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BMC Pediatrics
Published in: BMC Pediatrics 1/2021

Open Access 01-12-2021 | IgA Vasculitis | Research article

Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura

Authors: Ning Zhang, Ge Tian, Yuanyuan Sun, Jing Pan, Wei Xu, Zhe Li

Published in: BMC Pediatrics | Issue 1/2021

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Abstract

Aim

IgA-producing B cells have been found to be associated with children diagnosed with Henoch-Schonlein purpura (HSP). The aim of the present study was to determine whether children with HSP possess altered B-cell subsets.

Methods

A total of 14 children diagnosed with HSP and age- and sex-matched healthy controls (HCs) were enrolled in our study. Peripheral blood mononuclear cells were isolated, and the percentage and absolute number of B-cell subsets and Follicular helper T (Tfh) cells were determined by flow cytometry. Finally, Spearman’s correlation coefficient was used to analyse the correlation between the percentage of Tfh cells and B-cell subsets.

Results

We found that compared to HCs, the frequency and absolute number of total B cells were significantly higher in children with HSP, but the percentages of plasma cells and naïve B cells were significantly lower. A significantly increased percentage and absolute number of memory nonswitched B cells were found in children with HSP compared with HCs. We observed that the expression of C-X-C chemokine receptor type 5 (CXCR5) on total CD4+ T cells and the percentage of CD4+CXCR5+ cells were significantly increased in patients with HSP. Moreover, significantly correlations between Tfh cells and various B-cell subsets were observed.

Conclusions

Our study showed a Tfh-cell-associated altered B cell compartment in children with HSP.
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Metadata
Title
Altered B cell compartment associated with Tfh cells in children with Henoch-Schonlein Purpura
Authors
Ning Zhang
Ge Tian
Yuanyuan Sun
Jing Pan
Wei Xu
Zhe Li
Publication date
01-12-2021
Publisher
BioMed Central
Published in
BMC Pediatrics / Issue 1/2021
Electronic ISSN: 1471-2431
DOI
https://doi.org/10.1186/s12887-021-02873-z

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