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Intensive Care Medicine
Published in: Intensive Care Medicine 1/2008

01-01-2008 | Original

Hyperglycemia and lipopolysaccharide decrease depression effect of interleukin 8 production by hypothermia: an experimental study with endothelial cells

Authors: Akihiro Noda, Kosaku Kinoshita, Atsushi Sakurai, Taro Matsumoto, Hideo Mugishima, Katsuhisa Tanjoh

Published in: Intensive Care Medicine | Issue 1/2008

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Abstract

Objective

This study assessed whether hyperglycemia and lipopolysaccharide (LPS) decrease the depression effect of interleukin (IL) 8 production by hypothermia in endothelial cells.

Design and setting

Laboratory study in a university laboratory.

Subjects

Human umbilical vein endothelial cells (HUVECs).

Interventions

HUVECs were cultivated in various concentrations of glucose (5.5 or 16.5 mM = 100 or 300 mg/dl) with or without LPS stimulation for 5, 12, or 24 h at either 30° or 37 °C.

Results

After culturing, IL-8 mRNA expressions and IL-8 levels were measured. At 37 °C, hyperglycemia significantly increased basal IL-8 mRNA at 12 h and basal IL-8 at 24 h. At 37 °C hyperglycemia significantly increased LPS-stimulated IL-8 mRNA at 12 h and LPS-stimulated IL-8 at 12 and 24 h. At 30 °C basal IL-8mRNA, basal IL-8, and LPS-stimulated IL-8 were significantly decreased by hypothermia, but these hypothermic effects were not observed in LPS-stimulated IL-8 mRNA. Furthermore even at 30 °C hyperglycemia significantly increased LPS-stimulated IL-8 mRNA at all time points and LPS stimulated IL-8 at 24 h.

Conclusions

Hypothermia (30 °C) decreases the production of IL-8 in HUVECs but does not decrease the expression of IL-8 mRNA. When hypothermia is followed by hyperglycemia and LPS stimulation, such a combination may expose the patients to a high risk of secondary tissue damage during therapeutic hypothermia.
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Metadata
Title
Hyperglycemia and lipopolysaccharide decrease depression effect of interleukin 8 production by hypothermia: an experimental study with endothelial cells
Authors
Akihiro Noda
Kosaku Kinoshita
Atsushi Sakurai
Taro Matsumoto
Hideo Mugishima
Katsuhisa Tanjoh
Publication date
01-01-2008
Publisher
Springer-Verlag
Published in
Intensive Care Medicine / Issue 1/2008
Print ISSN: 0342-4642
Electronic ISSN: 1432-1238
DOI
https://doi.org/10.1007/s00134-007-0861-2

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