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Neurocritical Care
Published in: Neurocritical Care 1/2019

01-02-2019 | Original Article

Hyperbaric Oxygen Protects Against Cerebral Damage in Permanent Middle Cerebral Artery Occlusion Rats and Inhibits Autophagy Activity

Authors: KongMiao Lu, HaiRong Wang, XiaoLi Ge, QingHua Liu, Miao Chen, Yong Shen, Xuan Liu, ShuMing Pan

Published in: Neurocritical Care | Issue 1/2019

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Abstract

Background

To investigate the effects of hyperbaric oxygen (HBO) on brain damage and autophagy levels in a rat model of middle cerebral artery occlusion.

Methods

Neurologic injury and infarcted areas were evaluated according to the modified neurological severity score and 2,3,5-triphenyltetrazolium chloride staining. Western blots were used to determine beclin1, caspase-3 and fodrin1 protein expression. Beclin1 protein expression (an autophagy marker), positive terminal dUTP nick-end labeling (TUNEL) staining (an apoptosis marker) and positive propidium iodide (PI) staining (a necrosis marker) were detected by immunofluorescence.

Results

Our results indicated that HBO could decrease the infarct volume and speed up the recovery of the neurological deficit scores in ischemic rats. Beclin1 was down-regulated after HBO treatment. HBO treatment inhibited fodrin1 protein expression and decreased the number of PI-positive cells. HBO also down-regulated caspase-3 and decreased the number of TUNEL-positive cells.

