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BMC Complementary Medicine and Therapies

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Open Access 01-12-2021 | Research article

In vitro antibacterial activity of Loxostylis alata extracts and isolated compounds against Salmonella species

Authors: Dorcas A. Gado, Muna Ali Abdalla, Aroke S. Ahmed, Balungile Madikizela, Sanah M. Nkadimeng, Marthie M. Ehlers, Lyndy J. McGaw

Published in: BMC Complementary Medicine and Therapies | Issue 1/2021

Abstract

Background

Owing to antibiotic resistance, alternative antimicrobials from medicinal plants are receiving attention as leads for anti-infective agents. This study aimed to investigate selected tree species and their constituents for activity against bacterial foodborne pathogens, particularly Salmonella serovars.

Methods

Antibacterial activity of ten plant species was determined by serial microdilution against bacteria implicated in causing gastrointestinal ailments. Active compounds were isolated from Loxostylis alata using bioassay-guided fractionation. Antioxidant activity was determined using free-radical scavenging assays. Cytotoxicity and genotoxicity of the extracts was ascertained on Vero cells, and using the Ames assay respectively.

Results

Extracts had low to moderate MIC values from 0.04 to 2.5 mg/mL. Protorhus longifolia and Loxostylis alata were most active and L. alata had the highest selectivity index value (2.51) against Salmonella Typhimurium, as well as high antioxidant activity. Cytotoxicity values ranged from 0.02 to 0.47 mg/mL, while tested extracts were not genotoxic. Bioactive compounds isolated from L. alata included delicaflavone and a polymethoxyflavone.

Conclusions

The Loxostylis alata leaf extract had strong activity against Salmonella serovars but isolated compounds were less active, indicating likely synergistic effects. Extracts of L. alata are promising candidates for development of antimicrobial preparations or food additives against microbial contamination.

Negi PS. Plant extracts for the control of bacterial growth: efficacy, stability and safety issues for food application. Int J Food Microbiol. 2012;156(1):7–17. https://doi.org/10.1016/j.ijfoodmicro.2012.03.006.CrossRefPubMed

WHO. Food safety fact sheet 2018. updated 31 October 2017. Available from: http://www.who.int/news-room/fact-sheets/detail/food-safety.

Carrasco E, Morales-Rueda A, García-Gimeno RM. Cross-contamination and recontamination by Salmonella in foods: a review. Food Res Int. 2012;45(2):545–56. https://doi.org/10.1016/j.foodres.2011.11.004.CrossRef

Boyle EC, Bishop JL, Grassl GA, Finlay BB. Salmonella: from pathogenesis to therapeutics. J Bacteriol. 2007;189(5):1489–95. https://doi.org/10.1128/JB.01730-06.CrossRefPubMed

Mahlangu ZP, Botha FS, Madoroba E, Chokoe K, Elgorashi EE. Antimicrobial activity of Albizia gummifera (J.F.Gmel.) C.A.Sm leaf extracts against four Salmonella serovars. S Afr J Bot. 2017;108:132–6.CrossRef

Barua H, Biswas PK, Olsen KEP, Shil SK, Christensen JP. Molecular characterization of motile serovars of Salmonella enterica from breeder and commercial broiler poultry farms in Bangladesh. PLoS One. 2013;8(3):e57811. https://doi.org/10.1371/journal.pone.0057811.CrossRefPubMedPubMedCentral

Feasey NA, Dougan G, Kingsley RA, Heyderman RS, Gordon MA. Invasive non-typhoidal salmonella disease: an emerging and neglected tropical disease in Africa. Lancet. 2012;379(9835):2489–99. https://doi.org/10.1016/S0140-6736(11)61752-2.CrossRefPubMedPubMedCentral

Feasey NA, Hadfield J, Keddy KH, Dallman TJ, Jacobs J, Deng X, et al. Distinct Salmonella Enteritidis lineages associated with enterocolitis in high-income settings and invasive disease in low-income settings. Nat Genet. 2016;48(10):1211–7.CrossRef

Bruun T, Sørensen G, Forshell LP, Jensen T, Nygård K, Kapperud G, et al. An outbreak of salmonella typhimurium infections in Denmark, Norway and Sweden, 2008. Euro Surveill (Online Edition). 2009;14(10):19147.

10.

