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01-11-2010 | Laboratory Investigation - Human/Animal Tissue

IL-6 promotion of glioblastoma cell invasion and angiogenesis in U251 and T98G cell lines

Authors: Qinglin Liu, Gang Li, Ronghui Li, Jie shen, Qiaowei He, Lin Deng, Cai Zhang, Jian Zhang

Published in: Journal of Neuro-Oncology | Issue 2/2010

Abstract

Interleukin-6 (IL-6) is a growth and survival factor in human glioblastoma cells and plays an important role in malignant progression. However, its role in glioblastoma invasion is still unknown. This study shows how IL-6 promotes cell invasion and migration in U251 and T98G glioblastoma cell lines. The underlying mechanism includes both protease-dependent and -independent manners. Stimulation with IL-6 increased MMP9 expression in the two cell lines but had no influence on MMP2 expression. Fascin-1 is a cell skeleton binding protein and plays a key role in cell migration and invasion. Its binding style directly influences cell morphology and tendency to become deformed. After IL-6 exposure, fascin-1 expression increased in an IL-6 dose-dependent manner. Immunofluorescence also revealed that the binding style of fascin-1 had changed after IL-6 exposure, resulting in a more invasive phenotype of the cells. Three most commonly emphasized invasion-associated signaling pathways, including JAK-STAT3, p42/44 MAPK, and PI3K/AKT, were verified to further illustrate its underlying mechanism. Only phosphorylation of STAT3 at ser 727 site paralleled the IL-6 stimulation, and JSI-124, a specific JAK-STAT3 pathway blocker, deterred the invasion and migration promotive effect of IL-6, indicating that the JAK/STAT3 pathway mediates signal transduction. Furthermore, IL-6 also acts in a paracrine fashion to promote vascular endothelial cell migration, thus facilitating tumor angiogenesis and invasion. These results suggest that IL-6 promotes glioblastoma cell invasion and angiogenesis and may be a potential anti-invasion target.

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Title: IL-6 promotion of glioblastoma cell invasion and angiogenesis in U251 and T98G cell lines
Authors: Qinglin Liu
Gang Li
Ronghui Li
Jie shen
Qiaowei He
Lin Deng
Cai Zhang
Jian Zhang
Publication date: 01-11-2010
Publisher: Springer US
Published in: Journal of Neuro-Oncology / Issue 2/2010
Print ISSN: 0167-594X
Electronic ISSN: 1573-7373
DOI: https://doi.org/10.1007/s11060-010-0158-0

At a glance: The STEP trials

Springer Medicine

IL-6 promotion of glioblastoma cell invasion and angiogenesis in U251 and T98G cell lines

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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