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European Journal of Clinical Microbiology & Infectious Diseases

Published in:

01-10-2012 | Article

Identification of group A streptococcal emm types commonly associated with invasive infections and antimicrobial resistance by the use of multiplex PCR and high-resolution melting analysis

Authors: P. Bidet, S. Liguori, C. Plainvert, S. Bonacorsi, C. Courroux, C. d’Humières, C. Poyart, A. Efstratiou, E. Bingen

Published in: European Journal of Clinical Microbiology & Infectious Diseases | Issue 10/2012

Abstract

M/emm typing, based either on serotyping of the M protein or on sequencing of the emm gene, is a major tool for epidemiological studies of group A streptococci (GAS). In order to simplify M/emm typing, we designed two multiplex polymerase chain reaction (PCR) formats capable of identifying the most frequent GAS M/emm types involved in invasive infections and antimicrobial resistance. A heptaplex PCR procedure was first developed in a conventional format coupled with gel electrophoresis to identify emm types 1, 3, 4, 6, 12, 28, and 89, based on the size of the amplification products. The other method, designed to identify the same seven emm types, together with emm11, was based on a real-time PCR format coupled with high-resolution melting (HRM) analysis, allowing the rapid typing of large strain collections.

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Title: Identification of group A streptococcal emm types commonly associated with invasive infections and antimicrobial resistance by the use of multiplex PCR and high-resolution melting analysis
Authors: P. Bidet
S. Liguori
C. Plainvert
S. Bonacorsi
C. Courroux
C. d’Humières
C. Poyart
A. Efstratiou
E. Bingen
Publication date: 01-10-2012
Publisher: Springer-Verlag
Published in: European Journal of Clinical Microbiology & Infectious Diseases / Issue 10/2012
Print ISSN: 0934-9723
Electronic ISSN: 1435-4373
DOI: https://doi.org/10.1007/s10096-012-1635-5

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