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01-12-2003 | Original Paper

High prevalence of epidermodysplasia verruciformis-associated human papillomavirus DNA in actinic keratoses of the immunocompetent population

Authors: Herbert Pfister, Pawel G. Fuchs, Slawomir Majewski, Stefania Jablonska, Iwona Pniewska, Magdalena Malejczyk

Published in: Archives of Dermatological Research | Issue 7/2003

Abstract

Skin cancers in both immunosuppressed and immunocompetent populations are associated with epidermodysplasia verruciformis human papillomavirus (EV-HPV) DNA. However, little is known about the prevalence of EV-HPVs in actinic keratoses in immunocompetent individuals. Actinic keratoses from 114 patients were classified as low-grade (n=76) or high-grade (n=38) according to the extent of histological atypia. HPV DNA was amplified from 54 frozen and 60 paraffin-embedded biopsy specimens by nested polymerase chain reaction (PCR) with several consensus and type-specific primers. PCR products were sequenced for typing. These results were compared with HPV detection in skin cancers (n=20) and Bowen’s disease (n=18). A broad spectrum of EV-HPV types including oncogenic HPV5 and HPV8 and partially characterized sequences were detected in actinic keratoses and cutaneous cancers. In actinic keratoses a higher prevalence of EV-HPV DNA was found in frozen tissues than in formalin-fixed tissues (85% vs 67%). There was no difference between the low- and high-grade actinic keratoses either in terms of EV-HPV DNA prevalence or the results of serological study using HPV8 virus-like particles. The detection rate of EV-HPVs was lower in skin cancers and Bowen’s disease. This would suggest involvement of EV-HPVs in the early stages of cutaneous oncogenesis.

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Title: High prevalence of epidermodysplasia verruciformis-associated human papillomavirus DNA in actinic keratoses of the immunocompetent population
Authors: Herbert Pfister
Pawel G. Fuchs
Slawomir Majewski
Stefania Jablonska
Iwona Pniewska
Magdalena Malejczyk
Publication date: 01-12-2003
Publisher: Springer-Verlag
Published in: Archives of Dermatological Research / Issue 7/2003
Print ISSN: 0340-3696
Electronic ISSN: 1432-069X
DOI: https://doi.org/10.1007/s00403-003-0435-2

Keynote webinar | Spotlight on medication adherence

Springer Medicine

High prevalence of epidermodysplasia verruciformis-associated human papillomavirus DNA in actinic keratoses of the immunocompetent population

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

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