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Virchows Archiv
Published in: Virchows Archiv 2/2006

01-08-2006 | Original Article

Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours

Authors: Si Brask Sonne, Amy S. Herlihy, Christina E. Hoei-Hansen, John E. Nielsen, Kristian Almstrup, Niels E. Skakkebaek, Alexander Marks, Henrik Leffers, Ewa Rajpert-De Meyts

Published in: Virchows Archiv | Issue 2/2006

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Abstract

Testicular germ-cell tumours of young adults are derived from a pre-invasive intratubular lesion, carcinoma in situ (CIS). In a recent genome-wide gene expression screening using cDNA microarrays, we found PDPN over-expressed in CIS compared to normal adult testis. PDPN encodes podoplanin (Aggrus, human gp36, T1A-2), a transmembrane glycoprotein expressed in lymphatic endothelium and various solid tumours. To examine a potential role for PDPN in testicular neoplasms and during testicular development, we investigated its expression pattern during the development of human testis and in a series of testicular CIS, gonadoblastoma and overt germ-cell tumours. We established by RT-PCR and by immunohistochemistry with a gp36 antibody that PDPN mRNA and the protein product were expressed in testes with germ-cell neoplasms but not in the normal adult testis. We also found gp36 expression in early foetal gonocytes and immature Sertoli cells, similar to the expression pattern of M2A antigen, a previously identified marker for CIS and seminoma. This reinforced our previous proposal that M2A (D2-40) antigen was identical to gp36 (podoplanin, Aggrus, T1A-2). Our findings also suggest that podoplanin has a function in developing testis, most likely at the level of cell–cell interactions among pre-meiotic germ cells and immature Sertoli cells.
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Metadata
Title
Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours
Authors
Si Brask Sonne
Amy S. Herlihy
Christina E. Hoei-Hansen
John E. Nielsen
Kristian Almstrup
Niels E. Skakkebaek
Alexander Marks
Henrik Leffers
Ewa Rajpert-De Meyts
Publication date
01-08-2006
Publisher
Springer-Verlag
Published in
Virchows Archiv / Issue 2/2006
Print ISSN: 0945-6317
Electronic ISSN: 1432-2307
DOI
https://doi.org/10.1007/s00428-006-0223-4

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