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BMC Complementary Medicine and Therapies
Published in: BMC Complementary Medicine and Therapies 1/2024

Open Access 01-12-2024 | Research

In vitro antibacterial activity of fruit pulp extracts of Tamarindus indica against Staphylococcus aureus and Klebsiella pneumoniae

Authors: Gatluak Goanar, Geremew Tafesse, Workineh Mengesha Fereja

Published in: BMC Complementary Medicine and Therapies | Issue 1/2024

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Abstract

Background

Infectious diseases are increasingly recognized as public health concern worldwide as the rising incidence in multidrug resistance bacteria. This consequently enforces the need to find a new antimicrobial agent where plants have a potential source. This study investigated the antibacterial activity of fruit pulp extract of the Tamarindus indica against Staphylococcus aureus (S. aureus) and Klebsiella pneumoniae (K. pneumoniae).

Methods and materials

Maceration technique was employed for subsequent extraction of the sample using acetone and ethanol. Antibacterial activity of the plant extract was investigated based on minimum inhibitory concentration (MIC) against Gram-negative strain (K. pneumoniae (ATCC 700603)) and Gram-positive strain (S. aureus (ATCC 25923)) using agar disc-diffusion technique.

Results

It was found that both acetone and ethanol extracts showed significant antibacterial activities, against both S. aureus and K. pneumoniae as compared to the negative control (P = 0.00), but no significantly different from the drug (P > 0.05). However, K. pneumoniae showed more sensitivity to the extracts than S. aureus with MIC value of 18.75 mg/mL and 9.38 mg/mL for both acetone and ethanol extracts against S. aureus and K. pneumoniae respectively.

