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Open Access 01-12-2007 | Short report

Immunization with HIV protease peptides linked to syngeneic erythrocytes

Authors: Andreas Boberg, Sabrina Dominici, Andreas Brave, Kristian Hallermalm, Jorma Hinkula, Mauro Magnani, Britta Wahren

Published in: Infectious Agents and Cancer | Issue 1/2007

Abstract

New potent vaccine adjuvants are desirable for increasing the efficacy of novel vaccine modalities such as DNA and peptides. We therefore tested if syngeneic erythrocytes could serve as delivery vectors for selected HIV peptides and compared the potency of these constructs to immunization with peptides in phosphate buffered saline or in incomplete Freunds adjuvant.

Immunization of mice with peptides in a low dose (5 ng) coupled to erythrocytes induced a weak immune response in mice. These peptides alone (5 μg) gave no immune responses, while formulating the peptides (50 μg) in IFA induced strong homologous immunity as well as prominent cross reactivity to a related mutant epitope. Thus, vaccine delivery using syngeneic erythrocytes, although attractive for clinical use, might be of limited value due to the low amount of antigen that can be loaded per erythrocyte.

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Title: Immunization with HIV protease peptides linked to syngeneic erythrocytes
Authors: Andreas Boberg
Sabrina Dominici
Andreas Brave
Kristian Hallermalm
Jorma Hinkula
Mauro Magnani
Britta Wahren
Publication date: 01-12-2007
Publisher: BioMed Central
Published in: Infectious Agents and Cancer / Issue 1/2007
Electronic ISSN: 1750-9378
DOI: https://doi.org/10.1186/1750-9378-2-9

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