Phillips I, Casewell M, Cox T, De Groot B, Friis C, Jones R, et al. Does the use of antibiotics in food animals pose a risk to human health? A critical review of published data. J Antimicrob Chemother. 2004;53(1):28–52. https://doi.org/10.1093/jac/dkg483.CrossRefPubMed

11.

Sarmah AK, Meyer MT, Boxall ABA. A global perspective on the use, sales, exposure pathways, occurrence, fate and effects of veterinary antibiotics (VAs) in the environment. Chemosphere. 2006;65(5):725–59. https://doi.org/10.1016/j.chemosphere.2006.03.026.CrossRefPubMed

12.

Kuppusamy S, Kakarla D, Venkateswarlu K, Megharaj M, Yoon Y-E, Lee YB. Veterinary antibiotics (VAs) contamination as a global agro-ecological issue: a critical view. Agric Ecosyst Environ. 2018;257:47–59. https://doi.org/10.1016/j.agee.2018.01.026.CrossRef

13.

Cooke FJ, Wain J, Mastroeni P, Maskell D. Antibiotic resistance in Salmonella infections. In: Salmonella infections: clinical, immunological and molecular aspects; 2006. p. 25–56. https://doi.org/10.1017/CBO9780511525360.003.CrossRef

14.

Ventola CL. The antibiotic resistance crisis: part 1: causes and threats. Pharm Ther. 2015;40(4):277–83.

15.

Threlfall EJ. Salmonella. In: Heggenhougen HK, editor. International encyclopedia of public health. Oxford: Academic Press; 2008. p. 639–47. https://doi.org/10.1016/B978-012373960-5.00613-4.CrossRef

16.

WHO. Antibiotic resistance factsheet 2018. updated 5 February 2018. Available from: http://www.who.int/news-room/fact-sheets/detail/antibiotic-resistance.

17.

Hemaiswarya S, Kruthiventi AK, Doble M. Synergism between natural products and antibiotics against infectious diseases. Phytomedicine. 2008;15(8):639–52. https://doi.org/10.1016/j.phymed.2008.06.008.CrossRefPubMed

18.

Koma OS, Fatokun OA, Theophilus OA. Phytochemical screening and in vitro antimicrobial activity of Waltheria Indica Linn leaf extracts. Biomed Sci. 2017;3(5):86. https://doi.org/10.11648/j.bs.20170305.11.CrossRef

19.

Spellberg B, Powers JH, Brass EP, Miller LG, Edwards JJE. Trends in antimicrobial drug development: implications for the future. Clin Infect Dis. 2004;38(9):1279–86. https://doi.org/10.1086/420937.CrossRefPubMed

20.

Eloff JN, McGaw LJ. Using African plant biodiversity to combat microbial infections. In: Novel Plant Bioresources: Applications in Food, Medicine and Cosmetics; 2014. p. 163–73. https://doi.org/10.1002/9781118460566.ch12.CrossRef

21.

Luepke KH, Suda KJ, Boucher H, Russo RL, Bonney MW, Hunt TD, et al. Past, present, and future of antibacterial economics: increasing bacterial resistance, limited antibiotic pipeline, and societal implications. Pharmacotherapy. 2017;37(1):71–84. https://doi.org/10.1002/phar.1868.CrossRefPubMed

22.

Pye CR, Bertin MJ, Lokey RS, Gerwick WH, Linington RG. Retrospective analysis of natural products provides insights for future discovery trends. Proc Natl Acad Sci. 2017;114(22):5601–6. https://doi.org/10.1073/pnas.1614680114.CrossRefPubMed

23.

Fromage G. Antibiotic resistance: an exploration of its causes and management strategies. J Aesthet Nurs. 2018;7(1):18–23. https://doi.org/10.12968/joan.2018.7.1.18.CrossRef

24.

Newman DJ, Cragg GM, Snader KM. The influence of natural products upon drug discovery. Nat Prod Rep. 2000;17(3):215–34. https://doi.org/10.1039/a902202c.CrossRefPubMed

25.

Cragg GM, Newman DJ. Natural products: a continuing source of novel drug leads. Biochim Biophys Acta Gen Subj. 2013;1830(6):3670–95.CrossRef

26.

Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod. 2016;79(3):629–61. https://doi.org/10.1021/acs.jnatprod.5b01055.CrossRefPubMed

27.

Ripa FA, Haque M, Imran-Ul-Haque M. In vitro antimicrobial, cytotoxic and antioxidant activity of flower extract of Saccharum spontaneum Linn. Eur J Sci Res. 2009;30(3):478–83.

