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Molecular Imaging and Biology
Published in: Molecular Imaging and Biology 4/2020

01-08-2020 | Hyaluronic Acid | Research Article

Nanoparticle Formulation of Indocyanine Green Improves Image-Guided Surgery in a Murine Model of Breast Cancer

Authors: Nicholas E. Wojtynek, Madeline T. Olson, Timothy A. Bielecki, Wei An, Aaqib M. Bhat, Hamid Band, Scott R. Lauer, Edibaldo Silva-Lopez, Aaron M. Mohs

Published in: Molecular Imaging and Biology | Issue 4/2020

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Abstract

Purpose

Negative surgical margins (NSMs) have favorable prognostic implications in breast tumor resection surgery. Fluorescence image-guided surgery (FIGS) has the ability to delineate surgical margins in real time, potentially improving the completeness of tumor resection. We have recently developed indocyanine green (ICG)-loaded self-assembled hyaluronic acid (HA) nanoparticles (NanoICG) for solid tumor imaging, which were shown to enhance intraoperative contrast.

Procedures

This study sought to assess the efficacy of NanoICG on completeness of breast tumor resection and post-surgical survival. BALB/c mice bearing iRFP+/luciferase+ 4T1 syngeneic breast tumors were administered NanoICG or ICG, underwent FIGS, and were compared to bright light surgery (BLS) and sham controls.

Results

NanoICG increased the number of complete resections and improved tumor-free survival. This was a product of improved intraoperative contrast enhancement and the identification of a greater number of small, occult lesions than ICG and BLS. Additionally, NanoICG identified chest wall invasion and predicted recurrence in a model of late-stage breast cancer.

Conclusions

NanoICG is an efficacious intraoperative contrast agent and could potentially improve surgical outcomes in breast cancer.
Appendix
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Metadata
Title
Nanoparticle Formulation of Indocyanine Green Improves Image-Guided Surgery in a Murine Model of Breast Cancer
Authors
Nicholas E. Wojtynek
Madeline T. Olson
Timothy A. Bielecki
Wei An
Aaqib M. Bhat
Hamid Band
Scott R. Lauer
Edibaldo Silva-Lopez
Aaron M. Mohs
Publication date
01-08-2020
Publisher
Springer International Publishing
Published in
Molecular Imaging and Biology / Issue 4/2020
Print ISSN: 1536-1632
Electronic ISSN: 1860-2002
DOI
https://doi.org/10.1007/s11307-019-01462-y

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