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World Journal of Urology

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01-02-2009 | Original Article

Immunoscreening of urinary bladder cancer cDNA library and identification of potential tumor antigen

Authors: Ling Chen, Wei Chen, Le Zhao, Hai-Zhen Yu, Xu Li

Published in: World Journal of Urology | Issue 1/2009

Abstract

Objective

To identify potential tumor antigen by immunoscreening the urinary bladder cancer cDNA library with monoclonal antibodies.

Methods

Monoclonal antibodies were prepared. A cDNA expression library was constructed from bladder cancer cell line BLZ211. Immunogenic proteins were identified by immunoscreening the cDNA library with ten monoclonal antibodies.

Results

The cDNA library of BLZ211 cells was established using λZAP as vector. The titer of unamplified cDNA library was 1.39×10⁶ pfu/ml with a recombinant rate of 97.72%, and titer of amplified one was 8.4×10⁹ pfu/ml. After immunoscreening, ten positive clones representing ten different antigens were identified, which include two proteins with unknown function; coactosin-like 1, eukaryotic translation elongation factor, HNRPA1, histidine triad nucleotide binding protein, KRT7, LCN2, TSTA3, zinc finger protein, C11orf48 and HSPC148.

Conclusion

The cDNA library was of high quality and can be used in further study. By immunoscreening the bladder cancer cDNA library with ten monoclonal antibodies, we identified ten immunogenic proteins that otherwise would not have been identified as potential diagnostic marker and vaccinogens of bladder cancer using the gene discovery effort.

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Title: Immunoscreening of urinary bladder cancer cDNA library and identification of potential tumor antigen
Authors: Ling Chen
Wei Chen
Le Zhao
Hai-Zhen Yu
Xu Li
Publication date: 01-02-2009
Publisher: Springer-Verlag
Published in: World Journal of Urology / Issue 1/2009
Print ISSN: 0724-4983
Electronic ISSN: 1433-8726
DOI: https://doi.org/10.1007/s00345-008-0326-4

At a glance: The STEP trials

Springer Medicine

Immunoscreening of urinary bladder cancer cDNA library and identification of potential tumor antigen

Abstract

Objective

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Objective

Methods

Results

Conclusion

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