28.

Elisha IL, Botha FS, McGaw LJ, Eloff JN. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med. 2017;17(1):133. https://doi.org/10.1186/s12906-017-1645-z.CrossRefPubMedPubMedCentral

29.

Van Wyk B-E, Wink M. Medicinal plants of the world: an illustrated scientific guide to important medicinal plants and their uses: timber press; 2004.

30.

Bozin B, Mimica-Dukic N, Samojlik I, Goran A, Igic R. Phenolics as antioxidants in garlic (Allium sativum L., Alliaceae). Food Chem. 2008;111(4):925–9. https://doi.org/10.1016/j.foodchem.2008.04.071.CrossRef

31.

Kumari P, Khatkar BS. Assessment of total polyphenols, antioxidants and antimicrobial properties of aonla varieties. J Food Sci Technol. 2016;53(7):3093–103. https://doi.org/10.1007/s13197-016-2282-0.CrossRefPubMedPubMedCentral

32.

Pauw E, Eloff JN. Which tree orders in southern Africa have the highest antimicrobial activity and selectivity against bacterial and fungal pathogens of animals? BMC Complement Altern Med. 2014;14(1):1.CrossRef

33.

Papuc C, Goran GV, Predescu CN, Nicorescu V, Stefan G. Plant polyphenols as antioxidant and antibacterial agents for shelf-life extension of meat and meat products: classification, structures, sources, and action mechanisms. Compr Rev Food Sci Food Saf. 2017;16(6):1243–68. https://doi.org/10.1111/1541-4337.12298.CrossRefPubMed

34.

Takó M, Kerekes EB, Zambrano C, Kotogán A, Papp T, Krisch J, et al. Plant Phenolics and phenolic-enriched extracts as antimicrobial agents against food-contaminating microorganisms. Antioxidants. 2020;9(2):165. https://doi.org/10.3390/antiox9020165.CrossRefPubMedCentral

35.

Jambalang AR, Buys EM, Botha FS. Bacterial species from retailed poultry eggs in Tshwane, South Africa: implication for consumers. S Afr J Sci. 2017;113(11–12):1–7. https://doi.org/10.17159/sajs.2017/20160232.CrossRef

36.

Eloff JN. A sensitive and quick microplate method to determine the minimal inhibitory concentration of plant extracts for bacteria. Planta Med. 1998;64(8):711–3. https://doi.org/10.1055/s-2006-957563.CrossRefPubMed

37.

Eloff JN. On expressing the antibacterial activity of plant extracts--a small first step in applying scientific knowledge to rural primary health care. S Afr J Sci. 2000;96(3):116–8.

38.

Dzoyem JP, McGaw LJ, Eloff JN. In vitro antibacterial, antioxidant and cytotoxic activity of acetone leaf extracts of nine under-investigated Fabaceae tree species leads to potentially useful extracts in animal health and productivity. BMC Complement Altern Med. 2014;14(1):1.CrossRef

39.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9–10):1231–7. https://doi.org/10.1016/S0891-5849(98)00315-3.CrossRefPubMed

40.

Brand-Williams W, Cuvelier M-E, Berset C. Use of a free radical method to evaluate antioxidant activity. LWT-Food Sci Technol. 1995;28(1):25–30. https://doi.org/10.1016/S0023-6438(95)80008-5.CrossRef

41.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65(1–2):55–63. https://doi.org/10.1016/0022-1759(83)90303-4.CrossRefPubMed

42.

McGaw LJ, Steenkamp V, Eloff JN. Evaluation of Athrixia bush tea for cytotoxicity, antioxidant activity, caffeine content and presence of pyrrolizidine alkaloids. J Ethnopharmacol. 2007;110(1):16–22. https://doi.org/10.1016/j.jep.2006.08.029.CrossRefPubMed

43.

Verschaeve L, Van Staden J. Mutagenic and antimutagenic properties of extracts from south African traditional medicinal plants. J Ethnopharmacol. 2008;119(3):575–87. https://doi.org/10.1016/j.jep.2008.06.007.CrossRefPubMed

44.

Madikizela B, McGaw LJ. Scientific rationale for traditional use of plants to treat tuberculosis in the eastern region of the OR Tambo district, South Africa. J Ethnopharmacol. 2018;224:250–60. https://doi.org/10.1016/j.jep.2018.06.002.CrossRefPubMed

45.