Conclusion

This study suggested that the fruit pulp have antibacterial properties, which might validate their traditional uses.
Literature
1.
Chassagne F, Samarakoon T, Porras G, Lyles JT, Dettweiler M, Marquez L, et al. A systematic review of plants with antibacterial activities: a taxonomic and phylogenetic perspective. Front Pharmacol. 2021;11:2069.CrossRef
2.
Olusola-Makinde OO, Bayode MT. Comparative antimicrobial study of Vernonia amygdalina Del. And Lawsonia inermis L. against microorganisms from aqueous milieu. Eur J Biol Res. 2021;11(3):283–93.
3.
Elisha IL, Botha FS, McGaw LJ, Eloff JN. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complement Altern Med. 2017;17(1):1–10.CrossRef
4.
5.
Nureye D, Salahaddin M, Woldeselassie W. One health approach and antimicrobial resistance: from global to Ethiopian context. EC Pharmacol Toxicol. 2020;8:59–75.
6.
Stokes JM, Yang K, Swanson K, Jin W, Cubillos-Ruiz A, Donghia NM, et al. Bloom-Ackermann Z: a deep learning approach to antibiotic discovery. Cell. 2020;180(4):688–702.e613.PubMedPubMedCentralCrossRef
7.
Van Goethem N, Struelens MJ, De Keersmaecker SC, Roosens NH, Robert A, Quoilin S, et al. Perceived utility and feasibility of pathogen genomics for public health practice: a survey among public health professionals working in the field of infectious diseases, Belgium, 2019. BMC Public Health. 2020;20(1):1–18.
8.
Bitew H, Gebregergs H, Tuem KB, Yeshak MY. Ethiopian medicinal plants traditionally used for wound treatment: a systematic review. Ethiop J Health Dev. 2019;33(2)
9.
Njeru SN, Matasyoh J, Mwaniki CG, Mwendia CM, Kobia K. A review of some phytochemicals commonly found in medicinal plants. Int J Med Plant. 2013;105:135–40.
10.
Pagare S, Bhatia M, Tripathi N, Pagare S, Bansal Y. Secondary metabolites of plants and their role: overview. Current Trends in Biotechnology and Pharmacy. 2015;9(3):293–304.
11.
12.
Salmerón-Manzano E, Garrido-Cardenas JA, Manzano-Agugliaro F. Worldwide research trends on medicinal plants. Int J Environ Res Public Health. 2020;17(10):3376.PubMedPubMedCentralCrossRef
13.
Vaou N, Stavropoulou E, Voidarou C, Tsigalou C, Bezirtzoglou E. Towards advances in medicinal plant antimicrobial activity: a review study on challenges and future perspectives. Microorganisms. 2021;9(10):2041.PubMedPubMedCentralCrossRef
14.
Tafroji W, Margyaningsih NI, Khoeri MM, Paramaiswari WT, Winarti Y, Salsabila K, et al. Antibacterial activity of medicinal plants in Indonesia on Streptococcus pneumoniae. PloS One. 2022;17(9):e0274174.PubMedPubMedCentralCrossRef
15.
Kassaye KD, Amberbir A, Getachew B, Mussema Y. A historical overview of traditional medicine practices and policy in Ethiopia. Ethiop J Health Dev. 2006;20(2):127–34.
16.
Gonfa N, Tulu D, Hundera K, Raga D. Ethnobotanical study of medicinal plants, its utilization, and conservation by indigenous people of Gera district, Ethiopia. Cogent Food Agric. 2020;6(1):1852716.CrossRef
17.
Belayneh A, Asfaw Z, Demissew S, Bussa NF. Medicinal plants potential and use by pastoral and agro-pastoral communities in Erer Valley of Babile Wereda, eastern Ethiopia. J Ethnobiol Ethnomed. 2012;8(1):1–11.CrossRef
18.
Fenetahun Y, Eshetu G. A review on ethnobotanical studies of medicinal plants use by agro-pastoral communities in Ethiopia. J Med Plants. 2017;5(1):33–44.
19.
Assefa B, Megersa M, Jima TT. Ethnobotanical study of medicinal plants used to treat human diseases in Gura Damole District, bale zone, Southeast Ethiopia. Asian J Ethnobiol. 2021;4(1)
20.
Tariq M, Chaudhary SS, Rahman K, Hamiduddin ZR, Imtiyaz S. Tamarindus indica: an overview. J Biol Scientific Opinion. 2013;1(2):128–31.CrossRef
21.
Muzaffar K, Kumar P. Tamarind-a mini review. MOJ Food Process Technol. 2017;5(3):296–7.CrossRef
22.
Chimsah F, Nyarko G, Abubakari A. A review of explored uses and study of nutritional potential of tamarind (Tamarindus indica L.) in Northern Ghana. 2020.
23.
Kapur MA, John SA. Antimicrobial activity of ethanolic bark extract of Tamarindus indica against some pathogenic microorganisms. Int J Curr Microbiol App Sci. 2014;3:589–93.
24.
Das MP, Banerjee A. Extraction of tamarind pulp and its antibacterial activity; 2011.
25.
Menezes APP, Trevisan SCC, Barbalho SM, Guiguer EL. Tamarindus indica L. A plant with multiple medicinal purposes. J Pharmacogn Phytochem. 2016;5(3):50–4.
26.
27.
