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Open Access 01-06-2016 | Original Article

How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days

Authors: I. de Laak–de Vries, A. G. Siebers, L. Burgers, C. Diepenbroek, M. Link, P. Groenen, J. H. J. M. van Krieken, K. M. Hebeda

Published in: Journal of Hematopathology | Issue 2/2016

Abstract

Since the introduction of fast diagnostic tracks in many areas of oncology, the traditional processing of bone marrow biopsies (BMB), requiring either resin embedding or lengthy fixation and decalcification, is due to an upgrade. Thanks to a growing number of new commercially available tissue processors, microwave-enhanced processing is becoming a standard tool in the pathology laboratory, allowing rapid fixation and decalcification of BMB with preserved morphology and antigens. In this short report, we describe the use of a commercially available EDTA-based decalcification fluid (USEDECALC, Medite, Orlando, USA) in combination with the LOGOS J (Milestone, Bergamo, Italy), a closed microwave-enhanced tissue processor, for overnight fixation, decalcification, and paraffin impregnation of the BMB. This allows next-day reporting without impaired morphology or immunohistochemistry, and even improved DNA quality of the BMB.

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Title: How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days
Authors: I. de Laak–de Vries
A. G. Siebers
L. Burgers
C. Diepenbroek
M. Link
P. Groenen
J. H. J. M. van Krieken
K. M. Hebeda
Publication date: 01-06-2016
Publisher: Springer Berlin Heidelberg
Published in: Journal of Hematopathology / Issue 2/2016
Print ISSN: 1868-9256
Electronic ISSN: 1865-5785
DOI: https://doi.org/10.1007/s12308-016-0270-y

At a glance: The STEP trials

Springer Medicine

How we do: optimizing bone marrow biopsy logistics for sign-out within 2 days

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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