Efferth T, Kuete V. Cameroonian medicinal plants: pharmacology and derived natural products. Front Pharmacol. 2010;1:123.PubMedPubMedCentral

46.

Holetz FB, Pessini GL, Sanches NR, Cortez DAG, Nakamura CV, Dias Filho BP. Screening of some plants used in the Brazilian folk medicine for the treatment of infectious diseases. Mem Inst Oswaldo Cruz. 2002;97(7):1027–31. https://doi.org/10.1590/S0074-02762002000700017.CrossRefPubMed

47.

Suleiman MM, McGaw LJ, Naidoo V, Eloff JN. Evaluation of several tree species for activity against the animal fungal pathogen Aspergillus fumigatus. S Afr J Bot. 2010;76(1):64–71. https://doi.org/10.1016/j.sajb.2009.07.001.CrossRef

48.

Rios JL, Recio MC. Medicinal plants and antimicrobial activity. J Ethnopharmacol. 2005;100(1):80–4. https://doi.org/10.1016/j.jep.2005.04.025.CrossRefPubMed

49.

Ahmed AS, McGaw LJ, Elgorashi EE, Naidoo V, Eloff JN. Polarity of extracts and fractions of four Combretum (Combretaceae) species used to treat infections and gastrointestinal disorders in southern African traditional medicine has a major effect on different relevant in vitro activities. J Ethnopharmacol. 2014;154(2):339–50. https://doi.org/10.1016/j.jep.2014.03.030.CrossRefPubMed

50.

Gutiérrez D, Delgado S, Vázquez-Sánchez D, Martínez B, Cabo ML, Rodríguez A, et al. Incidence of Staphylococcus aureus and analysis of bacterial-associated communities on food industry surfaces. Appl Environ Microbiol. 2012;78(24):8547–54. AEM. 02045-12.

51.

National Center for Biotechnology Information. PubChem Compound Database; 1-Hexadecene: National Center for Biotechnology Information; 2005. Available from: https://pubchem.ncbi.nlm.nih.gov/compound/12395. Cited Dec 9, 2018

52.

Pooley E. Complete field guide to trees of Natal. Zululand & Transkei: Natal Flora Publications Trust; 1993.

53.

Pell SK. Molecular systematics of the cashew family (Anacardiaceae); 2004.

54.

Drewes SE, Horn MM, Mabaso NJ. Loxostylis alata and Smodingium argutum-a case of phytochemical bedfellows? S Afr J Bot. 1998;64(2):128–9. https://doi.org/10.1016/S0254-6299(15)30846-2.CrossRef

55.

Suleiman MM. The in vitro and in vivo biological activities of antifungal compounds isolated from Loxostylis alata ASprengex Rchbleaf extracts: University of Pretoria Repository; 2009.

56.

Aravind SG, Arimboor R, Rangan M, Madhavan SN, Arumughan C. Semi-preparative HPLC preparation and HPTLC quantification of tetrahydroamentoflavone as marker in Semecarpus anacardium and its polyherbal formulations. J Pharm Biomed Anal. 2008;48(3):808–13. https://doi.org/10.1016/j.jpba.2008.08.008.CrossRefPubMed

57.

Li S, Yao H, Zhao M, Li Y, Huang L, Lin X. Determination of seven biflavones of Selaginella doederleinii by high performance liquid chromatography. Anal Lett. 2013;46(18):2835–45. https://doi.org/10.1080/00032719.2013.831426.CrossRef

58.

Kang DG, Yin MH, Oh H, Lee DH, Lee HS. Vasorelaxation by amentoflavone isolated from Selaginella tamariscina. Planta Med. 2004;70(08):718–22. https://doi.org/10.1055/s-2004-827201.CrossRefPubMed

59.

Jung HJ, Sung WS, Yeo S-H, Kim HS, Lee I-S, Woo E-R, et al. Antifungal effect of amentoflavone derived fromSelaginella tamariscina. Arch Pharm Res. 2006;29(9):746–51. https://doi.org/10.1007/BF02974074.CrossRefPubMed

60.

Lee N-Y, Min H-Y, Lee J, Nam J-W, Lee Y-J, Han A-R, et al. Identification of a new cytotoxic biflavanone from Selaginella doederleinii. Chem Pharm Bull. 2008;56(9):1360–1. https://doi.org/10.1248/cpb.56.1360.CrossRef

61.

