Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Breast Cancer Research
Published in: Breast Cancer Research 2/2003

Open Access 01-04-2003 | Research article

Id-1 is not expressed in the luminal epithelial cells of mammary glands

Authors: Norihisa Uehara, Yu-Chien Chou, Jose J Galvez, Paola de-Candia, Robert D Cardiff, Robert Benezra, Gopalan Shyamala

Published in: Breast Cancer Research | Issue 2/2003

Login to get access

Abstract

Background

The family of inhibitor of differentiation/DNA binding (Id) proteins is known to regulate development in several tissues. One member of this gene family, Id-1, has been implicated in mammary development and carcinogenesis. Mammary glands contain various cell types, among which the luminal epithelial cells are primarily targeted for proliferation, differentiation and carcinogenesis. Therefore, to assess the precise significance of Id-1 in mammary biology and carcinogenesis, we examined its cellular localization in vivo using immunohistochemistry.

Methods

Extracts of whole mammary glands from wild type and Id-1 null mutant mice, and tissue sections from paraffin-embedded mouse mammary glands from various developmental stages and normal human breast were subjected to immunoblot and immunohistochemical analyses, respectively. In both these procedures, an anti-Id-1 rabbit polyclonal antibody was used for detection of Id-1.

Results

In immunoblot analyses, using whole mammary gland extracts, Id-1 was detected. In immunohistochemical analyses, however, Id-1 was not detected in the luminal epithelial cells of mammary glands during any stage of development, but it was detected in vascular endothelial cells.

Conclusion

Id-1 is not expressed in the luminal epithelial cells of mammary glands.
Literature
1.
Israel MA, Hernandez MC, Florio M, Andres-Barquin PJ, Mantani A, Carter JH, Julin CM: Id gene expression as a key mediator of tumor cell biology. Cancer Res. 1999, 59: 1726s-1730s.PubMed
2.
Norton JD: ID helix–loop–helix proteins in cell growth, differentiation and tumorigenesis. J Cell Sci. 2000, 113: 3897-3905.PubMed
3.
Desprez PY, Hara E, Bissell MJ, Campisi J: Suppression of mammary epithelial cell differentiation by the helix–loop–helix protein Id-1. Mol Cell Biol. 1995, 15: 3398-3404.CrossRefPubMedPubMedCentral
4.
Yan W, Young AZ, Soares VC, Kelley R, Benezra R, Zhuang Y: High incidence of T-cell tumors in E2A-null mice and E2A/Id1 double-knockout mice. Mol Cell Biol. 1997, 17: 7317-7327.CrossRefPubMedPubMedCentral
5.
Sablitzky F, Moore A, Bromley M, Deed RW, Newton JS, Norton JD: Stage- and subcellular-specific expression of Id proteins in male germ and Sertoli cells implicates distinctive regulatory roles for Id proteins during meiosis, spermatogenesis, and Sertoli cell function. Cell Growth Differ. 1998, 9: 1015-1024.PubMed
6.
Lin CQ, Singh J, Murata K, Itahana Y, Parrinello S, Liang SH, Gillett CE, Campisi J, Desprez PY: A role for Id-1 in the aggressive phenotype and steroid hormone response of human breast cancer cells. Cancer Res. 2000, 60: 1332-1340.PubMed
7.
Wilson JW, Deed RW, Inoue T, Balzi M, Becciolini A, Faraoni P, Potten CS, Norton JD: Expression of Id helix–loop–helix proteins in colorectal adenocarcinoma correlates with p53 expression and mitotic index. Cancer Res. 2001, 61: 8803-8810.PubMed
8.
Parrinello S, Lin CQ, Murata K, Itahana Y, Singh J, Krtolica A, Campisi J, Desprez PY: Id-1, ITF-2, and Id-2 comprise a network of helix–loop–helix proteins that regulate mammary epithelial cell proliferation, differentiation, and apoptosis. J Biol Chem. 2001, 276: 39213-39219. 10.1074/jbc.M104473200.CrossRefPubMed
9.
Lyden D, Young AZ, Zagzag D, Yan W, Gerald W, O'Reilly R, Bader BL, Hynes RO, Zhuang Y, Manova K, Benezra R: Id1 and Id3 are required for neurogenesis, angiogenesis and vascularization of tumour xenografts. Nature. 1999, 401: 670-677. 10.1038/44334.CrossRefPubMed
10.
Shyamala G: Progesterone signaling and mammary gland morphogenesis. J Mammary Gland Biol Neoplasia. 1999, 4: 89-104. 10.1023/A:1018760721173.CrossRefPubMed
11.
Desprez P, Roskelley C, Campisi J, Bissell MJ: Isolation of functional cell lines from a mouse mammary epithelial cell strain: the importance of basement membrane and cell-cell interaction. Mol Cell Differ. 1993, 1: 99-110.
12.
Williams JM, Daniel CW: Mammary ductal elongation: differentiation of myoepithelium and basal lamina during branching morphogenesis. Dev Biol. 1983, 97: 274-290.CrossRefPubMed
13.
Mori S, Nishikawa SI, Yokota Y: Lactation defect in mice lacking the helix–loop–helix inhibitor Id2. EMBO J. 2000, 19: 5772-5781. 10.1093/emboj/19.21.5772.CrossRefPubMedPubMedCentral
14.
Page MJ, Amess B, Townsend RR, Parekh R, Herath A, Brusten L, Zvelebil MJ, Stein RC, Waterfield MD, Davies SC, O'Hare MJ: Proteomic definition of normal human luminal and myoepithelial breast cells purified from reduction mammoplasties. Proc Natl Acad USA. 1999, 96: 12589-12594. 10.1073/pnas.96.22.12589.CrossRef
15.
Lakhani SR, O'Hare MJ: The mammary myoepithelial cell – Cinderella or ugly sister?. Breast Cancer Res. 2001, 3: 1-4. 10.1186/bcr260.CrossRefPubMed
Metadata
Title
Id-1 is not expressed in the luminal epithelial cells of mammary glands
Authors
Norihisa Uehara
Yu-Chien Chou
Jose J Galvez
Paola de-Candia
Robert D Cardiff
Robert Benezra
Gopalan Shyamala
Publication date
01-04-2003
Publisher
BioMed Central
Published in
Breast Cancer Research / Issue 2/2003
Electronic ISSN: 1465-542X
DOI
https://doi.org/10.1186/bcr560

Other articles of this Issue 2/2003

Breast Cancer Research 2/2003 Go to the issue

Viewpoint

New insights into the biological function of BRCA2 from its structural interactions

Research article

Waist–hip ratio and breast cancer risk in urbanized Nigerian women

Commentary

Trastuzumab, an appropriate first-line single-agent therapy for HER2-overexpressing metastatic breast cancer

Research article

Akt kinases in breast cancer and the results of adjuvant therapy

Review

Is there a role for Notch signalling in human breast cancer?

Article selection

Articles selected from Faculty of 1000 in May 2003
Webinar | 19-02-2024 | 17:30 (CET)

Keynote webinar | Spotlight on antibody–drug conjugates in cancer

Watch now

Antibody–drug conjugates (ADCs) are novel agents that have shown promise across multiple tumor types. Explore the current landscape of ADCs in breast and lung cancer with our experts, and gain insights into the mechanism of action, key clinical trials data, existing challenges, and future directions.

Dr. Véronique Diéras
Prof. Fabrice Barlesi
Developed by: Springer Medicine
Image Credits