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Journal of Neuroinflammation
Published in: Journal of Neuroinflammation 1/2004

Open Access 01-12-2004 | Research

Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs

Authors: Masato Hosokawa, Andis Klegeris, Patrick L McGeer

Published in: Journal of Neuroinflammation | Issue 1/2004

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Abstract

Background

Oligodendrocytes, neurons, astrocytes, microglia, and endothelial cells are capable of synthesizing complement inhibitor proteins. Oligodendrocytes are vulnerable to complement attack, which is particularly observed in multiple sclerosis. This vulnerability may be related to a deficiency in their ability to express complement regulatory proteins.

Methods

This study compared the expression level of complement inhibitor mRNAs by human oligodendrocytes, astrocytes and microglia using semi-quantitative RT-PCR.

Results

Semi-quantitative RT-PCR analysis showed that C1 inhibitor (C1-inh) mRNA expression was dramatically lower in oligodendroglial cells compared with astrocytes and microglia. The mRNA expression level of membrane cofactor protein (MCP) by oligodendrocytes was also significantly lower than for other cell types.

Conclusion

The lower mRNA expression of C1-inh and MCP by oligodendrocytes could contribute to their vulnerability in several neurodegenerative and inflammatory diseases of the central nervous system.
Appendix
Available only for authorised users
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Metadata
Title
Human oligodendroglial cells express low levels of C1 inhibitor and membrane cofactor protein mRNAs
Authors
Masato Hosokawa
Andis Klegeris
Patrick L McGeer
Publication date
01-12-2004
Publisher
BioMed Central
Published in
Journal of Neuroinflammation / Issue 1/2004
Electronic ISSN: 1742-2094
DOI
https://doi.org/10.1186/1742-2094-1-17

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