Russo A, Acquaviva R, Campisi A, Sorrenti V, Di Giacomo C, Virgata G, et al. Bioflavonoids as antiradicals, antioxidants and DNA cleavage protectors. Cell Biol Toxicol. 2000;16(2):91–8. https://doi.org/10.1023/A:1007685909018.CrossRefPubMed

62.

Waterman MJ, Nugraha AS, Hendra R, Ball GE, Robinson SA, Keller PA. Antarctic Moss Biflavonoids show high antioxidant and ultraviolet-screening activity. J Nat Prod. 2017;80(8):2224–31. https://doi.org/10.1021/acs.jnatprod.7b00085.CrossRefPubMed

63.

Sui Y, Yao H, Li S, Jin L, Shi P, Li Z, et al. Delicaflavone induces autophagic cell death in lung cancer via Akt/mTOR/p70S6K signaling pathway. J Mol Med. 2017;95(3):311–22. https://doi.org/10.1007/s00109-016-1487-z.CrossRefPubMed

64.

Lin L-C, Chou C-J. Three new biflavonoids from Selaginella delicatula. Chin Pharm J. 2000;52(4):211–8.

65.

Chen B, Wang X, Zou Y, Chen W, Wang G, Yao W, et al. Simultaneous quantification of five biflavonoids in rat plasma by LC-ESI–MS/MS and its application to a comparatively pharmacokinetic study of Selaginella doederleinii Hieron extract in rats. J Pharm Biomed Anal. 2018;149:80–8. https://doi.org/10.1016/j.jpba.2017.10.028.CrossRefPubMed

66.

Li S, Pan M-H, Lai C-S, Lo C-Y, Dushenkov S, Ho C-T. Isolation and syntheses of polymethoxyflavones and hydroxylated polymethoxyflavones as inhibitors of HL-60 cell lines. Bioorg Med Chem. 2007;15(10):3381–9. https://doi.org/10.1016/j.bmc.2007.03.021.CrossRefPubMed

67.

Murakami A, Nakamura Y, Torikai K, Tanaka T, Koshiba T, Koshimizu K, et al. Inhibitory effect of citrus nobiletin on phorbol ester-induced skin inflammation, oxidative stress, and tumor promotion in mice. Cancer Res. 2000;60(18):5059–66.PubMed

68.

Lin N, Sato T, Takayama Y, Mimaki Y, Sashida Y, Yano M, et al. Novel anti-inflammatory actions of nobiletin, a citrus polymethoxy flavonoid, on human synovial fibroblasts and mouse macrophages. Biochem Pharmacol. 2003;65(12):2065–71. https://doi.org/10.1016/S0006-2952(03)00203-X.CrossRefPubMed

69.

Ohguchi K, Iinuma M, Nozawa Y, Ito M. Nobiletin, a polymethoxylated flavone from citrus peels, induces differentiation of normal human epidermal keratinocytes. J Med Plants Res. 2014;8(33):1060–4.CrossRef

70.

Fan W-W, Yuan G-Q, Li Q-Q, Lin W. Antibacterial mechanisms of methyl gallate against Ralstonia solanacearum. Australas Plant Pathol. 2014;43(1):1–7. https://doi.org/10.1007/s13313-013-0234-y.CrossRef

71.

Anzoise ML, Basso AR, Del Mauro JS, Carranza A, Ordieres GL, Gorzalczany S. Potential usefulness of methyl gallate in the treatment of experimental colitis. Inflammopharmacology. 2018;26(3):839–49.

72.

Somani SJ, Modi KP, Majumdar AS, Sadarani BN. Phytochemicals and their potential usefulness in inflammatory bowel disease. Phytother Res. 2015;29(3):339–50. https://doi.org/10.1002/ptr.5271.CrossRefPubMed

73.

Teke GN, Lunga PK, Wabo HK, Kuiate J-R, Vilarem G, Giacinti G, et al. Antimicrobial and antioxidant properties of methanol extract, fractions and compounds from the stem bark of Entada abyssinica Stend ex A. Satabie. BMC Complement Altern Med. 2011;11(1):57.CrossRef

74.

Moharram FA-e, Al-Gendy AA, El-Shenawy SM, Ibrahim BM, Zarka MA. Phenolic profile, anti-inflammatory, antinociceptive, anti-ulcerogenic and hepatoprotective activities of Pimenta racemosa leaves. BMC Complement Altern Med. 2018;18(1):208.CrossRef

75.

