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Journal of Assisted Reproduction and Genetics

06-05-2024 | ICSI | Assisted Reproduction Technologies

Obstetrical and neonatal outcomes after vitrified-warmed blastocyst transfer in day 1 rescue intracytoplasmic sperm injection cycles: a retrospective cohort study

Authors: Ming Li, Nan Zhang, Jin Huang, Qin Li, JunSheng Li, Rong Li, Ping Liu, Jie Qiao

Published in: Journal of Assisted Reproduction and Genetics

Abstract

Background

Fertilization failure often occurs in conventional IVF cycles, and day 1 rescue ICSI is frequently recommended. In this study, the effect of rescue ICSI on obstetrical and neonatal outcomes after a single blastocyst transfer in vitrified-warmed cycles is evaluated.

Methods

This cohort study was a retrospective analysis of 703 vitrified-warmed single blastocyst transfers and 219 singletons in the r-ICSI group compared with 11,611 vitrified-warmed single blastocyst transfers in the IVF/ICSI and 4472 singletons in the IVF/ICSI group, respectively, and patients just undergoing their first IVF treatments were included in this study. Pregnancy rate (PR), live birth rate (LBR), and singleton birthweight were the primary outcome measures. Multiple linear regression analysis and logistic regression analysis were performed to evaluate the possible relationship between obstetrical and neonatal outcomes and fertilization method (including IVF, ICSI, and r-ICSI) after adjusting for other potential confounding factors.

Results

PR and the LBR were lower in the r-ICSI group compared with the IVF/ ICSI group. Singletons from the r-ICSI group had a higher Z-score and the proportion of large for gestational age (LGA) newborns was greater compared with singletons from the IVF/ICSI group.

Conclusion

The results of the study indicated that a 31% LBR after r-ICSI is acceptable for vitrified-warmed blastocyst transfer, but the safety of transfer is a concern because of the lower PR and LBR compared with IVF/ICSI. The safety of r-ICSI newborns is also a concern because of the significantly higher birthweight and the proportion of LGA in r-ICSI group newborns compared with the IVF/ICSI group.

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Kuczyński W, Dhont M, Grygoruk C, et al. Rescue ICSI of unfertilized oocytes after IVF. Hum Reprod. 2002;17(9):2423–7. https://doi.org/10.1093/humrep/17.9.2423.CrossRefPubMed

Lombardi E, Tiveron M, Inza R, et al. Live birth and normal 1-year follow-up of a baby born after transfer of cryopreserved embryos from rescue intracytoplasmic sperm injection of 1-day-old oocytes. Fertil Steril. 2003;80(3):646–8. https://doi.org/10.1016/s0015-0282(03)00996-8.CrossRefPubMed

Yuzpe AA, Liu Z, Fluker MR. Rescue intracytoplasmic sperm injection (ICSI)-salvaging in vitro fertilization (IVF) cycles after total or near-total fertilization failure. Fertil Steril. 2000;73(6):1115–9. https://doi.org/10.1016/s0015-0282(00)00522-7.CrossRefPubMed

DeUgarte CM, Li M, Jordan B, et al. Rescue intracytoplasmic sperm injection and preimplantation genetic diagnosis in combination can result in pregnancy. Fertil Steril. 2006;86(1):200–2. https://doi.org/10.1016/j.fertnstert.2006.03.021.CrossRefPubMed

Ming L, P Liu, J Qiao, et al. Synchronization between embryo development and endometrium is a contributing factor for rescue ICSI outcome. Reprod Biomed Online. 2012; 24(5): 527–31. https://doi.org/10.1016/j.rbmo.2012.02.001.

