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Open Access 01-02-2014 | Original Article

Hypoxia refines plasticity of mitochondrial respiration to repeated muscle work

Authors: Dominique Desplanches, Myriam Amami, Sylvie Dupré-Aucouturier, Paola Valdivieso, Silvia Schmutz, Matthias Mueller, Hans Hoppeler, Roland Kreis, Martin Flück

Published in: European Journal of Applied Physiology | Issue 2/2014

Abstract

Purpose

We explored whether altered expression of factors tuning mitochondrial metabolism contributes to muscular adaptations with endurance training in the condition of lowered ambient oxygen concentration (hypoxia) and whether these adaptations relate to oxygen transfer as reflected by subsarcolemmal mitochondria and oxygen metabolism in muscle.

Methods

Male volunteers completed 30 bicycle exercise sessions in normoxia or normobaric hypoxia (4,000 m above sea level) at 65 % of the respective peak aerobic power output. Myoglobin content, basal oxygen consumption, and re-oxygenation rates upon reperfusion after 8 min of arterial occlusion were measured in vastus muscles by magnetic resonance spectroscopy. Biopsies from vastus lateralis muscle, collected pre and post a single exercise bout, and training, were assessed for levels of transcripts and proteins being associated with mitochondrial metabolism.

Results

Hypoxia specifically lowered the training-induced expression of markers of respiratory complex II and IV (i.e. SDHA and isoform 1 of COX-4; COX4I1) and preserved fibre cross-sectional area. Concomitantly, trends (p < 0.10) were found for a hypoxia-specific reduction in the basal oxygen consumption rate, and improvements in oxygen repletion, and aerobic performance in hypoxia. Repeated exercise in hypoxia promoted the biogenesis of subsarcolemmal mitochondria and this was co-related to expression of isoform 2 of COX-4 with higher oxygen affinity after single exercise, de-oxygenation time and myoglobin content (r ≥ 0.75). Conversely, expression in COX4I1 with training correlated negatively with changes of subsarcolemmal mitochondria (r < −0.82).

Conclusion

Hypoxia-modulated adjustments of aerobic performance with repeated muscle work are reflected by expressional adaptations within the respiratory chain and modified muscle oxygen metabolism.

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Title: Hypoxia refines plasticity of mitochondrial respiration to repeated muscle work
Authors: Dominique Desplanches
Myriam Amami
Sylvie Dupré-Aucouturier
Paola Valdivieso
Silvia Schmutz
Matthias Mueller
Hans Hoppeler
Roland Kreis
Martin Flück
Publication date: 01-02-2014
Publisher: Springer Berlin Heidelberg
Published in: European Journal of Applied Physiology / Issue 2/2014
Print ISSN: 1439-6319
Electronic ISSN: 1439-6327
DOI: https://doi.org/10.1007/s00421-013-2783-8

At a glance: The STEP trials

Springer Medicine

Hypoxia refines plasticity of mitochondrial respiration to repeated muscle work

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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