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Inflammation Research
Published in: Inflammation Research 1/2018

01-01-2018 | Original Research Paper

Human neutrophils are targets to paracoccin, a lectin expressed by Paracoccidioides brasiliensis

Authors: R. Ricci-Azevedo, R. A. Gonçales, M. C. Roque-Barreira, D. Girard

Published in: Inflammation Research | Issue 1/2018

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Abstract

Objective and design

Paracoccin (PCN), a lectin expressed by Paracoccidioides brasiliensis (Pb), is known to exert activities on the fungal biology, as well as different immune cells of myeloid origin. The aim of this study was to investigate the direct interaction of the recombinant form of the lectin (rPCN) with neutrophils, a neglected area.

Materials or subjects

Freshly isolated human neutrophils from healthy donors were used.

Treatment

Neutrophils were incubated with rPCN in vitro.

Methods

After the treatment, the production of reactive oxygen species (ROS), DNA release, IL-8, TNF, IFN-γ, IL-10, IL-12p40, TGF-β and IL-1β production, fungicidal ability, apoptosis and de novo protein synthesis was determined.

Results

rPCN was found to induce ROS production as well as DNA release. Using the ROS inhibitor, diphenyleneiodium, both ROS production and DNA release were significantly inhibited. In addition, rPCN was found to induce IL-8 and IL1-β production, inhibit apoptosis and induce de novo protein synthesis. Addition of cycloheximide, a protein synthesis inhibitor, drastically reversed the antiapoptotic effect of rPCN. Finally, the ability to kill Pb yeasts by human neutrophils was significantly increased after rPCN stimulation.