Sermondade N, Hugues JN, Cedrin-Durnerin I, et al. Should all embryos from day 1 rescue intracytoplasmic sperm injection be transferred during frozen-thawed cycles? Fertil Steril. 2010;94(3):1157–8. https://doi.org/10.1016/j.fertnstert.2009.12.001.CrossRefPubMed

Li M, Li Q, Wang Y, et al. Blastocyst cryopreservation and cryopreservation-warming transfer is an effective embryo transfer strategy for day 1 rescue intracytoplasmic sperm injection cycles. Sci Rep. 2021;11(1):8260. https://doi.org/10.1038/s41598-021-87693-y.CrossRefPubMedPubMedCentral

Land JA. How should we report on perinatal outcome? Hum Reprod. 2006;21(10):2638–9. https://doi.org/10.1093/humrep/del246.CrossRefPubMed

Liu N, Ma Y, Li R, et al. Comparison of follicular fluid amphiregulin and EGF concentrations in patients undergoing IVF with different stimulation protocols. Endocrine. 2012;42(3):708–16. https://doi.org/10.1007/s12020-012-9706-z.CrossRefPubMed

10.

Palermo G, Joris H, Devroey P, et al. Pregnancies after intracytoplasmic injection of single spermatozoon into an oocyte. Lancet. 1992;340(8810):17–8.CrossRefPubMed

11.

Zheng X, Chen Y, Yan J, et al. Effect of repeated cryopreservation on human embryo developmental potential. Reprod Biomed Online. 2017;35(6):627–32. https://doi.org/10.1016/j.rbmo.2017.08.016.CrossRefPubMed

12.

Chen Y, Zheng X, Yan J, et al. Neonatal outcomes after the transfer of vitrified blastocysts: closed versus open vitrification system. Reprod Biol Endocrinol. 2013;11:107. https://doi.org/10.1186/1477-7827-11-107.CrossRefPubMedPubMedCentral

13.

Roelens C, Santos-Ribeiro S, Becu L, et al. Frozen-warmed blastocyst transfer after 6 or 7 days of progesterone administration: impact on live birth rate in hormone replacement therapy cycles. Fertil Steril. 2020;114(1):125–32. https://doi.org/10.1016/j.fertnstert.2020.03.017.CrossRefPubMed

14.

Villar J, Cheikh Ismail L, Victora CG, et al. International standards for newborn weight, length, and head circumference by gestational age and sex: the Newborn Cross-Sectional Study of the INTERGROWTH-21st Project. Lancet. 2014;384(9946):857–68. https://doi.org/10.1016/S0140-6736(14)60932-6.CrossRefPubMed

15.

Miao YL, Kikuchi K, Sun QY, et al. Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Hum Reprod Update. 2009;15(5):573–85. https://doi.org/10.1093/humupd/dmp014.CrossRefPubMed

16.

Pehlivan T, Rubio C, Ruiz A, et al. Embryonic chromosomal abnormalities obtained after rescue intracytoplasmic sperm injection of 1-day-old unfertilized oocytes. J Assist Reprod Genet. 2004;21(2):55–7. https://doi.org/10.1023/b:jarg.0000025939.26834.93.CrossRefPubMedPubMedCentral

17.

Shimoi G, Tomita M, Kataoka M, et al. Destabilization of spindle assembly checkpoint causes aneuploidy during meiosis II in murine post-ovulatory aged oocytes. J Reprod Dev. 2019;65(1):57–66. https://doi.org/10.1262/jrd.2018-056.CrossRefPubMed

18.

Shimoi G, Wakabayashi R, Ishikawa R, et al. Effects of post-ovulatory aging on centromeric cohesin protection in murine MII oocytes. Reprod Med Biol. 2022; 21(1). https://doi.org/10.1002/rmb2.12433.

19.

Feitosa WB, Morris PL. Post-ovulatory aging is associated with altered patterns for small ubiquitin-like modifier (SUMO) proteins and SUMO-specific proteases. FASEB J. 2023;37(3):e22816. https://doi.org/10.1096/fj.202200622R.CrossRefPubMed

20.

Martin JH, Nixon B, Cafe SL, et al. Oxidative stress and reproductive function: oxidative stress and in vitro ageing of the post-ovulatory oocyte: an update on recent advances in the field. Reproduction. 2022;164(6):F109-f124. https://doi.org/10.1530/rep-22-0206.CrossRefPubMed

21.

