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European Journal of Medical Research
Published in: European Journal of Medical Research 4/2009

01-12-2009 | Research

Hydrogen peroxide release and acid-base status in exhaled breath condensate at rest and after maximal exercise in young, healthy subjects

Authors: E Marek, P Platen, J Volke, K Mückenhoff, W Marek

Published in: European Journal of Medical Research | Special Issue 4/2009

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Abstract

Objective

Exhaled breath condensate (EBC) contains among a large number of mediators hydrogen peroxide (H2O2) as a marker of airway inflammation and oxidative stress. Similarly EBC pH also changes in respiratory diseases. It was the aim of our investigation to prove if hydrogen peroxide release and changes in pH of EBC changes with exercise.

Methods

EBC was collected from 100 litres exhaled air along with samples of arterialized blood of 16 healthy subjects (9 males, 7 females, age 23 ± 1 years). EBC hydrogen peroxide was analyzed with EcoCheck amperometer (FILT, Berlin). The rate of H2O2 release was calculated from the concentration and collection time. pH and PCO2 in blood and in EBC were measured with the Radiometer blood gas analyzer, EBC was equilibrated with a gas mixture (5% CO2 in O2). The bicarbonate concentration was calculated according to the law of mass action for CO2 and HCO3 - (pK = 6.1).

Results

H2O2 concentration in EBC was 190 ± 109 nmol/l, and H2O2 release at rest was 31.0 ± 18.3 pmol/min. At maximal exercise, the H2O = concentration in EBC increased to 250 ± 120 nmol/l, and H2O2 release significantly increased at maximal exercise to 84.4 ± 39.9 pmol/min (P < 0.01). At rest pH of the CO2 equilibrated EBC was at 6.08 ± 0.23 and the [HCO3 -] was 1.03 ± 0.40 mmol/l. At maximum exercise, pH 6.18 ± 0.17 and [HCO3 -] 1.23 ± 0.30 mmol/l remained almost unaltered.