Conclusion

Cerebral ischemia caused early neuronal death due to necrosis, followed by delayed neuronal death due to apoptosis. Consequently, autophagy might be involved in all processes of ischemia. HBO could protect the brain against ischemic injury, and the possible mechanisms might be correlated with decreased autophagy activity and decreased apoptosis and necrosis levels.
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Literature
1.
2.
Cheng Y, Deshmukh M, D’Costa A, Demaro JA, Gidday JM, Shah A, et al. Caspase inhibitor affords neuroprotection with delayed administration in a rat model of neonatal hypoxic-ischemic brain injury. J Clin Investig. 1998;101(9):1992–9.CrossRefPubMedPubMedCentral
3.
Gill R, Soriano M, Blomgren K, Hagberg H, Wybrecht R, Miss MT, et al. Role of caspase-3 activation in cerebral ischemia-induced neurodegeneration in adult and neonatal brain. J Cereb Blood Flow Metab. 2002;22(4):420–30.CrossRefPubMed
4.
Joly LM, Mucignat V, Mariani J, Plotkine M, Charriaut-Marlangue C. Caspase inhibition after neonatal ischemia in the rat brain. J Cereb Blood Flow Metab. 2004;24(1):124–31.CrossRefPubMed
5.
Renolleau S, Fau S, Goyenvalle C, Joly LM, Chauvier D, Jacotot E, et al. Specific caspase inhibitor Q-VD-OPh prevents neonatal stroke in P7 rat: a role for gender. J Neurochem. 2007;100(4):1062–71.CrossRefPubMed
6.
7.
Maiuri MC, Zalckvar E, Kimchi A, Kroemer G. Self-eating and self-killing: crosstalk between autophagy and apoptosis. Nat Rev Mol Cell Biol. 2007;8(9):741–52.CrossRefPubMed
8.
9.
10.
Kihara A, Kabeya Y, Ohsumi Y, Yoshimori T. Beclin-phosphatidylinositol 3-kinase complex functions at the trans-Golgi network. EMBO Rep. 2001;2(4):330–5.CrossRefPubMedPubMedCentral
11.
Matchett GA, Martin RD, Zhang JH. Hyperbaric oxygen therapy and cerebral ischemia: neuroprotective mechanisms. Neurol Res. 2009;31(2):114–21.CrossRefPubMed
12.
Nemoto EM, Betterman K. Basic physiology of hyperbaric oxygen in brain. Neurol Res. 2007;29(2):116–26.CrossRefPubMed
13.
Yin X, Meng F, Wang Y, Wei W, Li A, Chai Y, et al. Effect of hyperbaric oxygen on neurological recovery of neonatal rats following hypoxic-ischemic brain damage and its underlying mechanism. Int J Clin Exp Pathol. 2013;6(1):66–75.PubMed
14.
Lin KC, Niu KC, Tsai KJ, Kuo JR, Wang LC, Chio CC, et al. Attenuating inflammation but stimulating both angiogenesis and neurogenesis using hyperbaric oxygen in rats with traumatic brain injury. J Trauma Acute Care Surg. 2012;72(3):650–9.CrossRefPubMed
15.
Wang XL, Yang YJ, Xie M, Yu XH, Liu CT, Wang X. Proliferation of neural stem cells correlates with Wnt-3 protein in hypoxic-ischemic neonate rats after hyperbaric oxygen therapy. NeuroReport. 2007;18(16):1753–6.CrossRefPubMed
16.
Yan W, Zhang H, Bai X, Lu Y, Dong H, Xiong L. Autophagy activation is involved in neuroprotection induced by hyperbaric oxygen preconditioning against focal cerebral ischemia in rats. Brain Res. 2011;1402:109–21.CrossRefPubMed
17.
Lu Y, Kang J, Bai Y, Zhang Y, Li H, Yang X, et al. Hyperbaric oxygen enlarges the area of brain damage in MCAO rats by blocking autophagy via ERK1/2 activation. Eur J Pharmacol. 2014;728:93–9.CrossRefPubMed
18.
Rupadevi M, Parasuraman S, Raveendran R. Protocol for middle cerebral artery occlusion by an intraluminal suture method. J Pharmacol Pharmacother. 2011;2(1):36–9.CrossRefPubMedPubMedCentral
19.
Chen J, Sanberg PR, Li Y, Wang L, Lu M, Willing AE, et al. Intravenous administration of human umbilical cord blood reduces behavioral deficits after stroke in rats. Stroke. 2001;32(11):2682–8.CrossRefPubMed
20.
Li Y, Chopp M, Chen J, Wang L, Gautam SC, Xu YX, et al. Intrastriatal transplantation of bone marrow nonhematopoietic cells improves functional recovery after stroke in adult mice. J Cereb Blood Flow Metab. 2000;20(9):1311–9.CrossRefPubMed
21.
Ashwal S, Tone B, Tian HR, Cole DJ, Pearce WJ. Core and penumbral nitric oxide synthase activity during cerebral ischemia and reperfusion. Stroke. 1998;29(5):1037–46.CrossRefPubMed
22.
23.
Saido TC, Yokota M, Nagao S, Yamaura I, Tani E, Tsuchiya T, et al. Spatial resolution of fodrin proteolysis in postischemic brain. J Biol Chem. 1993;268(33):25239–43.PubMed
24.
Northington FJ, Zelaya ME, O’Riordan DP, Blomgren K, Flock DL, Hagberg H, et al. Failure to complete apoptosis following neonatal hypoxia–ischemia manifests as ‘‘continuum” phenotype of cell death and occurs with multiple manifestations of mitochondrial dysfunction in rodent forebrain. Neuroscience. 2007;149(4):822–33.CrossRefPubMed
25.
Puyal J, Vaslin A, Mottier V, Clarke PG. Postischemic treatment of neonatal cerebral ischemia should target autophagy. Ann Neurol. 2009;66(3):378–89.CrossRefPubMed
26.
Hossmann KA. Viability thresholds and the penumbra of focal ischemia. Ann Neurol. 1994;36(4):557–65.CrossRefPubMed
27.
Northington FJ, Ferriero DM, Graham EM, Traystman RJ, Martin LJ. Early neurodegeneration after hypoxia–ischemia in neonatal rat is necrosis while delayed neuronal death is apoptosis. Neurobiol Dis. 2001;8(2):207–19.CrossRefPubMed
28.
Benjelloun N, Joly LM, Palmier B, Plotkine M, Charriaut-Marlangue C. Apoptotic mitochondrial pathway in neurones and astrocytes after neonatal hypoxia–ischaemia in the rat brain. Neuropathol Appl Neurobiol. 2003;29(4):350–60.CrossRefPubMed
29.
30.
Rockswold SB, Rockswold GL, Defillo A. Hyperbaric oxygen in traumatic brain injury. Neurol Res. 2007;29(2):162–72.CrossRefPubMed
31.
Bao DS, Wu YK, Fu SJ, Wang GY, Yang SJ, Liang GB, et al. Hyperbaric oxygenation protects against ischemia-reperfusion injury in transplanted rat kidneys by triggering autophagy and inhibiting inflammatory response. Ann Transplant. 2017;10(22):75–82.CrossRef
32.
Sun Y, Liu D, Su P, Lin F, Tang Q. Changes in autophagy in rats after spinal cord injury and the effect of hyperbaric oxygen on autophagy. Neurosci Lett. 2016;618:139–45.CrossRefPubMed
33.
Chen C, Chen W, Li Y, Dong Y, Teng X, Nong Z, et al. Hyperbaric oxygen protects against myocardial reperfusion injury via the inhibition of inflammation and the modulation of autophagy. Oncotarget. 2017;8(67):111522–34.PubMedPubMedCentral
34.
Adhami F, Liao G, Morozov YM, Schloemer A, Schmithorst VJ, Lorenz JN, et al. Cerebral ischemia hypoxia induces intravascular coagulation and autophagy. Am J Pathol. 2006;169(2):566–83.CrossRefPubMedPubMedCentral
Metadata
Title
Hyperbaric Oxygen Protects Against Cerebral Damage in Permanent Middle Cerebral Artery Occlusion Rats and Inhibits Autophagy Activity
Authors
KongMiao Lu
HaiRong Wang
XiaoLi Ge
QingHua Liu
Miao Chen
Yong Shen
Xuan Liu
ShuMing Pan
Publication date
01-02-2019
Publisher
Springer US
Published in
Neurocritical Care / Issue 1/2019
Print ISSN: 1541-6933
Electronic ISSN: 1556-0961
DOI
https://doi.org/10.1007/s12028-018-0577-x

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