Ismael J, Dessalegn E, Fereja WM. In vitro antioxidant and antibacterial activity of leaf extracts of Measa lanceolata. Int J Food Prop. 2021;24(1):702–12.CrossRef
28.
Ren G, Li L, Hu H, Li Y, Liu C, Wei S. Influence of the environmental factors on the accumulation of the bioactive ingredients in Chinese rhubarb products. PloS One. 2016;11(5):e0154649.PubMedPubMedCentralCrossRef
29.
Jeyaseelan EC, Jenothiny S, Pathmanathan M, Jeyadevan J. Antibacterial activity of sequentially extracted organic solvent extracts of fruits, flowers and leaves of Lawsonia inermis L. from Jaffna. Asian Pac J Trop Biomed. 2012;2(10):798–802.PubMedPubMedCentralCrossRef
30.
Balouiri M, Sadiki M, Ibnsouda SK. Methods for in vitro evaluating antimicrobial activity: a review. J Pharm Anal. 2016;6(2):71–9.PubMedCrossRef
31.
Tafesse G, Mekonnen Y, Makonnen E, Majinda RR, Bojase-Moleta G, Yeboah SO. Antibacterial activity of crude extracts and pure compounds isolated from Vernonia galamensis leaves. Afr J Pharm Pharmacol. 2018;12(11):136–41.CrossRef
32.
Bahiru M, Tafesse G, Chauhan NM, Assefa E. Antimicrobial activity of crude extract from Millettia ferruginea leaves and barks against selected bacterial pathogens and Candida albicans. J Microbiol Antimicrob. 2020;12(2):81–7.
33.
Onivogui G, Letsididi R, Diaby M, Wang L, Song Y. Influence of extraction solvents on antioxidant and antimicrobial activities of the pulp and seed of Anisophyllea laurina R. Br. ex Sabine fruits. Asian Pac J Trop Biomed. 2016;6(1):20–5.CrossRef
34.
Sharma A, Chandraker S, Patel V, Ramteke P. Antibacterial activity of medicinal plants against pathogens causing complicated urinary tract infections. Indian J Pharm Sci. 2009;71(2):136.PubMedPubMedCentralCrossRef
35.
Vashist H, Jindal A. Antimicrobial activities of medicinal plants–review. Int J Res Pharmaceut Biomed Sci. 2012;3(1):222–30.
36.
37.
Desta B. Ethiopian traditional herbal drugs. Part II: antimicrobial activity of 63 medicinal plants. J Ethnopharmacol. 1993;39(2):129–39.PubMedCrossRef
38.
Dubale S, Kebebe D, Zeynudin A, Abdissa N, Suleman S. Phytochemical screening and antimicrobial activity evaluation of selected medicinal plants in Ethiopia. J Exp Pharmacol. 2023;15:51–62.PubMedPubMedCentralCrossRef
39.
Nigussie D, Davey G, Tufa TB, Brewster M, Legesse BA, Fekadu A, et al. Antibacterial and antifungal activities of Ethiopian medicinal plants: a systematic review. Front Pharmacol. 2021;12:633921.PubMedPubMedCentralCrossRef
40.
Attallah NG, Al-Fakhrany OM, Elekhnawy E, Hussein IA, Shaldam MA, Altwaijry N, et al. Anti-biofilm and antibacterial activities of Cycas media R. Br secondary metabolites: In silico, in vitro, and in vivo approaches. Antibiotics. 2022;11(8):993.PubMedPubMedCentralCrossRef
41.
Adhikari P, Joshi K, Singh M, Pandey A. Influence of altitude on secondary metabolites, antioxidants, and antimicrobial activities of Himalayan yew (Taxus wallichiana). Plant Biosyst-An Int J Deal with all Asp of Plant Bio. 2022;156(1):187–95.
42.
Wallace RJ. Antimicrobial properties of plant secondary metabolites. Proc Nutr Soc. 2004;63(4):621–9.PubMedCrossRef
43.
Abdallah M, Muhammad A. Antibacterial activity of leaves and fruits extract of Tamarindus indica against clinical isolates of Escherichia coli and Shigella at Potiskum Yobe state, Nigeria. J Anal Pharm Res. 2018;7(5):606–9.
44.
Dorcas K, Palakodeti P, Yadav H, Yerra V, Majjiga V. Antibacterial properties of tamarindus; 2020.
45.
Fagbemi KO, Aina DA, Adeoye-Isijola MO, Naidoo KK, Coopoosamy RM, Olajuyigbe OO. Bioactive compounds, antibacterial and antioxidant activities of methanol extract of Tamarindus indica Linn. Sci Rep. 2022;12(1):9432.ADSPubMedPubMedCentralCrossRef
46.
Kuru P. Tamarindus indica and its health related effects. Asian Pac J Trop Biomed. 2014;4(9):676–81.CrossRef
47.
Gomathinayagam S, Tewari B, Rekha G. Heavy metal and phytochemical studies of crude leaf extract of tamarind (Tamarindus indica). Adv Life Sci. 2017;7:1–4.
Metadata
Title
In vitro antibacterial activity of fruit pulp extracts of Tamarindus indica against Staphylococcus aureus and Klebsiella pneumoniae
Authors
Gatluak Goanar
Geremew Tafesse
Workineh Mengesha Fereja
Publication date
01-12-2024
Publisher
BioMed Central
Published in
BMC Complementary Medicine and Therapies / Issue 1/2024
Electronic ISSN: 2662-7671
DOI
https://doi.org/10.1186/s12906-024-04404-6

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