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Tumor Biology
Published in: Tumor Biology 6/2016

01-06-2016 | Original Article

Immunomodulatory and cellular antioxidant activities of pure compounds from Teucrium ramosissimum Desf.

Authors: Nouha Nasr-Bouzaiene, Aicha Sassi, Ahmed Bedoui, Mounira Krifa, Leila Chekir-Ghedira, Kamel Ghedira

Published in: Tumor Biology | Issue 6/2016

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Abstract

Evaluation of the immunomodulatory activity of plant compounds is an interesting and growing area of research. Teucrium ramosissimum Desf. is a native and endemic medicinal plant from the South of Tunisia traditionally used for the treatment of many diseases. The anti-inflammatory activity of apigenin-7-glucoside, genkwanin, and naringenin isolated from T. ramosissimum were assayed. The phagocytic activities of macrophage and lymphocyte proliferation were investigated in the absence and presence of mitogens (lipopolysaccharide [LPS] or lectin). Depending on the concentrations, the compounds affect macrophage functions by modulating their lysosomal enzyme activity and nitric oxide (NO) release. The tested compounds enhance significantly splenocyte proliferation, either with or without mitogen stimulation. In studies to assess any potential effects of apigenin-7-glucoside, genkwanin, and naringenin on innate immunity, the results showed that these compounds significantly enhanced the killing activity of natural killer (NK) cells and cytotoxic activity of the T lymphocyte (CTL) isolated from splenocytes. These results suggest that T. ramosissimum compounds such as apigenin-7-glucoside, genkwanin, and naringenin may be potentially useful for modulating immune cell functions in physiological and pathological conditions.
Literature
1.
Bello R, Barrachina M, Martínez-Cuesta M, Esplugues J, Yufera EP. Evaluation of the acute toxicity, analgesic and CNS activities of different species of Teucrium genus. Phytother Res. 1995;9:277–80.CrossRef
2.
Abdollahi M, Karimpour H, Monsef-Esfehani HR. Antinociceptive effects of Teucrium polium L total extract and essential oil in mouse writhing test. Pharmacol Res: Off J Italian Pharmacol Soc. 2003;48:31–5.CrossRef
3.
Menichini F, Conforti F, Rigano D, Formisano C, Piozzi F, Senatore F. Phytochemical composition, anti-inflammatory and antitumour activities of four Teucrium essential oils from Greece. Food Chem. 2009;115:679–86.CrossRef
4.
Pottier-Alapetite G. Flore de la Tunisie, Angiospermes-Dicotyledones Gamopetales. Première partie. MES & MA, 1979;764--777.
5.
Limem I, Harizi H, Ghedira K, Chekir-Ghedira L. Leaf extracts from Phlomis crinita Cav. subs. mauritanica Munby affect immune cell functions in vitro. Immunopharmacol Immunotoxicol. 2011;33:309–14.CrossRefPubMed
6.
Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods. 1983;65:55–63.CrossRefPubMed
7.
Manosroi A, Saraphanchotiwitthaya A, Manosroi J. Immunomodulatory activities of Clausena excavata Burm. f. wood extracts. J Ethnopharmacol. 2003;89:155–60.CrossRefPubMed
8.
Sarangi I, Ghosh D, Bhutia SK, Mallick SK, Maiti TK. Anti-tumor and immunomodulating effects of Pleurotus ostreatus mycelia-derived proteoglycans. Int Immunopharmacol. 2006;6:1287–97.CrossRefPubMed
9.
Manosroi A, Saraphanchotiwitthaya A, Manosroi J. In vitro immunomodulatory effect of Pouteria cambodiana (Pierre ex Dubard) Baehni extract. J Ethnopharmacol. 2005;101:90–4.CrossRefPubMed
10.
Green LC, Wagner DA, Glogowski J, Skipper PL, Wishnok JS, Tannenbaum SR. Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem. 1982;126:131–8.CrossRefPubMed
11.
Wolfe KL, Liu RH. Cellular antioxidant activity (CAA) assay for assessing antioxidants, foods, and dietary supplements. J Agric Food Chem. 2007;55:8896–907.CrossRefPubMed
12.
Wang D, Ma Y, Wang J, Liu X, Fang M. Natural killer cells in innate defense against infective pathogens. J Clin Cell Immunol S. 2013;13:2.
13.
Kagi D, Ledermann B, Burki K, Hengartner H, Zinkernagel RM. Cd8+ T cell-mediated protection against an intracellular bacterium by perforin-dependent cytotoxicity. Eur J Immunol. 1994;24:3068–72.CrossRefPubMed
14.
Dubick MA. Historical perspectives on the use of herbal preparations to promote health. J Nutr. 1986;116:1348–54.PubMed
15.
Tapsell LC, Hemphill I, Cobiac L, Patch CS, Sullivan DR, Fenech M, et al. Health benefits of herbs and spices: the past, the present, the future. Med J Aust. 2006;185:S4–24.PubMed
16.
Harizi H, Chaabane F, Ghedira K, Chekir-Ghedira L. Inhibition of proinflammatory macrophage responses and lymphocyte proliferation in vitro by ethyl acetate leaf extract from Daphne gnidium. Cell Immunol. 2011;267:94–101.CrossRefPubMed
