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European Journal of Nuclear Medicine and Molecular Imaging
Published in: European Journal of Nuclear Medicine and Molecular Imaging 7/2016

01-07-2016 | Original Article

Imaging of brain TSPO expression in a mouse model of amyotrophic lateral sclerosis with 18F-DPA-714 and micro-PET/CT

Authors: S. Gargiulo, S. Anzilotti, A. R. D. Coda, M. Gramanzini, A. Greco, M. Panico, A. Vinciguerra, A. Zannetti, C. Vicidomini, F. Dollé, G. Pignataro, M. Quarantelli, L. Annunziato, A. Brunetti, M. Salvatore, S. Pappatà

Published in: European Journal of Nuclear Medicine and Molecular Imaging | Issue 7/2016

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Abstract

Purpose

To evaluate the feasibility and sensitivity of 18F-DPA-714 for the study of microglial activation in the brain and spinal cord of transgenic SOD1G93A mice using high-resolution PET/CT and to evaluate the Iba1 and TSPO expression with immunohistochemistry.

Methods

Nine symptomatic SOD1G93A mice (aged 117 ± 12.7 days, clinical score range 1 – 4) and five WT SOD1 control mice (aged 108 ± 28.5 days) underwent 18F-DPA-714 PET/CT. SUV ratios were calculated by normalizing the cerebellar (rCRB), brainstem (rBS), motor cortex (rMCX) and cervical spinal cord (rCSC) activities to that of the frontal association cortex. Two WT SOD1 and six symptomatic SOD1G93A mice were studied by immunohistochemistry.

Results

In the symptomatic SOD1G93A mice, rCRB, rBS and rCSC were increased as compared to the values in WT SOD1 mice, with a statistically significantly difference in rBS (2.340 ± 0.784 vs 1.576 ± 0.287, p = 0.014). Immunofluorescence studies showed that TSPO expression was increased in the trigeminal, facial, ambiguus and hypoglossal nuclei, as well as in the spinal cord, of symptomatic SOD1G93A mice and was colocalized with increased Iba1 staining.

Conclusion

Increased 18F-DPA-714 uptake can be detected with high-resolution PET/CT in the brainstem of transgenic SOD1G93A mice, a region known to be a site of degeneration and increased microglial activation in amyotrophic lateral sclerosis, in agreement with increased TSPO expression in the brainstem nuclei shown by immunostaining. Therefore, 18F-DPA-714 PET/CT might be a suitable tool to evaluate microglial activation in the SOD1G93A mouse model.
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Metadata
Title
Imaging of brain TSPO expression in a mouse model of amyotrophic lateral sclerosis with 18F-DPA-714 and micro-PET/CT
Authors
S. Gargiulo
S. Anzilotti
A. R. D. Coda
M. Gramanzini
A. Greco
M. Panico
A. Vinciguerra
A. Zannetti
C. Vicidomini
F. Dollé
G. Pignataro
M. Quarantelli
L. Annunziato
A. Brunetti
M. Salvatore
S. Pappatà
Publication date
01-07-2016
Publisher
Springer Berlin Heidelberg
Published in
European Journal of Nuclear Medicine and Molecular Imaging / Issue 7/2016
Print ISSN: 1619-7070
Electronic ISSN: 1619-7089
DOI
https://doi.org/10.1007/s00259-016-3311-y

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