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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2015

Open Access 01-12-2015 | Research article

In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis

Authors: Raquel Raick P. da Silva, Bruno J. M. da Silva, Ana Paula D. Rodrigues, Luis Henrique S. Farias, Milton N. da Silva, Danila Teresa V. Alves, Gilmara N. T. Bastos, José Luiz M. do Nascimento, Edilene O. Silva

Published in: BMC Complementary Medicine and Therapies | Issue 1/2015

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Abstract

Background

Leishmaniasis is an infectious disease caused by various species of the protozoan parasites of the Leishmania genus and transmitted by phlebotomine sandflies. The protozoa multiply in phagocytic cells, mainly macrophages, which play an important role defending the organism from pathogens. The most effective treatment for leishmaniasis is the chemotherapy and besides the high cost, these drugs are toxic and require a long period of treatment. Currently, some herbal products are considered an important alternative source of a new leishmanicidal agent, which includes the plant Physalis angulata, . We evaluated effects of an aqueous extract from roots of Physalis angulata (AEPa) on Leishmania proliferation, morphology and also determined whether physalins were present in the extract contributing to the knowledge of its pharmacological efficacy.

Methods

Morphological alterations were determined by light microscopy, transmission and scanning electron microscopy. Host cell viability was evaluated by MTT, and propidium iodide. AEPa were submitted in full HRESITOF analysis.

Results

AEPa promoted a dose-dependent reduction on promastigotes (IC50 = 39.5 μg/mL ± 5.1) and amastigotes (IC50 = 43.4 μg/mL ± 10.1) growth. This growth inhibition was associated with several morphological alterations observed in promastigote forms. No cytotoxic effect in mammalian cells was detected (IC50 > 4000 μg/mL). Furthemore, the presence of physalins A, B, D, E, F, G and H were described, for the first time, in the P. angulata root.

