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Chinese Medicine
Published in: Chinese Medicine 1/2015

Open Access 01-12-2015 | Research

Identification of Hippophae species (Shaji) through DNA barcodes

Authors: Yue Liu, Wei Sun, Chuan Liu, Yaqin Zhang, Yilong Chen, Ming Song, Gang Fan, Xia Liu, Li Xiang, Yi Zhang

Published in: Chinese Medicine | Issue 1/2015

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Abstract

Background

The morphological identification of different Hippophae species (Shaji) was difficult. This study aims to discriminate between medicinal and non-medicinal Hippophae species by DNA barcodes, the ITS2, psbA-trnH, and a combination of ITS2 and psbA-trnH (ITS2 + psbA-trnH).

Methods

DNA was extracted from the dried fruit samples. Primer pairs ITS2F/3R for ITS2 and psbAF/trnHR for psbA-trnH were used for PCR amplification. The purified PCR products were bidirectionally sequenced. Genetic distances were calculated according to the Kimura 2 parameter model and phylogenetic tree was constructed based on neighbor-joining (NJ) method, barcoding gap was also analyzed to assess identification efficiency.

Results

Amplification and sequencing efficiencies for both ITS2 and psbA-trnH were 100 %. Sequence data revealed that ITS2 + psbA-trnH was the most suitable candidate barcode at the species and subspecies level. The closely related Hippophae species were effectively differentiated in the NJ tree.

Conclusion

The combination of the two loci, ITS2 + psbA-trnH is applicable to the identification of medicinal and non-medicinal Hippophae species.
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Metadata
Title
Identification of Hippophae species (Shaji) through DNA barcodes
Authors
Yue Liu
Wei Sun
Chuan Liu
Yaqin Zhang
Yilong Chen
Ming Song
Gang Fan
Xia Liu
Li Xiang
Yi Zhang
Publication date
01-12-2015
Publisher
BioMed Central
Published in
Chinese Medicine / Issue 1/2015
Electronic ISSN: 1749-8546
DOI
https://doi.org/10.1186/s13020-015-0062-9

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