Schon EA, Kim SH, Ferreira JC, et al. Chromosomal non-disjunction in human oocytes: is there a mitochondrial connection? Hum Reprod. 2000;15(Suppl 2):160–72. https://doi.org/10.1093/humrep/15.suppl_2.160.CrossRefPubMed

22.

Zhang X, Wu XQ, Lu S, et al. Deficit of mitochondria-derived ATP during oxidative stress impairs mouse MII oocyte spindles. Cell Res. 2006;16(10):841–50. https://doi.org/10.1038/sj.cr.7310095.CrossRefPubMed

23.

Lee AR, Thanh Ha L, Kishigami S, et al. Abnormal lysine acetylation with postovulatory oocyte aging. Reprod Med Biol. 2014;13(2):81–6. https://doi.org/10.1007/s12522-013-0172-y.CrossRefPubMed

24.

Liang X, Ma J, Schatten H, et al. Epigenetic changes associated with oocyte aging. Sci China Life Sci. 2012;55(8):670–6. https://doi.org/10.1007/s11427-012-4354-3.CrossRefPubMed

25.

Liang XW, Ge ZJ, Wei L, et al. The effects of postovulatory aging of mouse oocytes on methylation and expression of imprinted genes at mid-term gestation. Mol Hum Reprod. 2011;17(9):562–7. https://doi.org/10.1093/molehr/gar018.CrossRefPubMed

26.

Petri T, Dankert D, Demond H, et al. In vitro postovulatory oocyte aging affects H3K9 trimethylation in two-cell embryos after IVF. Ann Anat. 2020;227:151424. https://doi.org/10.1016/j.aanat.2019.151424.CrossRefPubMed

27.

Gao Y, Yi L, Zhan J, et al. A clinical study of preimplantation DNA methylation screening in assisted reproductive technology. Cell Res. 2023;33(6):483–5. https://doi.org/10.1038/s41422-023-00809-z.CrossRefPubMed

28.

Smith ZD, Meissner A. DNA methylation: roles in mammalian development. Nat Rev Genet. 2013;14(3):204–20. https://doi.org/10.1038/nrg3354.CrossRefPubMed

29.

Whitelaw N, Bhattacharya S, Hoad G, et al. Epigenetic status in the offspring of spontaneous and assisted conception. Hum Reprod. 2014;29(7):1452–8. https://doi.org/10.1093/humrep/deu094.CrossRefPubMed

30.

Banik A, Kandilya D, Ramya S, et al. Maternal factors that induce epigenetic changes contribute to neurological disorders in offspring. Genes (Basel). 2017; 8(6). https://doi.org/10.3390/genes8060150.

31.

Engel SM, Joubert BR, Wu MC, et al. Neonatal genome-wide methylation patterns in relation to birth weight in the Norwegian Mother and Child Cohort. Am J Epidemiol. 2014;179(7):834–42. https://doi.org/10.1093/aje/kwt433.CrossRefPubMedPubMedCentral

32.

Agha G, Hajj H, Rifas-Shiman SL, et al. Birth weight-for-gestational age is associated with DNA methylation at birth and in childhood. Clin Epigenetics. 2016;8:118. https://doi.org/10.1186/s13148-016-0285-3.CrossRefPubMedPubMedCentral

33.

St-Pierre J, Hivert MF, Perron P, et al. IGF2 DNA methylation is a modulator of newborn’s fetal growth and development. Epigenetics. 2012;7(10):1125–32. https://doi.org/10.4161/epi.21855.CrossRefPubMedPubMedCentral

34.

Carlsen EO, Lee Y, Magnus P, et al. An examination of mediation by DNA methylation on birthweight differences induced by assisted reproductive technologies. Clin Epigenetics. 2022;14(1):151. https://doi.org/10.1186/s13148-022-01381-w.CrossRefPubMedPubMedCentral

35.

Kupers LK, Monnereau C, Sharp GC, et al. Meta-analysis of epigenome-wide association studies in neonates reveals widespread differential DNA methylation associated with birthweight. Nat Commun. 2019;10(1):1893. https://doi.org/10.1038/s41467-019-09671-3.CrossRefPubMedPubMedCentral

36.

