Hypothetical mechanism of light action on nitric oxide physiological effects

Nitric oxide is a universal cellular mediator. It is involved in many physiological processes, including those induced by light. The disability for complete analysis of the nitric oxide metabolites in tissues prevents the exact understanding of the role of NO in a particular process. The sensitivity and selectivity of an enzymatic sensor developed in our lab is based on the detection of all NO groups that carry a positive charge or acquire it in the chemical processes. Using this sensor, we have shown that dinitrosyl iron complexes (DNIC), being principal nitric oxide donors in most of living tissues, undergo transformations under light irradiation in the wavelength range of 400–700 nm. These changes are not associated with nitric oxide release to the environment. But a nitrosyl iron complex without thiol ligands (Fe(NO)n) is produced. Moreover, in the moment of the complex reorganization, the chemical bond between the NO group and other components apparently weakens and, in the presence of a substance possessing chemical affinity to the NO group, the latter acquires the ability of transition from the complex to this substance. Therefore, the efficiency of NO donors first of all depends on the existence of the NO target and its status including that under the action of light. The activation of a donor compound by light can facilitate the transfer of NO to the target. Transfer of NO from the donor to the target occurs without releasing NO, or with a minimum time of its stay in the unbound state.