Conclusions

rPCN can alter the biology of human neutrophils increasing their fungicidal ability. Moreover, the ability of rPCN to increase DNA release and to induce suppression of neutrophil apoptosis occurs by a ROS- and de novo protein synthesis-dependent mechanism, respectively.
Literature
1.
Akgul C, Moulding DA, Edwards SW. Molecular control of neutrophil apoptosis. FEBS Lett. 2001;487:318–22.CrossRefPubMed
2.
3.
Bordon J, Aliberti S, Fernandez-Botran R, Uriarte SM, Rane MJ, Duvvuri P, et al. Understanding the roles of cytokines and neutrophil activity and neutrophil apoptosis in the protective versus deleterious inflammatory response in pneumonia. Int J Infect Dis. 2012;17(2):e76–83.CrossRefPubMed
4.
Konig MF, Andrade F. A critical reappraisal of neutrophil extracellular traps and NETosis mimics based on differential requirements for protein citrullination. Front Immunol. 2016;7:461.PubMedPubMedCentral
5.
Bonder CS, Ajuebor MN, Zbytnuik LD, Kubes P, Swain MG. Essential role for neutrophil recruitment to the liver in concanavalin A-induced hepatitis. J Immunol. 2004;172:45–53.CrossRefPubMed
6.
Timoshenko AV, Kayser K, Gabius HJ. Lectin-triggered superoxide/h(2)o (2) and granule enzyme release from cells. Methods Mol Med. 1998;9:441–51.PubMed
7.
Lavastre V, Pelletier M, Saller R, Hostanska K, Girard D. Mechanisms involved in spontaneous and Viscum album agglutinin-I-induced human neutrophil apoptosis: viscum album agglutinin-I accelerates the loss of antiapoptotic Mcl-1 expression and the degradation of cytoskeletal paxillin and vimentin proteins via caspases. J Immunol. 2002;168:1419–27.CrossRefPubMed
8.
Savoie A, Lavastre V, Pelletier M, Hajto T, Hostanska K, Girard D. Activation of human neutrophils by the plant lectin Viscum album agglutinin-I: modulation of de novo protein synthesis and evidence that caspases are involved in induction of apoptosis. J Leukoc Biol. 2000;68:845–53.PubMed
9.
Sato S, Ouellet N, Pelletier I, Simard M, Rancourt A, Bergeron MG. Role of galectin-3 as an adhesion molecule for neutrophil extravasation during streptococcal pneumonia. J Immunol. 2002;168:1813–22.CrossRefPubMed
10.
Gong Y, Zhang Y, Feng S, Liu X, Lu S, Long M. Dynamic contributions of P- and E-selectins to beta2-integrin induced neutrophil transmigration. Faseb J. 2016;31(1):212–23.CrossRefPubMed
11.
12.
Futosi K, Fodor S, Mocsai A. Reprint of Neutrophil cell surface receptors and their intracellular signal transduction pathways. Int Immunopharmacol. 2013;17:1185–97.CrossRefPubMed
13.
Futosi K, Fodor S, Mocsai A. Neutrophil cell surface receptors and their intracellular signal transduction pathways. Int Immunopharmacol. 2013;17:638–50.CrossRefPubMedPubMedCentral
14.
Mariano VS, Zorzetto-Fernandes AL, da Silva TA, Ruas LP, Nohara LL, Almeida IC, et al. Recognition of TLR2N-glycans: critical role in ArtinM immunomodulatory activity. PLoS One. 2014;9:e98512.CrossRefPubMedPubMedCentral
15.
Silva TA, Souza MA, Cecilio NT, Roque-Barreira MC. Activation of spleen cells by ArtinM may account for its immunomodulatory properties. Cell Tissue Res. 2014;357:719–30.CrossRefPubMedPubMedCentral
16.
Toledo KA, Scwartz C, Oliveira AF, Conrado MC, Bernardes ES, Fernandes LC, et al. Neutrophil activation induced by ArtinM: release of inflammatory mediators and enhancement of effector functions. Immunol Lett. 2009;123:14–20.CrossRefPubMed
17.
Ricci-Azevedo R, Oliveira AF, Conrado MC, Carvalho FC, Roque-Barreira MC. Neutrophils contribute to the protection conferred by ArtinM against intracellular pathogens: a study on leishmania major. PLoS Negl Trop Dis. 2016;10:e0004609.CrossRefPubMedPubMedCentral
18.
Freitas MS, Oliveira AF, da Silva TA, Fernandes FF, Goncales RA, Almeida F, et al. Paracoccin induces M1 polarization of macrophages via interaction with TLR4. Front Microbiol. 2016;7:1003.PubMedPubMedCentral
19.
Alegre-Maller AC, Mendonca FC, da Silva TA, Oliveira AF, Freitas MS, Hanna ES, et al. Therapeutic administration of recombinant Paracoccin confers protection against Paracoccidioides brasiliensis infection: involvement of TLRs. PLoS Negl Trop Dis. 2014;8:e3317.CrossRefPubMedPubMedCentral
20.
Alegre AC, Oliveira AF, Dos Reis Almeida FB, Roque-Barreira MC, Hanna ES. Recombinant paracoccin reproduces the biological properties of the native protein and induces protective Th1 immunity against Paracoccidioides brasiliensis infection. PLoS Negl Trop Dis. 2014;8:e2788.CrossRefPubMedPubMedCentral
21.
dos Reis Almeida FB, de Oliveira LL, de Valle Sousa M, Roque Barreira MC, Hanna ES. Paracoccin from Paracoccidioides brasiliensis; purification through affinity with chitin and identification of N-acetyl-beta-D-glucosaminidase activity. Yeast. 2010;27:67–76.CrossRefPubMed
