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BMC Cancer
Published in: BMC Cancer 1/2006

Open Access 01-12-2006 | Research article

High-resolution analysis of HLA class I alterations in colorectal cancer

Authors: Jan Willem F Dierssen, Noel FCC de Miranda, Arend Mulder, Marjo van Puijenbroek, Willem Verduyn, Frans HJ Claas, Cornelis JH van de Velde, Gert Jan Fleuren, Cees J Cornelisse, Willem E Corver, Hans Morreau

Published in: BMC Cancer | Issue 1/2006

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Abstract

Background

Previous studies indicate that alterations in Human Leukocyte Antigen (HLA) class I expression are frequent in colorectal tumors. This would suggest serious limitations for immunotherapy-based strategies involving T-cell recognition. Distinct patterns of HLA surface expression might conceal different immune escape mechanisms employed by the tumors and are worth further study.

Method

We applied four-color multiparameter flow cytometry (FCM), using a large panel of alloantigen-specific anti-HLA-A and -B monoclonal antibodies, to study membranous expression of individual HLA alleles in freshly isolated colorectal cancer cell suspensions from 21 patients.

Results

Alterations in HLA class I phenotype were observed in 8 (38%) of the 21 tumors and comprised loss of a single A or B alleles in 4 cases, and loss of all four A and B alleles in the other 4 cases. Seven of these 8 tumors were located on the right side of the colon, and those showing loss of both HLA-A and -B membranous expression were all of the MSI-H phenotype.

Conclusion

FCM allows the discrimination of complex phenotypes related to the expression of HLA class I. The different patterns of HLA class I expression might underlie different tumor behavior and influence the success rate of immunotherapy.
Appendix
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Metadata
Title
High-resolution analysis of HLA class I alterations in colorectal cancer
Authors
Jan Willem F Dierssen
Noel FCC de Miranda
Arend Mulder
Marjo van Puijenbroek
Willem Verduyn
Frans HJ Claas
Cornelis JH van de Velde
Gert Jan Fleuren
Cees J Cornelisse
Willem E Corver
Hans Morreau
Publication date
01-12-2006
Publisher
BioMed Central
Published in
BMC Cancer / Issue 1/2006
Electronic ISSN: 1471-2407
DOI
https://doi.org/10.1186/1471-2407-6-233

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