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European Archives of Oto-Rhino-Laryngology

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Open Access 01-02-2020 | Hypopharyngeal Cancer | Head and Neck

Blocking long noncoding RNA MALAT1 restrained the development of laryngeal and hypopharyngeal carcinoma

Authors: Enhong Xu, Xiaoben Liang, Zhenhua Ji, Shuwei Zhao, Li Li, Juntian Lang

Published in: European Archives of Oto-Rhino-Laryngology | Issue 2/2020

Abstract

Purpose

The long non-coding RNA MALAT1 is a predictive marker in several solid tumors with highly conserved sequences. However, the role of non-coding RNA in development of laryngeal or hypopharyngeal cancer remains unclear.

Methods

Tumor tissues and adjacent non-cancer tissues of 24 patients were collected. We detected the expression of MALAT1 in laryngeal cancer tissues and hypopharyngeal cancer tissues. Moreover, we developed a MALAT1 silencing model in human laryngeal tumor cells by transfecting MALAT1 small interfering RNA into human laryngeal carcinoma cell line Hep-2 and pharyngeal carcinoma cell line FaDu with Lipofectamine 2000 system. Cell cycle analysis, Cell Counting Kit-8 assay, Transwell assay, quantitative reverse transcription PCR, and wound-healing assays were performed to evaluate the impact of MALAT1 depletion on laryngeal or hypopharyngeal cancer cell’s growth, proliferation, apoptosis, invasion and migration.

Results

MALAT1 was significantly up-regulated in laryngeal and hypopharyngeal carcinoma cells. MALAT1 down-regulation induced the increased apoptosis of both cell lines and suppressed cells’ proliferation. Cells were arrested in G1/G2 phase and cells of S phase were significantly decreased. Down-regulation of MALAT1 expression can also inhibit the migration and invasion of laryngeal squamous cell carcinoma cell (Hep-2) and hypopharyngeal cancer cell (FaDu).

Conclusion

In summary, our deactivation model of MALAT1 disentangled the active function of it as a regulator of gene expression governing the hallmarks of laryngeal and hypopharyngeal cancer. Blocking this long non-coding RNA may restrain the development of laryngeal cancer.

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Title: Blocking long noncoding RNA MALAT1 restrained the development of laryngeal and hypopharyngeal carcinoma
Authors: Enhong Xu
Xiaoben Liang
Zhenhua Ji
Shuwei Zhao
Li Li
Juntian Lang
Publication date: 01-02-2020
Publisher: Springer Berlin Heidelberg
Keywords: Hypopharyngeal Cancer
Laryngeal Cancer
Hypopharyngeal Carcinoma
Laryngeal Cancer
Published in: European Archives of Oto-Rhino-Laryngology / Issue 2/2020
Print ISSN: 0937-4477
Electronic ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-019-05732-x

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Blocking long noncoding RNA MALAT1 restrained the development of laryngeal and hypopharyngeal carcinoma

Abstract

Purpose

Methods

Results

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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