Conclusions

Results demonstrate that AEPa effectively promotes antileishmanial activity with several important morphological alterations and has no cytotoxic effects on host cells.
Appendix
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Literature
1.
2.
3.
Silveira FT, Lainson R, Corbett CEP. Clinical and Immunopathological Spectrum of American Cutaneous Leishmaniasis with special reference to the disease in Amazonian Brazil - A Review. Mem Inst Oswaldo Cruz. 2010;99:239–51.CrossRef
4.
Rosa MSS, Mendonça-Filho RR, Bizzo HR, Rodrigues IA, Soares RMA, Padrón TS, et al. Antileishmanial activity of a Linalool-Rich essential oil from Croton cajucara. Antimicrob Agents Chemother. 2003;47:1895–901.CrossRefPubMedCentral
5.
Luize PS, Tiuman TS, Morello LG, Maza PK, Ueda-Nakamura T, Filho BPD, et al. Effects of medicinal plant extracts on growth of Leishmania (L.) amazonensis and Trypanosoma cruzi. Braz J Pharm Sci. 2005;41:85–94.
6.
Laurella LC, Frank FM, Sarquiz A, Alonso MR, Giberti G, Cavallaro L, Catalán CA, Cazorla SI, Malchiodi E, Martino VS, Sülsem VP. In vitro evaluation of antiprotozoal and antiviral activities of extracts from Argentinean Mikania species. Sci W J. 2012;121253.
7.
Lusakibanza M, Mesia G, Tona G, Karemere S, Lukuka A, Tits M, Angenot L, Frédérich M. In vitro and in vivo antimalarial and cytotoxic activity of five plants used in congolese traditional medicine. J Ethnopharmacol. 2010;129: 398–402.
8.
Ruiz L, Ruiz L, Maco M, Cobos M, Gutierrez-Choquevilca AL, Roumy V. Plants used by native Amazonian groups from the Nanay River (Peru) for the treatment of malaria. J Ethnopharmacol. 2011;133:917–21.CrossRefPubMed
9.
Lin YS, Chiang HC, Kan WS, Hone E, Shih SJ, Won MH. Immunomodulatory activity of various fractions derived from Physalis angulata L. extract. Am J Chin Med. 1992;20:233–43.CrossRefPubMed
10.
Bastos GN, Santos AR, Ferreira VM, Costa AM, Bispo CI, Silveira AJ, et al. Antinociceptive effect of the aqueous extract obtained from roots of Physalis angulata L. on mice. J Ethnopharmacol. 2006;103:241–5.CrossRefPubMed
11.
Bastos GN, Silveira AJ, Salgado CG, Picanço-Diniz DL, Nascimento JL. Physalis angulata extract exerts anti-inflammatory effects in rats by inhibiting different pathways. J Ethnopharmacol. 2008;118:246–51.CrossRefPubMed
12.
Coelho-Ferreira M. Medicinal knowledge and plant utilization in an Amazonian coastal community of Marudá, Pará State (Brazil). J Ethnopharmacol. 2009;126:159–75.CrossRefPubMed
13.
Renjifo-Salgado E, Vargas-Arana G. Physalis angulata. L (Bolsa Mullaca): A review of its traditional uses, chemistry and pharmacology. Boletin Latino Americano Caribe Plant Med Aromat. 2013;12:431–45.
14.
Guimarães ET, Lima MS, Santos LA, Ribeiro IM, Tomassini TB, Dos Santos RR, et al. Activity of physalins purified from Physalis angulata in vitro and in vivo models of cutaneous leishmaniasis. J Antimicrob Chemother. 2009;64:84–7.CrossRefPubMed
15.
Guimarães ET, Lima MS, Santos LA, Ribeiro IM, Tomassini TB, Dos Santos RR, et al. Effects of seco-steroids purified from Physalis angulata L., Solanaceae, on the viability of Leishmania sp. Braz J Pharm. 2010;20:945–9.
16.
Sá MS, De Menezes MN, Krettli AU, Ribeiro IM, Tomassini TC, Ribeiro dos Santos R, et al. Antimalarial activity of physalins B, D, F, and G. J Nat Prod. 2011;74:2269–72.CrossRefPubMed
17.
Meira CS, Guimaraes ET, Bastos TB, Moreira DRM, Tomassini TCB, Ribeiro IM, et al. Physalins B and F, seco-steroids isolated from Physalis angulata L., strongly inhibit proliferation, ultrastructure and infectivity of Trypanosoma cruzi. Parasitol. 2013;140:1811–21.CrossRef
18.
Guimarães LR, Rodrigues AP, Marinho PS, Muller AH, Guilhon GM, Santos LS, et al. Activity of the julocrotine, a glutarimide alkaloid from Croton pullei var. glabrior, on Leishmania (L.) amazonensis. Parasitol Res. 2010;107:1075–81.CrossRefPubMed
19.
Rodrigues AP, Carvalho AS, Santos AS, Alves CN, Do Nascimento JL, Silva EO. Kojic acid, a secondary metabolite from Aspergillus sp. acts as an inducer of macrophage activation. Cell Biol Int. 2011;35:335–43.
20.
Garcia ES, Castro DP, Ribeiro IM, Tomassini TC, Azambuja P. Trypanosoma rangeli: effects of physalin B on the immune reactions of the infected larvae of Rhodnius prolixus. Exp Parasitol. 2006;112:37–43.CrossRefPubMed
21.
Silva BJM, Rodrigues APD, Farias LHS, Hage AAP, Nascimento JLM, Silva EO. Physalis angulata induces in vitro differentiation of murine bone marrow cells into macrophages. BMC Cell Biol. 2014;15:37.CrossRefPubMedPubMedCentral
22.
Delorenzi JC, Attias M, Gattass C, Andrade M, Rezende C, Pinto AC, et al. Antileishmanial activity of an indole alkaloid from Peschiera australis. Antimicrob Agents Chemother. 2001;45:1349–54.CrossRefPubMedPubMedCentral
23.
Vannier-Santos MA, De Castro SL. Electron microscopy in antiparasitic chemotherapy: a (close) view to a kill. Curr Drug Targets. 2009;10:246–60.CrossRefPubMed
24.
Adade CM, Souto-Padrón T. Contributions of ultrastructural studies to the cell biology of trypanosomatids: targets for anti-parasitic drugs. Open Parasitol J. 2010;4:178–87.CrossRef
25.
Duran G, Duran N, Culha G, Ozcan B, Oztas H, Ozer B. In vitro antileishmanial activity of Adana propolis samples on Leishmania tropica: a preliminary study. Parasitol Res. 2008;102:1217–25.CrossRefPubMed
26.
Santin MR, Dos Santos AO, Nakamura CV, Dias Filho BP, Ferreira IC, Ueda-Nakamura T. In vitro activity of the essential oil of Cymbopogon citratus and its major component (citral) on Leishmania amazonensis. Parasitol Res. 2009;105:1489–96.CrossRefPubMed
27.
28.
Wheeler RJ, Gluenz E, Gull K. The cell cycle of Leishmania: morphogenetic events and their implications for parasite biology. Mol Microbiol. 2011;79:647–62.CrossRefPubMed
29.
Oliveira VC, Moura DM, Lopes JA, De Andrade PP, Da Silva NH, Figueiredo RC. Effects of essential oils from Cymbopogon citratus (DC) Stapf., Lippia sidoides Cham., and Ocimum gratissimum L. on growth and ultrastructure of Leishmania chagasi promastigotes. Parasitol Res. 2009;104:1053–9.CrossRefPubMed
30.
De Souza W. An introduction to the structural organization of parasitic protozoa. Curr Pharm Des. 2008;14:822–38.CrossRefPubMed
31.
Vendrametto MC, Santos AO, Nakamura CV, Filho BPD, Cortez DAG, Ueda-Nakamura T. Evaluation of antileishmanial activity of eupomatenoid-5, a 19 compound isolated from leaves of Piper regnellii var. pallescens. Parasitol Int. 2010;59:154–8.CrossRefPubMed
32.
Rodrigues JCF, Attias M, Rodriguez C, Urbina JA, Souza W. Ultrastructural and biochemical alterations induced by 22, 26-azasterol, a Δ24(25)-sterol methyltransferase inhibitor, on promastigote and amastigote forms of Leishmania amazonensis. Antimicrob Agents Chemother. 2002;46:487–99.CrossRefPubMedPubMedCentral
33.
Santa-Rita RM, Henriques-Pons A, Barbosa HS, Castro SL. Effect of the lysophospholipid analogues edelfosine, ilmofosine and miltefosine against Leishmania amazonensis. J Antimicrob Chemother. 2004;54:704–10.CrossRefPubMed
34.
Ueda-Nakamura T, Mendonça-Filho RR, Morgado-Díaz JA, Maza PK, Dias Filho BP, Cortez AGD, et al. Antileishmanial activity of Eugenol-rich essential oil from Ocimum gratissimum. Parasitol Int. 2006;55:99–105.CrossRefPubMed
35.
Sen R, Chatterjee M. Plant derived therapeutics for the treatment of Leishmaniasis. Phytomed. 2011;18:1056–69.CrossRef
Metadata
Title
In vitro biological action of aqueous extract from roots of Physalis angulata against Leishmania (Leishmania) amazonensis
Authors
Raquel Raick P. da Silva
Bruno J. M. da Silva
Ana Paula D. Rodrigues
Luis Henrique S. Farias
Milton N. da Silva
Danila Teresa V. Alves
Gilmara N. T. Bastos
José Luiz M. do Nascimento
Edilene O. Silva
Publication date
01-12-2015
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2015
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-015-0717-1

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