fotakis G, Timbrell A: In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride. Toxicol Lett. 2006, 160 (2): 171-177. 10.1016/j.toxlet.2005.07.001.CrossRefPubMed
67.
Kim T, Kang B, Cho D, Kim S: Induction of interleukin-12 production in mouse macrophages by berberine, a benzodioxoloquinolizine alkaloid, deviates CD4+ T cells from a Th2 to a Th1 response. Immunology. 2003, 109 (3): 407-414. 10.1046/j.1365-2567.2003.01673.x.CrossRefPubMedPubMedCentral
68.
Kenny T, Keen C, Schmitz H, Gershwin M: Immune Effects of Cocoa Procyanidin Oligomers on Peripheral Blood Mononuclear Cells. Exp Biol Med. 2007, 232: 293-300.
69.
Kashyap R, Husain A, Morey S, Panchbhai M, Deshpande P, Purohit H, Taori G, Daginawala H: Assessment of immune response to repeat stimulation with BCG vaccine using in vitro PBMC model. J Immune Based Ther Vaccin. 2010, 8: 3-10.1186/1476-8518-8-3.CrossRef
70.
71.
Metadata
Title
In vitro biological assessment of berberis vulgaris and its active constituent, berberine: antioxidants, anti-acetylcholinesterase, anti-diabetic and anticancer effects
Authors
Abeer E Abd El-Wahab
Doaa A Ghareeb
Eman EM Sarhan
Marwa M Abu-Serie
Maha A El Demellawy
Publication date
01-12-2013
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2013
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/1472-6882-13-218

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