Conclusions

The rate of H2O2 release in EBC increased during exhausting exercise (external load: 300 Watt) by a factor of 2, whereas the pH and the bicarbonate concentration of the EBC, equilibrated with 5% CO2 at 37°C were not significantly altered. It has to be proven by further experiments whether there is a linear relationship between the rates of H2O2 release in EBC in graded submaximal exercise.
Literature
1.
Quanjer P, Tammeling GJ, Cotes JE, Pederson OF, Peslin R, Yernault JC: Lung volumes and forced ventilatory flows. Report of the working party standardization of lung functions tests. European Community for Steel and Coal. Eur Respir J 1993.,6(Suppl 16):
2.
Kohlrausch F: Praktische Physik. Stuttgart, Teubner Verlagsgesellschaft 23rd edition. 1986.
3.
Marek E, Mückenhoff K, Streckert HJ, Becher G, Marek W: Bestimmung von L-Laktat und H 2 O 2 im Atemkondensat in Ruhe und unter leichter bis mittelgradiger Belastung. Pneumologie 2008, 62: 1–7.
4.
Marek E, Mückenhoff K, Streckert HJ, Becher G, Marek W: Bestimmungen von L-Laktat im Atemkondensat in Ruhe so wie bei fahrradergometrischen Belastungsuntersuchungen. Atemw.-Lungenkrkh 2008, 34: 252–260.CrossRef
5.
Kullmann T, Barta I, Lazar Z, Szili B, Barat E, Valyon M, Kollai M, Horvarth I: Exhaled breath condensate pH standardised for CO 2 partial pressure. Eur Respir J 2007, 29: 496–501. 10.1183/09031936.00084006PubMedCrossRef
6.
Horvarth I, Hunt J, Barnes PJ: Exhaled breath condensate: methodological recommendations and unresolved questions. Eur Respir J 2005, 26: 523–548. 10.1183/09031936.05.00029705CrossRef
7.
Gerritsen WB, Zanen P, Bauwens AA, van den Bosch JM, Haas FJ: Validation of a new method to measure hydrogen peroxide in exhaled breath condensate. Respir Med 2005, 99: 1132–1137. 10.1016/j.rmed.2005.02.020PubMedCrossRef
8.
Loukides S, Bouros D, Papatheodorou G, Lachanis S, Panagou P, Siafakas NM: Exhaled H 2 O 2 in steady-state bronchiectasis: relationship with cellular composition in induced sputum, spirometry, and extent and severity of disease. Chest 2002,121(1):81–87. 10.1378/chest.121.1.81PubMedCrossRef
9.
Nowak D, Kalucka S, Bialasiewicz P, Krol M: Exhalation of H 2 O 2 and thiobarbituric acid reactive substances (TBARs) in healthy subjects. Free Radic Biol Med 2001, 30: 178–186. 10.1016/S0891-5849(00)00457-3PubMedCrossRef
10.
Svensson S, Ölin AC, Lärstad M, Ljungkvist G, Torén K: Determination of hydrogen peroxide in exhaled breath condensate by flow injection analysis with fluorescence detection. J Chromatogr B Analyt Technol Biomed Life Sci 2004,809(2):199–203.PubMedCrossRef
11.
Knobloch H, Becher G, Decker M, Reinhold P: Evaluation of H 2 O 2 and pH in exhaled breath condensate samples: methodical and physiological aspects. Biomarkers 2008,13(3):319–341. 10.1080/13547500701831440PubMedCrossRef
12.
Conner GE, Salathe M, Forteza R: Lactoperoxidase and hydrogen peroxide metabolism in the airways. Am J Respir Crit Care Med 2002,166(12):S57-S61. 10.1164/rccm.2206018PubMedCrossRef
13.
Nowak D, Antczak A, Krol M, Pietras T, Shariati B, Bialasiewicz P, Jeczkowski K, Kula P: Increased content of hydrogen peroxide in the expired breath of cigarette smokers. Eur Respir J 1996,9(4):652–657. 10.1183/09031936.96.09040652PubMedCrossRef
14.
Shephard RJ: Physiology and Biochemistry of exercise. New York: Praeger Publishers; 1982.
15.
van Beurden WJ, Dekhuijzen PN, Harff GA, Smeenk FW: Variability of exhaled hydrogren peroxide in stable COPD patients and matched healthy controls. Respiration 2002,69(3):211–216. 10.1159/000063622PubMedCrossRef
16.
Dohlmann AW, Black HR, Royall JA: Expired breath hydrogen peroxide is a marker of acute airway inflammation. Am Rev Respir Dis 1993, 148: 955–960. 10.1164/ajrccm/148.4_Pt_1.955CrossRef
17.
Antczak A, Kurmanowska Z, Kasielski M, Nowak D: Increased hydrogen peroxide and thiobarbituric acid-reactive products in expired breath condensate of asthmatic patients. Eur Respir J 1997, 10: 1235–1241. 10.1183/09031936.97.10061235PubMedCrossRef
18.
Dekhuijzen PN, Aben KK, Dekker I, Aarts LP, Wielders PL, van Herwaarden CL, Bast A: Increased exhalation of hydrogen peroxide in patients with stable and unstable chronic obstructive pulmonary disease. Am J Respir Crit Care Med 1996,154(3):813–816.PubMedCrossRef
19.
Nowak D, Kasielski M, Pietras T, Bialasiewicz P, Antczak A: Cigarette smoking does not increase hydrogen peroxide levels in expired breath condensate of patients with stable COPD. Monaldi Arch Chest Dis 1998,53(3):268–273.PubMed
20.