Haworth KE, Farrell WE, Emes RD, et al. Methylation of the FGFR2 gene is associated with high birth weight centile in humans. Epigenomics. 2014;6(5):477–91. https://doi.org/10.2217/epi.14.40.CrossRefPubMed

37.

Pelletier A, Carrier A, Zhao Y, et al. Epigenetic and transcriptomic programming of HSC quiescence signaling in large for gestational age neonates. Int J Mol Sci. 2022; 23(13). https://doi.org/10.3390/ijms23137323.

38.

Shen Z, Tang Y, Song Y, et al. Differences of DNA methylation patterns in the placenta of large for gestational age infant. Medicine (Baltimore). 2020;99(39):e22389. https://doi.org/10.1097/MD.0000000000022389.CrossRefPubMedPubMedCentral

39.

Tarin JJ, Perez-Albala S, Aguilar A, et al. Long-term effects of postovulatory aging of mouse oocytes on offspring: a two-generational study. Biol Reprod. 1999;61(5):1347–55. https://doi.org/10.1095/biolreprod61.5.1347.CrossRefPubMed

40.

Tarín JJ, Pérez-Albalá S, Pérez-Hoyos S, et al. Postovulatory aging of oocytes decreases reproductive fitness and longevity of offspring. Biol Reprod. 2002;66(2):495–9. https://doi.org/10.1095/biolreprod66.2.495.CrossRefPubMed

41.

Maheshwari A, Pandey S, Amalraj Raja E, et al. Is frozen embryo transfer better for mothers and babies? Can cumulative meta- analysis provide a definitive answer? Hum Reprod Update. 2018;24:35–58.CrossRefPubMed

42.

Cnattingius S, Villamor E, Lagerros YT, et al. High birth weight and obesity–a vicious circle across generations. Int J Obes (Lond). 2012;36(10):1320–4. https://doi.org/10.1038/ijo.2011.248.CrossRefPubMed

43.

Moore GS, Kneitel AW, Walker CK, et al. Autism risk in small-and large-for-gestational-age infants. Am J Obstet Gynecol. 2012;206(4):314 e1-9. https://doi.org/10.1016/j.ajog.2012.01.044.CrossRefPubMed

44.

Mu M, Wang SF, Sheng J, et al. Birth weight and subsequent blood pressure: a meta-analysis. Arch Cardiovasc Dis. 2012;105(2):99–113. https://doi.org/10.1016/j.acvd.2011.10.006.CrossRefPubMed

45.

Pereira N, Elias RT, Christos PJ, et al. Supraphysiologic estradiol is an independent predictor of low birth weight in full-term singletons born after fresh embryo transfer. Hum Reprod. 2017;32(7):1410–7. https://doi.org/10.1093/humrep/dex095.CrossRefPubMedPubMedCentral

46.

Järvelä IY, Pelkonen S, Uimari O, et al. Controlled ovarian hyperstimulation leads to high progesterone and estradiol levels during early pregnancy. Hum Reprod. 2014;29(11):2393–401. https://doi.org/10.1093/humrep/deu223.CrossRefPubMed

Title: Obstetrical and neonatal outcomes after vitrified-warmed blastocyst transfer in day 1 rescue intracytoplasmic sperm injection cycles: a retrospective cohort study
Authors: Ming Li
Nan Zhang
Jin Huang
Qin Li
JunSheng Li
Rong Li
Ping Liu
Jie Qiao
Publication date: 06-05-2024
Publisher: Springer US
Keyword: ICSI
Published in: Journal of Assisted Reproduction and Genetics
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-024-03126-5

At a glance: The STEP trials

Springer Medicine

Obstetrical and neonatal outcomes after vitrified-warmed blastocyst transfer in day 1 rescue intracytoplasmic sperm injection cycles: a retrospective cohort study

Abstract

Background

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Background

Methods

Results

Conclusion

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