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01-12-2009 | Original Article

Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats

Authors: Marleen T. J. van Ampting, Wendy Rodenburg, Carolien Vink, Evelien Kramer, Arjan J. Schonewille, Jaap Keijer, Roelof van der Meer, Ingeborg M. J. Bovee-Oudenhoven

Published in: Digestive Diseases and Sciences | Issue 12/2009

Abstract

Background Microbial infections induce ileal pancreatitis-associated protein/regenerating gene III (PAP/RegIII) mRNA expression. Despite increasing interest, little is known about the PAP/RegIII protein. Therefore, ileal mucosal PAP/RegIII protein expression, localization, and fecal excretion were studied in rats upon Salmonella infection. Results Salmonella infection increased ileal mucosal PAP/RegIII protein levels in enterocytes located at the crypt-villus junction. Increased colonization and translocation of Salmonella was associated with higher ileal mucosal PAP/RegIII levels and secretion of this protein in feces. Conclusions PAP/RegIII protein is increased in enterocytes of the ileal mucosa during Salmonella infection and is associated with infection severity. PAP/RegIII is excreted in feces and might be used as a new and non-invasive infection marker.

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Title: Ileal Mucosal and Fecal Pancreatitis Associated Protein Levels Reflect Severity of Salmonella Infection in Rats
Authors: Marleen T. J. van Ampting
Wendy Rodenburg
Carolien Vink
Evelien Kramer
Arjan J. Schonewille
Jaap Keijer
Roelof van der Meer
Ingeborg M. J. Bovee-Oudenhoven
Publication date: 01-12-2009
Publisher: Springer US
Published in: Digestive Diseases and Sciences / Issue 12/2009
Print ISSN: 0163-2116
Electronic ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-008-0685-0

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