22.
de Liz R, Horst H, Pizzolatti MG, Frode TS, Girard D. Activation of human neutrophils by the anti-inflammatory mediator Esenbeckia leiocarpa leads to atypical apoptosis. Mediat Inflamm. 2012;198382:8.
23.
Simard JC, Girard D, Tessier PA. Induction of neutrophil degranulation by S100A9 via a MAPK-dependent mechanism. J Leukoc Biol. 2010;87:905–14.CrossRefPubMed
24.
Binet F, Cavalli H, Moisan E, Girard D. Arsenic trioxide (AT) is a novel human neutrophil pro-apoptotic agent: effects of catalase on AT-induced apoptosis, degradation of cytoskeletal proteins and de novo protein synthesis. Br J Haematol. 2006;132:349–58.CrossRefPubMed
25.
Goncalves DM, Girard D. Zinc oxide nanoparticles delay human neutrophil apoptosis by a de novo protein synthesis-dependent and reactive oxygen species-independent mechanism. Toxicol In Vitro. 2014;28:926–31.CrossRefPubMed
26.
Xu Y, Mahmood M, Fejleh A, Li Z, Watanabe F, Trigwell S, et al. Carbon-covered magnetic nanomaterials and their application for the thermolysis of cancer cells. Int J Nanomed. 2010;5:167–76.CrossRef
27.
Calich VL, Purchio A, Paula CR. A new fluorescent viability test for fungi cells. Mycopathologia. 1979;66:175–7.CrossRefPubMed
28.
Vorobjeva NV, Pinegin BV. Effects of the antioxidants Trolox, Tiron and Tempol on neutrophil extracellular trap formation. Immunobiology. 2016;221:208–19.CrossRefPubMed
29.
Li Y, Trush MA. Diphenyleneiodonium, an NAD(P)H oxidase inhibitor, also potently inhibits mitochondrial reactive oxygen species production. Biochem Biophys Res Commun. 1998;253:295–9.CrossRefPubMed
30.
Burger E, Mendes AC, Bani GM, Brigagao MR, Santos GB, Malaquias LC, et al. Low-level laser therapy to the mouse femur enhances the fungicidal response of neutrophils against Paracoccidioides brasiliensis. PLoS Negl Trop Dis. 2015;9:e0003541.CrossRefPubMedPubMedCentral
31.
Della Coletta AM, Bachiega TF, de Quaglia e Silva JC, Soares AM, De Faveri J, Marques SA, et al. Neutrophil extracellular traps identification in tegumentary lesions of patients with paracoccidioidomycosis and different patterns of NETs generation in vitro. PLoS Negl Trop Dis. 2015;9:e0004037.CrossRefPubMedPubMedCentral
32.
Vonk AG, Netea MG, van Krieken JH, Iwakura Y, van der Meer JW, Kullberg BJ. Endogenous interleukin (IL)-1 alpha and IL-1 beta are crucial for host defense against disseminated candidiasis. J Infect Dis. 2006;193:1419–26.CrossRefPubMed
33.
Tavares AH, Magalhaes KG, Almeida RD, Correa R, Burgel PH, Bocca AL. NLRP3 inflammasome activation by Paracoccidioides brasiliensis. PLoS Negl Trop Dis. 2013;7:e2595.CrossRefPubMedPubMedCentral
34.
Silva JS, Twardzik DR, Reed SG. Regulation of Trypanosoma cruzi infections in vitro and in vivo by transforming growth factor beta (TGF-beta). J Exp Med. 1991;174:539–45.CrossRefPubMed
35.
Hirsch CS, Yoneda T, Averill L, Ellner JJ, Toossi Z. Enhancement of intracellular growth of Mycobacterium tuberculosis in human monocytes by transforming growth factor-beta 1. J Infect Dis. 1994;170:1229–37.CrossRefPubMed
36.
Shao X, Rivera J, Niang R, Casadevall A, Goldman DL. A dual role for TGF-beta1 in the control and persistence of fungal pneumonia. J Immunol. 2005;175:6757–63.CrossRefPubMed
37.
Welin A, Amirbeagi F, Christenson K, Bjorkman L, Bjornsdottir H, Forsman H, et al. The human neutrophil subsets defined by the presence or absence of OLFM4 both transmigrate into tissue in vivo and give rise to distinct NETs in vitro. PLoS one. 2013;8:e69575.CrossRefPubMedPubMedCentral
38.
Poirier M, Simard JC, Girard D. Silver nanoparticles of 70 and 20 nm affect differently the biology of human neutrophils. J Immunotoxicol. 2015;13:375–85.CrossRefPubMed
39.
Antoine F, Simard JC, Girard D. Curcumin inhibits agent-induced human neutrophil functions in vitro and lipopolysaccharide-induced neutrophilic infiltration in vivo. Int Immunopharmacol. 2013;17:1101–7.CrossRefPubMed
40.
Pelletier M, Roberge CJ, Gauthier M, Vandal K, Tessier PA, Girard D. Activation of human neutrophils in vitro and dieldrin-induced neutrophilic inflammation in vivo. J Leukoc Biol. 2001;70:367–73.PubMed
Metadata
Title
Human neutrophils are targets to paracoccin, a lectin expressed by Paracoccidioides brasiliensis
Authors
R. Ricci-Azevedo
R. A. Gonçales
M. C. Roque-Barreira
D. Girard
Publication date
01-01-2018
Publisher
Springer International Publishing
Published in
Inflammation Research / Issue 1/2018
Print ISSN: 1023-3830
Electronic ISSN: 1420-908X
DOI
https://doi.org/10.1007/s00011-017-1093-8

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