17.
Krifa M, Bouhlel I, Ghedira-Chekir L, Ghedira K. Immunomodulatory and cellular anti-oxidant activities of an aqueous extract of Limoniastrum guyonianum gall. J Ethnopharmacol. 2013;146:243–9.CrossRefPubMed
18.
Krifa M, Mustapha N, Ghedira Z, Ghedira K, Pizzi A, Chekir-Ghedira L. Limoniastrum guyonianum methanol extract induces immunomodulatory and anti-inflammatory effects by activating cellular anti-oxidant activity. Drug Chem Toxicol. 2015;38:84–91.CrossRefPubMed
19.
Ben Sghaier M, Krifa M, Mensi R, Bhouri W, Ghedira K, Chekir-Ghedira L. In vitro and in vivo immunomodulatory and anti-ulcerogenic activities of Teucrium ramosissimum extracts. J Immunotoxicol. 2011;8:288–97.CrossRefPubMed
20.
Nakamura A, Nagai K, Suzuki S, Ando K, Tamura G. A novel method of screening for immunomodulating substances, establishment of an assay system and its application to culture broths of microorganisms. J Antibiot. 1986;39:1148–54.CrossRefPubMed
21.
Ljunggren HG, Karre K. In search of the ‘missing self’: MHC molecules and NK cell recognition. Immunol Today. 1990;11:237–44.CrossRefPubMed
22.
Ismail N, Abdullah H, Seidel V, Rotondo D. Human natural killer (NK) cell activation by luteolin from Brucea javanica leaves. J Cancer Res Exp Oncol. 2012.
23.
Krifa M, El Mekdad H, Bentouati N, Pizzi A, Ghedira K, Hammami M, et al. Immunomodulatory and anticancer effects of Pituranthos tortuosus essential oil. Tumour Biol: J Int Soc Oncodevelopmental Biol Med. 2015.
24.
Lindqvist C, Bobrowska-Hagerstrand M, Mrowczynska L, Engblom C, Hagerstrand H. Potentiation of natural killer cell activity with myricetin. Anticancer Res. 2014;34:3975–9.PubMed
25.
Whiteside TL, Herberman RB. The role of natural killer cells in immune surveillance of cancer. Curr Opin Immunol. 1995;7:704–10.CrossRefPubMed
26.
Sheeja K, Kuttan G. Activation of cytotoxic T lymphocyte responses and attenuation of tumor growth in vivo by Andrographis paniculata extract and andrographolide. Immunopharmacol Immunotoxicol. 2007;29:81–93.CrossRefPubMed
27.
Guo TL, Chi RP, Hernandez DM, Auttachoat W, Zheng JF. Decreased 7,12-dimethylbenz[a]anthracene-induced carcinogenesis coincides with the induction of antitumor immunities in adult female B6C3F1 mice pretreated with genistein. Carcinogenesis. 2007;28:2560–6.CrossRefPubMedPubMedCentral
28.
Shi X, Ruan D, Wang Y, Ma L, Li M. [Anti-tumor activity of safflower polysaccharide (SPS) and effect on cytotoxicity of CTL cells, NK cells of T739 lung cancer in mice]. Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China J Chin Mater Med. 2010;35:215–8.
29.
Trapani JA, Smyth MJ. Functional significance of the perforin/granzyme cell death pathway. Nat Rev Immunol. 2002;2:735–47.CrossRefPubMed
30.
Siveen KS, Kuttan G. Effect of Aerva lanata on cell-mediated immune responses and cytotoxic T-lymphocyte generation in normal and tumor-bearing mice. J Immunotoxicol. 2012;9:25–33.CrossRefPubMed
31.
Herberman RB. Assessment of cellular immune response to cancer of the breast. Ann Clin Lab Sci. 1979;9:467–73.PubMed
32.
33.
Schepetkin IA, Quinn MT. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential. Int Immunopharmacol. 2006;6:317–33.CrossRefPubMed
34.
Jakhar R, Paul S, Chauhan AK, Kang SC. Morin hydrate augments phagocytosis mechanism and inhibits LPS induced autophagic signaling in murine macrophage. Int Immunopharmacol. 2014;22:356–65.CrossRefPubMed
35.
MacMicking J, Xie QW, Nathan C. Nitric oxide and macrophage function. Annu Rev Immunol. 1997;15:323–50.CrossRefPubMed
36.
Tamir S, Tannenbaum SR. The role of nitric oxide (NO.) in the carcinogenic process. Biochim Biophys Acta. 1996;1288:F31–36.PubMed
37.
Sagnia B, Fedeli D, Casetti R, Montesano C, Falcioni G, Colizzi V. Antioxidant and anti-inflammatory activities of extracts from Cassia alata, Eleusine indica, Eremomastax speciosa, Carica papaya and Polyscias fulva medicinal plants collected in Cameroon. PLoS One. 2014;9, e103999.CrossRefPubMedPubMedCentral
38.
Estany S, Palacio JR, Barnadas R, Sabes M, Iborra A, Martinez P. Antioxidant activity of N-acetylcysteine, flavonoids and alpha-tocopherol on endometrial cells in culture. J Reprod Immunol. 2007;75:1–10.CrossRefPubMed
Metadata
Title
Immunomodulatory and cellular antioxidant activities of pure compounds from Teucrium ramosissimum Desf.
Authors
Nouha Nasr-Bouzaiene
Aicha Sassi
Ahmed Bedoui
Mounira Krifa
Leila Chekir-Ghedira
Kamel Ghedira
Publication date
01-06-2016
Publisher
Springer Netherlands
Published in
Tumor Biology / Issue 6/2016
Print ISSN: 1010-4283
Electronic ISSN: 1423-0380
DOI
https://doi.org/10.1007/s13277-015-4635-0

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