Kasielski M, Nowak D: Long term administration of Nacetylcysteine decreases hydrogen peroxide exhalation in subjects with chronic obstructive pulmonary disease. Respir Med 2001,95(6):448–456. 10.1053/rmed.2001.1066PubMedCrossRef
21.
De Benedetto F, Aceto A, Dragani B, Spacone A, Formisano S, Cocco R, Sanguinetti CM: Validation of a new technique to assess exhaled hydrogen peroxide: results from normals and COPD patients. Monaldi Arch Chest Dis 2000,55(3):185–188.PubMed
22.
Loukides S, Horvarth I, Wodehouse T, Cole PJ, Barnes PJ: Elevated levels of exipired breath hydrogen peroxide in bronchiectasis. Am J Respir Crit Care Med 1998,158(3):991–1004.PubMedCrossRef
23.
Worlitz D, Herberth G, Ulrich M, Döring G: Catalase, myeoperoxide and hydrogen peroxide in cystic fibrosis. Eur Respir J 1998,11(2):377–383. 10.1183/09031936.98.11020377CrossRef
24.
Baldwin SR, Simon RH, Grum CM, Ketai LH, Boxer LA, Devall LJ: Oxidant activity in expired breath of patients with adult respiratory stress syndrome. Lancet 1986, 18471: 11–14.CrossRef
25.
Heard SO, Longtine K, Toth I, Puyana JC, Potenza B, Smyrnios N: The influence of liposome-encapsulated Prostaglandin E1 on hydrogen peroxide on hydrogen peroxide concentrations in the exhaled breath of patients with the acute respiratory stress syndrome. Anesth Analg 1999,89(2):353–357.PubMed
26.
Horvarth I, Donnelly LE, Kiss A, Kharitonow SA, Lim S, Fan Chung K, Barnes PJ: Combined use of exhaled hydrogen peroxide and nitric oxide in monitoring asthma. Am J Respir Crit Care Med 1998,158(4):1042–1046.CrossRef
27.
Lachance PA, Nakat Z, Jeong WS: Antioxidants: an integrative approach. Nutrition 2001, 17: 835–838. 10.1016/S0899-9007(01)00636-0PubMedCrossRef
28.
Sen CK: Antioxidant and redox regulation of cellular signalling. Med Sci Sports Exerc 2001,33(3):368–370. 10.1097/00005768-200103000-00005PubMedCrossRef
29.
Schmidt MC, Askev EW, Roberts DE, Prior RL, Ensign WY Jr, Hesslink RE Jr: Oxidative stress in humans training in a cold, moderate altitude environment and their response to a phytochemical atioxidant supplement. Wilderness Environ Med 2002, 13: 94–105. 10.1580/1080-6032(2002)013[0094:OSIHTI]2.0.CO;2PubMedCrossRef
30.
Heinicke I, Boehler A, Rechsteiner T, Bogdanova A, Jelkmann W, Hofer M, Rawlings P, Araneda OF, Behn C, Gassmann M: Moderate altitude but not additional endurance training increases markers of oxidative stress in exhaled breath condensate. Eur J Appl Physiol Epub 2009.
31.
Ricciardolo FLM, Rado V, Fabbri LM, Sterk PJ, Di Maria GU, Geppeti P: Bronchoconstriction induced by citric acid inhalation in guinea pigs: role of tachykinins, bradykinin, and nitric oxide. Am J Respir Crit Care Med 1999, 159: 557–562.PubMedCrossRef
32.
Luk CK, Dulfano MJ: Effect of pH, viscosity and ionic strength changes in ciliary beating frequency of human bronchial explants. Clin Sci Lond 1983, 64: 449–451.PubMed
33.
Holma B, Hegg PO: pH and protein-dependent buffer capacity and viscosity of respiratory mucus: their interrelationship and influence on health. Sci Total Environ 1989, 84: 71–82.PubMedCrossRef
34.
Holma B, Lindgren M, Andersen JM: pH effects on ciliomotility and morphology of respiratory mucosa. Arch Environ Health 1977, 32: 216–226.PubMedCrossRef
35.
Kostikas K, Papatheodorou G, Ganas K, Psathakis K, Panagou P, Loukides S: pH in expired breath condensate of patients with inflammatory airway diseases. Am J Respir Crit Care Med 2002, 165: 1364–1370. 10.1164/rccm.200111-068OCPubMedCrossRef
36.
Vaughan J, Ngamtrakulparit L, Pajewski TN: Exhaled breath condensate pH is a robust and reproducibly assay of airway acidity. Eur Respir J 2003, 22: 889–894. 10.1183/09031936.03.00038803PubMedCrossRef
37.
Riedecker M, Danuser B: Exhaled breath condensate pH is increased after moderate exercise. J Aerosol Med 2007,20(1):13–18. 10.1089/jam.2006.0567CrossRef
38.
Effros RM, Biller J, Foss B, Hoagland K, Dunning MS, Castillo D, Bosbous M, Sun F, Shaker R: A simple method for estimating respiratory solute dilution in exhaled breath condensates. Am J Respir Crit Care Med 2003, 168: 1500–1505. 10.1164/rccm.200307-920OCPubMedCrossRef
Metadata
Title
Hydrogen peroxide release and acid-base status in exhaled breath condensate at rest and after maximal exercise in young, healthy subjects
Authors
E Marek
P Platen
J Volke
K Mückenhoff
W Marek
Publication date
01-12-2009
Publisher
BioMed Central
Published in
European Journal of Medical Research / Issue Special Issue 4/2009
Electronic ISSN: 2047-783X
DOI
https://doi.org/10.1186/2047-783X-14-S4-134

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