Niehaus A, Apalata T, Coovadia Y, Smith A, Moodley P. An Outbreak of Foodborne Salmonellosis in Rural KwaZulu-Natal, South Africa; 2011. p. 693–7.

76.

Asnaashari M, Farhoosh R, Sharif A. Antioxidant activity of gallic acid and methyl gallate in triacylglycerols of Kilka fish oil and its oil-in-water emulsion. Food Chem. 2014;159:439–44. https://doi.org/10.1016/j.foodchem.2014.03.038.CrossRefPubMed

77.

Claudia D, Mario C-H, Arturo N, Omar Noel M-C, Antonio N, Teresa R, et al. Phenolic Compounds in Organic and Aqueous Extracts from Acacia farnesiana Pods Analyzed by ULPS-ESI-Q-oa/TOF-MS. In Vitro Antioxidant Activity and Anti-Inflammatory Response in CD-1 Mice. Molecules. 2018;23(9):2386.CrossRef

78.

Chae H-S, Kang O-H, Choi J-G, Oh Y-C, Lee Y-S, Brice O-O, et al. Methyl gallate inhibits the production of interleukin-6 and nitric oxide via down-regulation of extracellular-signal regulated protein kinase in RAW 264.7 cells. Am J Chin Med. 2010;38(05):973–83. https://doi.org/10.1142/S0192415X10008391.CrossRefPubMed

79.

Correa LB, Pádua TA, Seito LN, Costa TEMM, Silva MA, Candéa ALP, et al. Anti-inflammatory effect of methyl gallate on experimental arthritis: inhibition of neutrophil recruitment, production of inflammatory mediators, and activation of macrophages. J Nat Prod. 2016;79(6):1554–66. https://doi.org/10.1021/acs.jnatprod.5b01115.CrossRefPubMed

80.

Reyes A, Kim D, Simborio H, Hop H, Arayan L, Min W, et al. Methyl gallate limits infection in mice challenged with B rucella abortus while enhancing the inflammatory response. J Appl Microbiol. 2016;120(3):552–9. https://doi.org/10.1111/jam.13019.CrossRefPubMed

81.

Kang M-S, Oh J-S, Kang I-C, Hong S-J, Choi C-H. Inhibitory effect of methyl gallate and gallic acid on oral bacteria. J Microbiol. 2008;46(6):744–50. https://doi.org/10.1007/s12275-008-0235-7.CrossRefPubMed

82.

Birhanu BT, Park N-H, Lee S-J, Hossain MA, Park S-C. Inhibition of Salmonella Typhimurium adhesion, invasion, and intracellular survival via treatment with methyl gallate alone and in combination with marbofloxacin. Vet Res. 2018;49(1):101. https://doi.org/10.1186/s13567-018-0597-8.CrossRefPubMedPubMedCentral

83.

Lee S-H, Kim JK, Kim DW, Hwang HS, Eum WS, Park J, et al. Antitumor activity of methyl gallate by inhibition of focal adhesion formation and Akt phosphorylation in glioma cells. Biochim Biophys Acta Gen Subj. 2013;1830(8):4017–29.CrossRef

84.

Chaudhuri D, Ghate NB, Singh SS, Mandal N. Methyl gallate isolated from Spondias pinnata exhibits anticancer activity against human glioblastoma by induction of apoptosis and sustained extracellular signal-regulated kinase 1/2 activation. Pharmacogn Mag. 2015;11(42):269–76. https://doi.org/10.4103/0973-1296.153078.CrossRefPubMedPubMedCentral

Title: In vitro antibacterial activity of Loxostylis alata extracts and isolated compounds against Salmonella species
Authors: Dorcas A. Gado
Muna Ali Abdalla
Aroke S. Ahmed
Balungile Madikizela
Sanah M. Nkadimeng
Marthie M. Ehlers
Lyndy J. McGaw
Publication date: 01-12-2021
Publisher: BioMed Central
Published in: BMC Complementary Medicine and Therapies / Issue 1/2021
Electronic ISSN: 2662-7671
DOI: https://doi.org/10.1186/s12906-021-03292-4

Keynote webinar | Spotlight on medication adherence

Springer Medicine

In vitro antibacterial activity of Loxostylis alata extracts and isolated compounds against Salmonella species

Abstract

Background

Methods

Results

Conclusions

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Background

Methods

Results

Conclusions

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