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Published in:

01-09-2010

High Resolution Melting Curve Analysis of DNA Sequence Alterations of Various Sizes

Authors: Péter Becságh, Katalin Varga, Orsolya Szakács, László Kopper, Zsolt Orosz

Published in: Pathology & Oncology Research | Issue 3/2010

Abstract

In the diagnostic workflow we need to think in algorithms, containing more assays. One of the most important task in the management of cancer patient is to detect nucleic acid sequence changes in clinical specimens. Before using the most expensive method to analyze direct our targets, a screening assay is needed to reduce the number of samples. In the detection of gene-sequence alterations classical screening methods are available, as SSCP, DGGE or TGGE, (Finke Exp Clin Endocrinol Diabetes 104:92–97, 1996; Lessa and Applebaum Mol Ecol 2:119–129, 1993) however these are very time consuming processes. At this time in the molecular lab the real-time PCR equipments are very popular and with the function of melting curve analysis it can be a very convenient, simple and cost-efficient screening method.

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Title: High Resolution Melting Curve Analysis of DNA Sequence Alterations of Various Sizes
Authors: Péter Becságh
Katalin Varga
Orsolya Szakács
László Kopper
Zsolt Orosz
Publication date: 01-09-2010
Publisher: Springer Netherlands
Published in: Pathology & Oncology Research / Issue 3/2010
Print ISSN: 1219-4956
Electronic ISSN: 1532-2807
DOI: https://doi.org/10.1007/s12253-010-9274-6

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