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01-05-2014 | Original Article

Imbalance of Ca²⁺ and K⁺ fluxes in C6 glioma cells after PDT measured with scanning ion-selective electrode technique

Authors: Sheng-Li Hu, Peng Du, Rong Hu, Fei Li, Hua Feng

Published in: Lasers in Medical Science | Issue 3/2014

Abstract

Photodynamic therapy (PDT) possesses the capacity to lead to death of C6 glioma in vitro and in vivo. The purpose of this study was to investigate whether Ca²⁺ and K⁺ homeostasis of C6 glioma cells were affected by PDT. C6 glioma cells were randomly divided into five groups: control group, Hematoporphyrin derivative (HpD) group (10 mg/l, without irradiation), PDT group (HpD 10 mg/l + irradiation), PDT&6-Cyano-7-nitroquinoxaline-2,3-dione (CNQX) group (HpD 10 mg/l + CNQX 50 mol/l + irradiation), and HpD&CNQX group (HpD 10 mg/l + CNQX 50 mol/l, without irradiation). Glioma cells in PDT and PDT&CNQX group were subjected to PDT. Cells in PDT&CNQX group were administered α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) glutamate receptor antagonist CNQX prior to PDT on C6 glioma cells. The changes of Ca²⁺ and K⁺ fluxes were studied by using a non-invasive scanning ion-selective electrode technique (SIET). Morphology of C6 cells was observed with optical microscopy. PDT induced Ca²⁺ influx and K⁺ efflux significantly, which resulted in death of C6 cells. When AMPA glutamate receptor antagonist CNQX was applied, Ca²⁺ influx and K⁺ efflux were partly blocked up and viability of C6 cells increased. These results indicate that Ca²⁺ influx and K⁺ efflux may correlate with the treatment effects of PDT on C6 glioma cells.

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Title: Imbalance of Ca2+ and K+ fluxes in C6 glioma cells after PDT measured with scanning ion-selective electrode technique
Authors: Sheng-Li Hu
Peng Du
Rong Hu
Fei Li
Hua Feng
Publication date: 01-05-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1518-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Imbalance of Ca²⁺ and K⁺ fluxes in C6 glioma cells after PDT measured with scanning ion-selective electrode technique

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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