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BMC Oral Health
Published in: BMC Oral Health 1/2016

Open Access 01-12-2016 | Research article

HSP70 mRNA expression by cells of the epithelial rest of Malassez due to mechanical forces in vitro

Authors: Hideki Kogai, Kei Nakajima, Tungalag Ser-Od, Akram Al-Wahabi, Kenichi Matsuzaka, Taneaki Nakagawa, Takashi Inoue

Published in: BMC Oral Health | Issue 1/2016

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Abstract

Background

The purpose of the present study was to examine the in vitro responses of ERM cells under the combination of centrifugal and compression forces, in terms of their expression of HSP70 mRNA.

Methods

The ERM cells were positive for CK19 indicating that they were derived from the odontogenic epithelium. Cultured ERM cells were applied centrifugal force and compressing force at one to three times as mechanical forces. After addition of forces, cells were observed using scanning electron microscope (SEM) and were measured expression of HSP70 mRNA by RT-PCR.

Results

SEM observations showed the cells were flattened immediately after the application of mechanical force, but nuclear protrusions recovered the same as the control 3 h later. A significantly higher expression of HSP70 mRNA was observed in ERM cells under mechanical force compared with the control, but it gradually decreased with time. No accumulation of HSP70 mRNA expression occurred with intermittent force. However, the expression of HSP70 mRNA with intermittent force repeated 3 times was significantly higher compared with intermittent force applied only once or twice.

Conclusions

These findings suggest that ERM cells express HSP70 mRNA in response to mechanical force, and that intermittent force maintains the level of HSP70 mRNA expression.
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Metadata
Title
HSP70 mRNA expression by cells of the epithelial rest of Malassez due to mechanical forces in vitro
Authors
Hideki Kogai
Kei Nakajima
Tungalag Ser-Od
Akram Al-Wahabi
Kenichi Matsuzaka
Taneaki Nakagawa
Takashi Inoue
Publication date
01-12-2016
Publisher
BioMed Central
Published in
BMC Oral Health / Issue 1/2016
Electronic ISSN: 1472-6831
DOI
https://doi.org/10.1186/s12903-016-0181-4

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